Sigma 1-14, Руководство по эксплуатации

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1-14K
Using the Centrifuge
24 Version 05/2010, Rev. 1.8 of 01/09/2011- sb
Translation of the original operating manual
•
Open the centrifuge lid by pressing the lid key.
•
Unscrew the rotor fastening nut from the motor shaft (counter-
clockwise).
•
Lower the rotor with its central bore straight down onto the motor
shaft.
•
Hand-tighten the rotor fastening nut clockwise (corresponds to 1
Nm). The rotor fastening nut and conical spring washer must face
the rotor (i.e. the writing must be shown on top).
After frequent use, the rotor tie-down screw must be loosened by
some turns, the rotor has to be lifted and fastened again. This must be
done once a day or after 20 cycles. This ensures a proper connection
between the rotor and the motor shaft.
•
Rotors can be used without a cover. This leads, however, to
higher levels of noise and temperatures when running.
•
Follow the safety instructions and hazard warnings in chapter 3!
6.2.2.2 Installation of the Microhematocrite Rotor
•
Unscrew the rotor fastening nut from the motor shaft (anti-
clockwise).
•
Lower the microhematocrite rotor with its central bore straight
down onto the motor shaft.
•
Hand-tighten the rotor fastening nut clockwise (corresponds to 1
Nm). The rotor fastening nut and conical spring washer must face
the microhematocrite rotor (i.e. the writing must be shown on top).
Operation
•
Fill the capillary tubes with blood and seal them at one end with
putty or by fusion.
•
Place the capillary tubes into the recesses of the rotor with the
sealed end against the rubber ring. Ensure that the capillary
tubes fit tightly against the rubber ring. The opposite places must
be loaded.
•
Put the rotor cover on and lock it.
•
Close the centrifuge lid.
•
Enter the following parameters: speed 13,000 rpm, gravitational
field RCF max. 11,903 x g, time approx. 5 min.
•
Start the centrifuge.
•
Open the centrifuge lid when the rotor has stopped.
•
Unlock the rotor cover and lift it off.
Evaluation
•
Put the reader onto the rotor.
•
Turn the reader and perform a fine adjustment with the aid of the
central eccentric mechanism in order to localize the O-point and
the maximum liquid point in the capillary tubes. You can now
read the percentage value.
•
Remove the capillary tubes. Some of the tubes can also be
evaluated with the reader outside of the rotor (see the
instructions for use on the back of the reader).