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Refrigerated Centrifuge with Heating System Sigma 6-16KHS

6 Using the centrifuge

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Version 10/2012, Rev. 1.19 of 21/03/2019

• sb

Translation of the original operating manual, part no. 0702302



6.3.2.5

Standard menu

The "Standard" menu is symbolised by the icon " " on the menu bar and it
is displayed a few seconds after the centrifuge has been switched on. In
this menu, the parameters of a centrifugation run can be displayed and
modified.

Fig. 13: Standard menu; here shown with all of the possible symbols

Speed

In the upper section of the field, the set speed of the centrifuge is displayed.
The actual speed is displayed below this value. The values are stated in
revolutions per minute (min

-1

= rpm) and they depend on the RCF values

(see chapter 2.2.2.1 - "Speed, radius, and relative centrifugal force"). The
maximum speed values depend on the rotor that is used.

Relative centrifugal force (RCF)

The relative centrifugal force is the acceleration that the sample is
subjected to during the centrifugation run. The set value of this parameter is
displayed in the upper section of this field, with the actual value shown
below. The values are stated in g (gravitational acceleration) and they
depend on the speed values (see chapter 2.2.2.1 - "Speed, radius, and
relative centrifugal force"). The maximum RCF values depend on the rotor
that is used.

Temperature

The set temperature is displayed in the upper section of the field, with the
current sample temperature shown below. Temperatures between
-20°C and +60°C can be preselected. The maximum rotor temperature
depends on the rotor that is used.

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Содержание 6-16KHS

Страница 1: ...e original operating manual sb 10 2012 Rev 1 19 of 21 03 2019 Sigma 6 16KHS part no 0702302 Sigma 6 16KHS from serial no 147199 Refrigerated Centrifuge with Heating System Operating Manual Please reta...

Страница 2: ...Refrigerated Centrifuge with Heating System Sigma 6 16KHS 2 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302...

Страница 3: ...original operating manual part no 0702302 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Oster...

Страница 4: ...Refrigerated Centrifuge with Heating System Sigma 6 16KHS 4 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302...

Страница 5: ...2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety instructions...

Страница 6: ...2 Installation of an angle rotor with a hermetic lid 29 6 2 2 3 Installation of accessories 31 6 2 2 4 Adapters 33 6 2 2 5 Vessels 33 6 2 2 6 Blood bag systems 34 6 2 2 7 Centrifugation with mineral o...

Страница 7: ...tic accessories 65 8 1 3 Rotors buckets and carriers 65 8 1 4 Load bearing bolts 66 8 1 5 Glass breakage 67 8 2 Sterilisation and disinfection of the rotor chamber and accessories 67 8 2 1 Autoclaving...

Страница 8: ...Refrigerated Centrifuge with Heating System Sigma 6 16KHS Table of contents 8 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302...

Страница 9: ...1 2 Intended use Centrifuges are power driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any othe...

Страница 10: ...ust not be duplicated distributed or communicated in any other way Non compliance may be prosecuted under criminal law 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC de...

Страница 11: ...n of the original operating manual part no 0702302 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1 User int...

Страница 12: ...t no 0702302 2 1 2 Name plate 1 Manufacturer and registered office 2 Type 3 Serial number 4 Refrigerant data 5 Max density 6 Nominal voltage 7 Input fuse 8 Symbol for special disposal see chapter 9 Di...

Страница 13: ...as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also i...

Страница 14: ...tated formula If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be a...

Страница 15: ...age I On Power Hot surface 0 Off Power Caution Risk of bruising Name plate see chapter 2 1 2 Name plate Protective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not...

Страница 16: ...direct hazard to the life and health of persons Non observance of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life...

Страница 17: ...intended see chapter 1 2 Intended use take protective measures against fire and explosion when working with hazardous substances take measures for the safe opening of centrifuges 3 4 Operating personn...

Страница 18: ...tion Do not reach into the rotor chamber when the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the centrifuge without panels Do not use the centrifuge if the...

Страница 19: ...r radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines precautions and practices are adhered...

Страница 20: ...y warn against the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2...

Страница 21: ...st be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the engraving max cycles...

Страница 22: ...ling conclusions to be drawn concerning the production process and the subsequent quality inspection Some rotors also have an additional serial number providing further detailed information The batch...

