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Sigma 4-5KL, Руководство по эксплуатации

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Refrigerated centrifuge Sigma 4-5KL 

Refrigerated centrifuge Sigma 4-5KL IVD 

6 Using the centrifuge 

 

 

 

Version 06/2020, Rev. 1.2 of 28/04/2022 

• sb 

49 / 84 

Translation of the original operating manual, part no. 0703602 

 

→

 

6.3.3 

Program mode 

A program contains all the data that are required for a centrifuge run. 
Certain sedimentation results can be repeated under identical conditions. 

Programs can be saved, loaded, executed, and edited when the centrifuge 
is at a standstill. 

A maximum of 50 programs can be stored under the numbers 1 - 50. The 
rapid cooling program "Rapid Temp" does not occupy any storage location 
and cannot be deleted. It is used to cool the centrifuge without any vessels. 

"--" means that the values that are currently set are not a stored program. 

The programs can be protected against unauthorised use, modification, or 
deletion with the aid of an input lock (see chapter 6.3.2.14 - "Input lock"). 

 

 

→

 

→

 

6.3.3.1 

Saving a program 

 

NOTE 

This function is only available if the centrifuge is at a standstill. 

 

 
•   Enter the parameters that are to be included in the program. 

 

 

•  Select the menu item "save Prog" and confirm the selection. The 

indication "save" flashes in the activated status. 

•  Select a random storage location from the program selection list. Free 

storage locations are indicated by a flashing display. Any storage 
locations that are already occupied will be overwritten during the saving 
process. 

•  Save the program in the desired location. 
The program is now saved. 

 

NOTE 

The functions "Auto Open", "Buzzer", "ProgLock", and "Cycles" cannot be 
entered as part of a program. 

 

 

Fig. 32: Saving a program 

 

 

Содержание 4-5KL

Страница 1: ...sb 06 2020 Rev 1 2 of 28 04 2022 Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD Refrigerated centrifuge part no 0703602 Sigma 4 5KL Sigma 4 5KL IVD from serial no 180163 Refrigerated centrifuge...

Страница 2: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 2 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602...

Страница 3: ...Rev 1 2 of 28 04 2022 sb 3 84 Translation of the original operating manual part no 0703602 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522...

Страница 4: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 4 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602...

Страница 5: ...3 2 Explanation of the symbols and notes 17 3 3 Responsibility of the operator 18 3 4 Requirements concerning the personnel 19 3 5 Informal safety instructions 20 3 6 Safety instructions 21 3 6 1 Elec...

Страница 6: ...2 2 1 Installation of a standard rotor 34 6 2 2 2 Installation of accessories 35 6 2 2 3 Adapters 36 6 2 2 4 Vessels 36 6 3 Control system Spincontrol L 37 6 3 1 User interface 37 6 3 2 Manual mode 38...

Страница 7: ...sories 57 8 1 3 Rotors buckets and carriers 57 8 1 4 Load bearing bolts 58 8 1 5 Glass breakage 59 8 2 Sterilisation and disinfection of the rotor chamber and accessories 59 8 2 1 Autoclaving 60 8 3 S...

Страница 8: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD Table of contents 8 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602...

Страница 9: ...ked with IVD is intended for human biological samples including donated blood and tissue in conjunction with diagnostic in vitro applications This means that it is a medical product in accordance with...

Страница 10: ...nder criminal law 1 5 Standards and regulations These operating instructions have been created in accordance with the relevant European standards and regulations see chapter 11 6 EC declaration of con...

Страница 11: ...anslation of the original operating manual part no 0703602 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1...

Страница 12: ...ed centrifuge Sigma 4 5KL IVD 2 Layout and mode of operation 12 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602 5 Circuit breaker 6 Mains power...

Страница 13: ...703602 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9 Consult operating manual 10 Symbol for special disp...

Страница 14: ...tically as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the roto...

Страница 15: ...way of the stated formula If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the sp...

Страница 16: ...ate see chapter 6 2 2 2 Installation of accessories CE mark in compliance with the directive 2006 42 EC Do not dispose as part of domestic waste Consult operating manual Medical product in accordance...

Страница 17: ...uses serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols c...

Страница 18: ...ator is obliged to take measures in order to prevent danger to life or health during work ensure that the centrifuges are used properly and entirely as intended see chapter 1 2 Intended use take prote...

Страница 19: ...e present in the danger zone or work area This manual uses the following personnel qualifications for various areas of activity Qualified electrician Due to their special training knowledge experience...

