ver.220216E
SHOWA DENKO K.K. (https://www.shodex.com/)
2
4.
Usable Conditions
Usable solvents are listed below.
(1) The standard eluents are buffers and aqueous solutions of salt such as sodium chloride, potassium chloride,
sodium sulfate, and potassium sulfate. Salts may also be added to the buffers. The recommended total salt
concentration range is 20 - 600 mM. Select a buffer which has positive charge ion and has a large buffering
capacity at the set pH.
pH Range
Buffer
4.8 - 5.0
N-Methylpiperazine·HCl
5.0 - 6.0
Piperazine·HCl
5.5 - 7.3
Bis-Tris
*1
·HCl
6.4 - 7.3
Bis-Tris Propane
*2
·HCl
7.3 - 7.7
Triethanolamine·HCl
7.5 - 8.0
Tris·HCl
8.0 - 8.5
N-Methyldiethanolamine·HCl
8.4 - 8.8
Diethanolamine·HCl
8.5 - 9.0
1,3-Diaminopropane·HCl
9.0 - 9.5
Ethanolamine·HCl
*1
2-bis(2-hydroxyethyl amino-2-(hyroxymethyl)-13-propanediol)
*2
1,3-bis(tris(hydroxymethyl)methylamino)propane
(2) A mixture of buffer (or aqueous salt solution) and methanol or acetonitrile of any ratio can be used. Up to
100 % methanol or acetonitrile can be used.
Attention!
· Use the column within above stated flow rate, pressure, and temperature ranges. Using
the column outside the given range may damage the column and lower its performance.
· When using a mixture of buffer or salt solution and organic solvent, make sure there is no
precipitation of salt.
· When using highly corrosive salts such as sodium chloride, wash out the salts at the end
of analysis. The metal parts of the devices and/or the columns may rust.
·
Column pressure is influenced by the eluent composition, flow rate, and column
temperature. When changing the eluent compositions, adjust the flow rate and column
temperature so that the column pressure remains below the usable maximum pressure.
5. Eluent Preparation
(1) Degas the eluent fully to prevent the formation of air bubbles.
(2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear
as noise on the chromatograms. Filter the eluent with a 0.45-
μ
m disposable filter to prevent the problems.
Attention!
· Whenever water is required, use ultra-pure water freshly generated by a water purification
system or water from a newly opened HPLC grade distilled water bottle. Use HPLC grade
organic solvent whenever possible. Solvents left in an opened bottle for a long time should
not be used. The content may have been changed, absorbed moisture, or has been
contaminated.
· Always use freshly prepared solvents. Solvents stored for a long time may have changed
their compositions and may influence elution patterns and/or damage the column.
Note
· Use of on-line degasser is recommended.
Product Name
Flow Rate (mL/min)
Maximum
Pressure (MPa)
(Per Column)
pH
Range
Temperature
Range (°C)
Recommended Maximum
Asahipak ES-502N 7C
1.0
1.5
1.2
2 - 12
10 - 50
