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Sigma 1-14, Руководство по эксплуатации

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1-14K

 

 

Maintenance and Service 

 

Version 05/2010, Rev. 1.8 of 01/09/2011- sb 

 

39 

Translation of the original operating manual 

8.2.1.  Autoclaving 

The service life of the accessories essentially depends on the 
frequency of autoclaving and use. 

•

 

Replace the accessories immediately when the parts show 
changes in color or structure or in the occurrence of leaks etc.  

•

 

During autoclaving, the caps of the tubes must not be screwed on 
in order to avoid the deformation of the tubes. 

It cannot be excluded that plastic parts, e.g. lids or carriers, may 
deform during autoclaving. 

 

Accessories 

max. temp. 
(°C) 

min. time  max. time  max.  

cycles 

Glass tubes 

134-138 

3 

40 

- 

Polycarbonate tubes 

115-118 

30 

40 

20 

Polypropylene copolymer tubes 

115-121 

30 

40 

30 

Teflon tubes 

134-138 

3 

5 

100 

Aluminium rotors 

134-138 

3 

20 

- 

Polycarbonate/Polyallomer lids for angle rotors 

115-118 

30 

40 

20 

Polysulfone lids for angle rotors 

134-138 

3 

5 

100 

Aluminium buckets 

134-138 

3 

20 

- 

Polysulfone caps for buckets 

134-138 

3 

5 

100 

Rubber adapter 

115-118 

30 

40 

- 

Round carriers made of polypropylene 

115-118 

30 

40 

- 

ditto, made of polyallomer and polycarbonate 

115-118 

30 

40 

- 

Rectangular carriers made of polypropylene 

115-118 

30 

40 

- 

ditto, made of polyallomer and polycarbonate 

115-118 

30 

40 

- 

Fig. 8.1: Autoclaving table 

Содержание 1-14

Страница 1: ...Operating Manual Refrigerated Centrifuge 1 14K from serial no 135550 Please retain for later use Version 05 2010 Rev 1 8 of 01 09 2011 sb Translation of the original operating manual...

Страница 2: ......

Страница 3: ...by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Phone 49 0 55 22 50 07 0 Fax 49 0 55 22 50 07 12 E mail info sigma zentrifugen de For service please use our servi...

Страница 4: ......

Страница 5: ...13 3 3 Responsibility of the Operator 14 3 4 Operating Personnel 14 3 5 Informal Safety Instructions 14 3 6 Safety Instructions 15 3 6 1 Electrical Safety 15 3 6 2 Mechanical Safety 15 3 6 3 Fire Prev...

Страница 6: ...ation 26 6 3 1 Operating Panel 26 6 3 2 Centrifuge Display 26 6 3 3 Starting a Centrifugation Run 27 6 3 4 Interrupting a Centrifugation Run 27 6 3 5 Interrupting a Deceleration Process 27 6 3 6 Speed...

Страница 7: ...Accessories 38 8 2 1 Autoclaving 39 8 3 Service 40 8 4 Return of defective parts 41 9 Disposal 42 9 1 Disposal of the Centrifuge 42 9 2 Disposal of the Packaging 42 10 Technical Data 43 10 1 Ambient C...

Страница 8: ......

Страница 9: ...r driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyond this area of application...

Страница 10: ...any othe way neither in full nor in parts Non compliance may be prosecuted under criminal law 1 5 Standards and Regulations EC Declaration of Conformity page 53 1 6 Scope of Supply The centrifuge comp...

Страница 11: ...9 Mains power input 10 Mains switch 11 Name Plate see 2 2 Fig 2 2 Rear view of the centrifuge 2 2 Name Plate 1 Manufacturer and registered office 2 Type name 3 Serial number 4 Max speed 5 Kinetic ene...

Страница 12: ...Ein Netzverbindung On Power Marche mise sous tension O Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Liaison la terre Erde Earth ground Terre Netz...

Страница 13: ...e endangering injuries This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life enda...

Страница 14: ...as intended see chapter 1 2 Intended Use page 9 of this Operating Manual take measures for the safe opening of centrifuges 3 4 Operating Personnel Persons operating the unit must be familiar with the...

Страница 15: ...en the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the centrifuge without panels Do not use the centrifuge if the rotors and inserts show signs of corrosion...