Страница 20: ...nt user or other person c a serious risk to public health 3 5 Informal safety instructions This operating manual is a part of the product The operating manual must be kept at the location of use of th...

Страница 21: ...e housing needs to be removed Inspect the electrical equipment of the unit regularly Defects such as loose or burnt cables must be eliminated immediately Following the completion of any type of repair...

Страница 22: ...ced immediately Open the centrifuge when it is not in use so that moisture can evaporate 3 6 3 Fire prevention DANGER Do not spin explosive or inflammable substances Do not use the centrifuge within h...

Страница 23: ...xplicitly warn against the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chap...

Страница 24: ...ycles the rotor must be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the en...

Страница 25: ...service life WARNING Perform regular checks at least once per month for safety reasons Pay special attention to changes such as corrosion cracks material abrasion etc The rotors and accessories must...

Страница 26: ...receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection Some rotors also have an additional serial number providing further det...

Страница 27: ...signals with regard to plausibility The system continuously performs a self check and identifies malfunctions Error messages are displayed as Error followed by a code number see chapter 7 2 Table of...

Страница 28: ...doctor 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules However danger to life and limb of the operat...

Страница 29: ...ackaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If you would like to store it for more than one year or if you intend to s...

Страница 30: ...sible future transport of the centrifuge 4 5 Transport safety device The transport safety device consists of two hexagon socket screws which are located at the bottom panel CAUTION The transport safet...

Страница 31: ...r heat generators Avoid direct sunlight UV radiation The table must be stable and have a solid even surface Attention During transport from cold to warmer places condensational water will collect insi...

Страница 32: ...t breaker Switch the unit off by actuating the mains power switch If it has tripped let the circuit breaker cool for approximately 2 minutes Switch the unit on The centrifuge is now ready for operatio...

Страница 33: ...5 Set up and connection 6 2 Switching the centrifuge on Press the mains power switch The display then illuminates The centrifuge is ready for operation 6 2 1 Opening and closing the lid The lid can b...

Страница 34: ...he rotor tie down screw clockwise with the supplied rotor wrench with 10 Nm Option torque wrench 10 Nm part no 17060 In doing so hold the rotor at its outer rim Follow the safety instructions and haza...

Страница 35: ...ies and fill to avoid imbalance Centrifugation with different tube sizes Working with different tube sizes is possible In this case however it is very important that axial symmetric inserts are identi...

Страница 36: ...evenly Loading the edges of a bucket only is not permissible 6 2 2 4 Vessels Load the vessels outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosion Fill the vessels car...

Страница 37: ...iodes and one function knob The display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start key 2 Function k...

Страница 38: ...new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered fast will be displayed i...

Страница 39: ...values depend on the rotor that is used The parameters speed and RCF can be changed during the centrifugation Fig 14 Setting the speed value or the RCF value 6 3 2 6 Runtime The set runtime is display...

Страница 40: ...n this case the symbol disappears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed on the symbol...

Страница 41: ...isplayed Fig 18 Indication Short during a short run 6 3 2 7 Temperature The set value is displayed in the upper area of the field and the actual sample temperature is displayed in the lower area Tempe...

Страница 42: ...the rapid cooling process During the rapid cooling process the set temperature can be modified within the range below the actual temperature The Rapid Temp program will be stopped under the following...

Страница 43: ...ts the various combinations will be displayed one after the other Confirm the input The selected rotor or rotor bucket combination will be adopted Fig 21 Preselection of a rotor or a rotor bucket comb...

Страница 44: ...ection of a curve here preselection of an acceleration curve 6 3 2 10 Automatic lid opening function AutoOpen The automatic lid opening function must be activated so that the lid opens automatically a...

Страница 45: ...pears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed over the symbol Fig 24 The sound signal Bu...

Страница 46: ...Cycle display for rotor 11650 and bucket 13421 Reaching the maximum number of cycles When the maximum number of cycles is reached for a rotor or bucket a corresponding warning signal will be displayed...

Страница 47: ...d hold for approximately 2 seconds when pressing it for the third time After the activation of this function the padlock symbol flashes The input lock is activated Proceed in the same manner in order...

Страница 48: ...knob to position the cursor in the time field so that Set will be displayed Press the start button three times and hold it for approximately 2 seconds when pressing it the last time The display shows...

Страница 49: ...that the values that are currently set are not a stored program The programs can be protected against unauthorised use modification or deletion with the aid of an input lock see chapter 6 3 2 14 Input...

