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HPLC Columns

MANUAL

Asahipak ODP-50

(C8P, C4P series)

Shodex HPLC Columns

Europe, Middle East, Africa, Russia

SHOWA DENKO EUROPE GmbH

Shodex Business

Konrad-Zuse-Platz 3

81829 Munich, Germany

E-mail:

[email protected]

Phone:

+49 (0)89 93 99 62 37

www.shodex.de

For technical support please use

contact details shown below:

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Содержание Asahipak C4P series

Страница 1: ...dex HPLC Columns Europe Middle East Africa Russia SHOWA DENKO EUROPE GmbH Shodex Business Konrad Zuse Platz 3 81829 Munich Germany E mail support shodex de Phone 49 0 89 93 99 62 37 www shodex de For technical support please use contact details shown below ...

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Страница 3: ...pak ODP columns are compatible with the commonly used reversed phase HPLC eluents between pH 2 to pH 13 The particles are available in two sizes The smallest has a diameter of five micron These are identified by the suffix 50 in the first column of Table 1 The small particles give the highest column efficiency and resolution but at the expense of higher operating pressure The Asahipak 90 series de...

Страница 4: ...nstructed from passivated 316 stainless steel The column end fitting is designed to minimize dead volume and band broadening The depth of the fitting for the inlet and outlet tubes may be different than those used on columns from some other manufacturers If you use a tube with a short nose extending from the fixed ferrule there will be a cavity in the flow path that can lower the column efficiency...

Страница 5: ...3 0 C8P 50 4E 4 6 x 250 5 10 11000 0 5 0 8 1 0 4D 4 6 x 150 5 10 7000 0 5 0 8 1 0 10E 10 0 x 250 5 10 8000 1 5 2 0 3 0 C4P 50 4E 4 6 x 250 5 6 9000 0 5 0 8 1 0 4D 4 6 x 150 5 6 6000 0 5 0 8 1 0 10E 10 0 x 250 5 6 7000 1 5 2 0 3 0 ODP 90 20F 20 x 300 9 17 9000 5 10 12 Table 2 Guard columns Grade Column Dimensions IDxL mm Columns guarded IDxL mm ODP 50G 4A 4 6 x 10 ODP 50 4 6x150 4 6x250 ODP 50G 6A ...

Страница 6: ...INSPECTION DATA provided with each column If the column is efficient then one can restore the column to operation in the instrument in the normal manner See also Section 5 1 on filtering and guard columns 4 2 Connection tubes For columns with diameters smaller than 10 mm id use connection tubes from the injector and to the detector that have the shortest practical length and internal diameter no l...

Страница 7: ... purpose Filtering is not always effective in preventing plugging of the column Experience shows that if column plugging is a frequent problem that the plug is often located at the very top of the column Guard columns are short sacrificial columns that are installed ahead of the main column to protect it When a plug occurs the guard column is replaced 5 2 Degassing Numerous practical problems are ...

Страница 8: ...ure low below 15MPa It is important to check the solubility of mixed solvent buffers before running a solvent program This can be done by making a quick scouting run in a test tube Slowly add the strong eluent to a small quantity of the starting eluent If the solution turns cloudy or white this means a precipitate is forming Other buffer combinations should be tried 7 Elution modes All Asahipak co...

Страница 9: ... degassing may be required to prevent formation of bubbles in the detector At low temperature the viscosity of the mobile phase will increase Care should be taken to avoid exceeding the maximum pressure specification of the column This may require decreasing the flow rate Flow rate is proportional to pressure so cutting the flow in half will decrease the pressure on the column by half 10 Column ha...

Страница 10: ... the initial mobile phase composition In some cases it is desirable to prepare the sample in a solution with an elution strength slightly weaker than the mobile phase Upon injection the sample components will tend to focus in a narrow band on top of the column This can be improve detection limits 12 Measurement of NTP The measurement conditions employed in the determination of NTP are described in...

Страница 11: ...nt is granted or implied by our sales of any material Shodex Asahipak products in this document are for laboratory use only They are not intended for medicinal or food use We assume no responsibility if these products are used for medicinal or food purposes or are misused in any way Shodex Asahipak products are not intended for clinical use While clinical applications may be shown these products a...

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