Страница 23: ...and sensor signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as error messages in a dialog box see chapter 7 2 Table of error codes 3 7 4 Earth conductor c...

Страница 24: ...9 Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules However danger to life and limb of the operator or of...

Страница 25: ...for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If you would like to store it for more than one year or if you intend to ship it ov...

Страница 26: ...ain the packaging for any possible future transport of the centrifuge 4 5 Transport safety device The transport safety device consists of two plastic screws which are located at the bottom panel CAUTI...

Страница 27: ...en surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the centrif...

Страница 28: ...ened To close press with both hands slightly on the lid until both locks are audibly locked WARNING Do not place your fingers between the lid and the housing when closing the lid Risk of crushing 6 2...

Страница 29: ...an angle rotor with a hermetic lid Open the centrifuge lid by pressing the lid key Unscrew the rotor tie down screw from the motor shaft counter clockwise Screw the hermetic lid onto the rotor and ti...

Страница 30: ...03 2019 sb Translation of the original operating manual part no 0702302 Removing a rotor Loosen the rotor tie down screw anti clockwise and remove the rotor together with the lid Loosen the lid by tu...

Страница 31: ...ll of the compartments must be equipped with buckets Always load rotationally symmetrical compartments of the rotors with the same accessories and fill to avoid imbalance Centrifugation with vessels o...

Страница 32: ...ermissible to load angle rotors on only one axis correct incorrect Fig 9 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations Double rectangular bucket 1...

Страница 33: ...ds in the buckets or multiple carriers cause corrosion Fill the vessels carefully and arrange them according to their weight Imbalances result in the excessive wear of the bearings Always fill the tub...

Страница 34: ...lled blood bag systems must have an equal weight The blood bags have to be put into the opposite buckets mirror inverted If in one bucket the main blood bag is on the left side then the main blood bag...

Страница 35: ...lasma with buffy coat 22 3 750 4 544 9 20 2 min 12 4 Full blood 20 4 000 5 170 14 24 3 min 12 5 Plasma with buffy coat 22 1 094 384 7 21 2 min 11 or 2 min 6 Platelets with buffy coat 22 1 094 384 7 21...

Страница 36: ...knob The display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start button 2 Display 3 Function knob 4 Sto...

Страница 37: ...be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Quick...

Страница 38: ...n 1 rpm and they depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal force The maximum speed values depend on the rotor that is used Relative centrifugal force RCF The r...

Страница 39: ...peed is reached Select the field Runtime and press the function knob The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 cloc...

Страница 40: ...unction knob in order to accept the data Fig 14 Rotor selection list here for a swing out rotor Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being...

Страница 41: ...Standard menu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode...

Страница 42: ...No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message...

Страница 43: ...ides a quick overview of the remaining runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 17 Progress indicator...

Страница 44: ...t be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be displayed If more than 11 programs have been stored the user can s...

Страница 45: ...ifuge to a certain temperature prior to starting a centrifugation The temperature adaptation prevents the samples with a specific temperature in the uncooled centrifuge from heating or cooling down to...

Страница 46: ...ward facing arrow in the speed field Values between 1 2 and 10 0 g cm3 are possible Centrifugation monitoring The centrifugation monitoring function enables the continuous monitoring of the speed and...

Страница 47: ...ed if the current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message wil...

Страница 48: ...the Parameters function is selected the Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be dis...

Страница 49: ...Fig 22 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens when the rotor has stopped CAUTION When the lid is open the cooling is not active The sampl...

Страница 50: ...erature can be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follo...

Страница 51: ...ifuge The letters and characters can be entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the kno...

Страница 52: ...field Curve If the curve number is already used the stored curve will be displayed The input field Int is used to specify the interval number of the process Up to ten intervals can be entered for a cu...

Страница 53: ...e maximum possible speed depending on the rotor and from the slope limitation Example 1 Start speed 0 min 1 rpm final speed 100 min 1 rpm runtime 1 hour not possible since the necessary slope 0 03 min...

Страница 54: ...rogram contains all of the data that are required for a centrifuge run Certain sedimentation results can be repeated under identical conditions Programs can be loaded executed edited and deleted when...

Страница 55: ...r to select a letter and press it to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK...

Страница 56: ...Select the desired program from the list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start butt...

Страница 57: ...gram is loaded while the program rotation function is active this program will be used as the start program for the rotation After the completion of the program the next program on the program list wi...