Страница 50: ...ding a program Select the menu item run Prog and confirm the selection The indication run flashes in the activated status Select the desired program and confirm the selection by pressing the function...

Страница 51: ...Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several possible causes Power off on If the error occurs again contact service Centrifuge cannot be started...

Страница 52: ...ft side of the control panel e g with a screwdriver Fig 34 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be guide...

Страница 53: ...f Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance...

Страница 54: ...e event of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sig...

Страница 55: ...aggressive substances Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Main...

Страница 56: ...f the accessories special safety measures must be considered as these are measures that will ensure operational safety at the same time Immediately rinse off the rotor buckets or accessories under run...

Страница 57: ...n order to withstand the permanent high stress from high gravitational fields Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or des...

Страница 58: ...on of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bolts and bucket groove in order to remove the old g...

Страница 59: ...and rotor chamber In order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a...

Страница 60: ...n Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate rou...

Страница 61: ...gh quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other...

Страница 62: ...is purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner...

Страница 63: ...g manual part no 0703602 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us i...

Страница 64: ...eans that it is not permissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in...

Страница 65: ...plate I 20 Power consumption kW Input fuse AT 1 91 16 0 at 220 240 V 50 Hz 16 0 at 200 V 50 60 Hz Temperature fuses Performance data Max speed rpm Max capacity ml Max gravitational field x g Max kine...

Страница 66: ...At a nominal voltage of 100V or 200V a tolerance of 10 5 applies For indoor use only Allowable ambient temperature 5 C to 35 C Max allowable relative humidity of air 80 from 5 C up to 31 C with a line...

Страница 67: ...es The complete list of accessories can be downloaded from www sigma zentrifugen de 11 1 1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respec...

Страница 68: ...frigerated centrifuge Sigma 4 5KL IVD 11 Appendix 68 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602 11 2 Speed gravitational field diagram Fig...

Страница 69: ...rates brakeless spin out In general the runtime until the set speed is reached depends on the moment of inertia of the rotor Linear curves The slope of the fixed acceleration curves defines the time t...

Страница 70: ...S 6 16S 6 16HS 6 16KS 6 16KHS 9366 15 000 4 5KL 4 16S 4 16KS 4 16KHS 6 16S 6 16HS 6 16KS 6 16KHS 11805 10 years 8KS 8KBS 11806 10 years 8KS 8KBS 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084...

Страница 71: ...C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride...

Страница 72: ...3 1 2 1 Diaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dime...

Страница 73: ...O 100 1 2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1...

Страница 74: ...0 1 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1...

Страница 75: ...ntrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 11 Appendix Version 06 2020 Rev 1 2 of 28 04 2022 sb 75 84 Translation of the original operating manual part no 0703602 11 6 EC declaration...

Страница 76: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 11 Appendix 76 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602...

Страница 77: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 11 Appendix Version 06 2020 Rev 1 2 of 28 04 2022 sb 77 84 Translation of the original operating manual part no 0703602...

Страница 78: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 11 Appendix 78 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602...

Страница 79: ...uge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 11 Appendix Version 06 2020 Rev 1 2 of 28 04 2022 sb 79 84 Translation of the original operating manual part no 0703602 11 7 Declaration of conf...

Страница 80: ...Refrigerated centrifuge Sigma 4 5KL Refrigerated centrifuge Sigma 4 5KL IVD 11 Appendix 80 84 Version 06 2020 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703602...

Страница 81: ...fuge decelerates during operation 51 Centrifuge is inclined 51 Centrifuge was moved during run 51 Centrifuge cleaning and care 55 Centrifuge service life 25 Changes in colour 60 Changes in structure 6...

Страница 82: ...GWP 65 Gravitational field 65 Grease for load bearing bolts 55 58 H Hazard warnings 9 34 36 Heavy duty grease for load bearing bolts 58 Highly corrosive substances 22 I Imbalance 35 36 Imbalance error...

Страница 83: ...ss error 53 ProgLock program lock 45 Program Rapid Temp 42 Program lock ProgLock 45 Protection class 31 65 Q Qualified electrician 19 Quick stop 38 Quitting the modification mode 38 R Radioactive subs...

Страница 84: ...terilisation of the rotor chamber and accessories 59 Stopping of the centrifuge due to an imbalance 58 Storage and transport 29 Storage conditions 29 Storage locations 65 Stress corrosion see corrosio...

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