Страница 16: ...e used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines precautions and practices are adhered to accordingly Spin infectious materia...

Страница 17: ...itted see 6 2 2 1 Installation of the Rotor Observe the instructions on the installation of accessories see 6 2 2 3 The rotor must be loaded symmetrically at equal weights If liquids with a density 1...

Страница 18: ...ch is in ON position and the lid is properly closed When the lid lock is locked the lid can only be opened when the rotor has stopped In the event of a power failure it is possible to open the lid man...

Страница 19: ...3 9 Remaining Hazards The unit was built state of the art and according to the accepted safety rules Danger to life and limb of the operator or of third parties or impairments of the unit or other mat...

Страница 20: ...p to a year without any problems Store the centrifuge only in dry rooms The storage temperature must be above 20 C If you would like to store it for more than one year or if you intend to ship it over...

Страница 21: ...t Safety Device The centrifuge is not eqiupped with a transport safety device 5 3 Installation Site Operate the centrifuge only in closed and dry rooms All the energy supplied to the centrifuge is con...

Страница 22: ...r Supply 5 4 1 Connection The operating voltage on the name plate must correspond to the local supply voltage This SIGMA laboratory centrifuge is a unit of safety class I and has a three wire power co...

Страница 23: ...The centrifuge is ready for operation 6 2 1 Opening and Closing the Lid The lid can be opened if the centrifuge is at a standstill Press the lid key in order to open the lid The centrifuge cannot be...

Страница 24: ...tor with its central bore straight down onto the motor shaft Hand tighten the rotor fastening nut clockwise corresponds to 1 Nm The rotor fastening nut and conical spring washer must face the microhem...

Страница 25: ...essories and fill to avoid imbalance Centrifugation with low capacity The tubes must be installed symmetrically so that the buckets and their inserts are loaded evenly fig 6 2 Permissible loading of a...

Страница 26: ...perating Panel The centrifuge is started directly via the operating panel When the centrifuge is switched on all segments will be illuminated for a short time It is now ready for operation 6 3 2 Centr...

Страница 27: ...and speed The RCF and speed values therefore depend on each other If one of the two values is entered the other value will be set automatically To preselect a speed value or a RCF value press the set...

Страница 28: ...changed during the run the centrifuge will run for the entire new time and will disregard the previous runtime that has already elapsed 6 3 7 2 Short Run During the short run the centrifuge accelerat...

Страница 29: ...ntly cause increased wear of the mechanical components This is why the centrifuge has a special program that precools the centrifuge under defined conditions Press the precool key to load the precooli...

Страница 30: ...key until Soft off is displayed The softstart and softstop functions are now deactivated The data will be accepted immediately and saved after approx 20 seconds You can also change the settings as des...

Страница 31: ...et key repeatedly until the corresponding unit flashes on the display Select the desired parameters In order to save the data start the centrifuge or press the set key repeatedly until the indication...

Страница 32: ...sages with the lid key Type of error Possible reason Correction No power in the mains supply Check fuse in the mains supply Power cord is not plugged in Plug in power cord correctly Mains power switch...

Страница 33: ...located above the tabletop If necessary the unit must be secured by a second person Insert the wrench for the emergency release into the hole see fig 7 2 item 1 and attach it to the shaft of the lid...

Страница 34: ...ated centrifuges allow to slow down power off on allow to cool down provide better ventilation 46 49 Imbalance error allow to slow down power off eliminate the imbalance 50 59 Lid error press lid key...

Страница 35: ...ct SIGMA Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 84 25 Fax 49 0 55 22 50 07 94 25 E mail service sigma zentrifugen de outside Germany contact our agenc...

Страница 36: ...e agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8 1 1 Centrifuge Disconnect the power cord f...

Страница 37: ...fety if there is a risk of toxic radioactive or pathogenic contamination Dry the accessories with a soft cloth or in a drying chamber at approx 50 C 8 1 2 1 Plastic Accessories The chemical resistance...

Страница 38: ...e part no 16002 for rotor 11026 8 2 Sterilization and Disinfection of the Rotor Chamber and Accessories Use commercially available disinfectants such as for example Sagrotan Buraton or Terralin availa...