Страница 58: ...02 6 3 4 Options for data input and output Connection for a serial interface depending on the centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 2...

Страница 59: ...ses Power off on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly Open and close the lid If the error occurs again contac...

Страница 60: ...with a screwdriver Fig 31 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be guided through a funnel shaped tube t...

Страница 61: ...to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance monitori...

Страница 62: ...of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentr...

Страница 63: ...ve substances Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8...

Страница 64: ...essories special safety measures must be considered as these are measures that will ensure operational safety at the same time Immediately rinse off the rotor buckets or accessories under running wate...

Страница 65: ...o withstand the permanent high stress from high gravitational fields Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the...

Страница 66: ...e centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bolts and bucket groove in order to remove the old grease Ap...

Страница 67: ...chamber In order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minut...

Страница 68: ...me min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carri...

Страница 69: ...cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to ageing This is why we recommend having...

Страница 70: ...se we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the...

Страница 71: ...part no 0702302 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuita...

Страница 72: ...it is not permissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declar...

Страница 73: ...consumption kW Max current consumption A Input fuse AT 2 8 2 3 14 0 at 220 240 V 50 Hz 16 0 at 220 240 V 50 Hz temperature fuses Performance data Max speed rpm Max capacity ml Max gravitational field...

Страница 74: ...inal voltage of 100V or 200V a tolerance of 10 5 applies For indoor use only Allowable ambient temperature 5 C to 35 C Max allowable relative humidity of air 80 from 5 C up to 31 C with a linear decre...

Страница 75: ...ccessories come supplied together with a data sheet that includes important information and notes on safety This data sheet must be added to the operating manual 11 1 1 Rotor radii The information in...

Страница 76: ...Heating System Sigma 6 16KHS 11 Appendix 76 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302 11 2 Speed gravitational field diagram Fig 36 Spee...

Страница 77: ...the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power The runtime until the set speed is reached depends solely on the moment of...

Страница 78: ...with Heating System Sigma 6 16KHS 11 Appendix 78 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302 Fig 39 Diagram of linear curves Fig 40 Diagr...

Страница 79: ...Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 14 1 14K 12096...

Страница 80: ...0 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl...

Страница 81: ...iaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylanili...

Страница 82: ...2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1...

Страница 83: ...1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene...

Страница 84: ...Refrigerated Centrifuge with Heating System Sigma 6 16KHS 11 Appendix 84 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302...

Страница 85: ...erated Centrifuge with Heating System Sigma 6 16KHS 11 Appendix Version 10 2012 Rev 1 19 of 21 03 2019 sb 85 93 Translation of the original operating manual part no 0702302 11 6 EC declaration of conf...

Страница 86: ...Refrigerated Centrifuge with Heating System Sigma 6 16KHS 11 Appendix 86 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302...

Страница 87: ...d Centrifuge with Heating System Sigma 6 16KHS 11 Appendix Version 10 2012 Rev 1 19 of 21 03 2019 sb 87 93 Translation of the original operating manual part no 0702302 11 7 Declaration of conformity C...

Страница 88: ...Refrigerated Centrifuge with Heating System Sigma 6 16KHS 11 Appendix 88 93 Version 10 2012 Rev 1 19 of 21 03 2019 sb Translation of the original operating manual part no 0702302...

Страница 89: ...entrifugation of blood bag systems 34 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation with low capacity 32 Centrifugation with min...

Страница 90: ...ssage 59 Executing a program 56 Explanation of the symbols and notes 16 Explosive substances 19 External signal 51 F Filling quantity refrigerant 73 Fire preventions 19 Form for the return of defectiv...

Страница 91: ...cation mode active 37 Motor error 61 Motor shaft 28 29 63 Multiple carrier 33 N Name plate 11 12 No indication on the display 59 No power in the mains supply 59 Noise level 73 Nominal voltage 12 Notes...

Страница 92: ...instructions for centrifugation 20 Safety of rotors and accessories 21 Safety chemical and biological 19 Safety electrical 18 Safety mechanical 18 Safety conscious work 17 Saving a program 55 Scope o...

Страница 93: ...23 Temperature monitoring 47 Temperature monitoring system 23 Temperature unit 50 Temperature value not reached 59 Thermal stress 63 Time fine 50 Time range 73 Toxic substances 19 63 Transport safety...

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