Страница 39: ...rs may deform during autoclaving Accessories max temp C min time max time max cycles Glass tubes 134 138 3 40 Polycarbonate tubes 115 118 30 40 20 Polypropylene copolymer tubes 115 121 30 40 30 Teflon...

Страница 40: ...t to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years in the dismantled state Rubb...

Страница 41: ...cialised personnel only Affix the original form in a clearly visible manner to the outside of the packaging If no such declaration is enclosed in the return package we will perform the decontamination...

Страница 42: ...ot permissible to dispose of the unit among household trash You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of...

Страница 43: ...768 Other parameters Time range Temperature range 10 sec 99 min 59 sec short run continuous run 10 C to 40 C Physical data Height mm Height with opened lid mm Width mm Depth mm Weight kg EMC as per E...

Страница 44: ...02 12094 Angle rotor made of polypropylene 24 x 1 5 2 0 ml for reaction vials e g no 15008 15040 incl polysulfone lid no 17882 angle 42 48 radius max 6 6 cm radius min 3 8 and 3 0 cm 15 000 16 602 120...

Страница 45: ...005 Reaction vials 0 5 ml 100 pcs per pack 15008 Reaction vials 1 5 ml 100 pcs per pack 15040 Reaction vials 2 0 ml 100 pcs per pack Accessories for microhematocrite capillary tubes Part no Descriptio...

Страница 46: ...afical Representation of the Rotors The graphical representation of the rotors shows the maximum and minimum radii of the accessories used If necessary the values must be manually calculated see 11 3...

Страница 47: ...the equation If then the speed or the rotation radius is changed the resulting RCF will be recalculated If the RCF is altered the speed will be adapted accordingly under the consideration of the radiu...

Страница 48: ...1 14K Appendix 48 Version 05 2010 Rev 1 8 of 01 09 2011 sb Translation of the original operating manual Fig 11 3 Speed Grravitational Field Diagram...

Страница 49: ...1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 9100 15 000 4 15C 4K15C 4 16 4 16K 6 15 6K15 6 16 6 16K without engraving only spincontrol professiona...

Страница 50: ...O 100 3 4 4 1 4 4 2 1 1 Antimony trichloride SbCl3 90 1 4 1 4 1 1 1 4 Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1 Benzene C6H6 100 3 2 4 1 3 4 4 1 1 Boric acid H3BO3 aqueous 1 3 1 2 1 1 1 Butyl acrylat...

Страница 51: ...rogen chloride HCl 5 1 4 1 4 1 1 1 1 4 Hydrogen chloride HCl concentrat ed 1 4 4 4 1 1 2 3 1 4 Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 1 3 Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 1 3 Hydrogen sulfide H...

Страница 52: ...rate AgNO3 100 1 1 1 1 1 1 1 1 1 4 Sodium bisulfite NaHSO3 10 1 1 2 4 1 1 1 Sodium carbonate Na2CO3 10 1 1 1 1 1 1 3 Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1...

Страница 53: ...EC Declaration of Conformity...

Страница 54: ......

Страница 55: ...y 23 26 Centrifuges definition 9 Changes in color 39 Changing the runtime during centrifugation 28 Changing the speed RCF value during centrifugation 27 Chemical and biological safety 16 Chemical reac...

Страница 56: ...unctions 32 Grafical representation of the rotors 46 Gravitational field 24 47 Grease for load bearing bolts 36 Ground wire check 18 Hazard warnings 9 24 Imbalance 17 25 Imbalance error 34 Importance...

Страница 57: ...11 Notes on safety and hazards 9 Notes on transport 20 Online download of forms 41 Opening the lid 23 Operating panel 26 Operating personnel 14 Operating voltage 22 Operation 26 Operational safety 37...

Страница 58: ...number 11 35 40 Service 40 Service contact 35 Service life 36 Service life of rotors and accessories 17 18 49 Service life of the accessories 39 Service request 3 35 40 Service work 40 Set up and conn...

Страница 59: ...erature error 34 Toxic substances 16 36 37 Transport safety device 21 Tubes 25 Type 43 Type name 11 Type of the centrifuge 35 40 Unpacking the centrifuge 21 Using the centrifuge 23 UV radiation 21 36...

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