Next, you need to fill in the information in the Standard Table, and the Sample Table.
The Standard table needs concentration information filled in, otherwise the Read
Standard button will be grayed out.
»
Be sure to enter the concentrations of
your standard solutions. Otherwise, the
Read Standard Button will not be active.
½
Be sure to enter sample names, otherwise the
Read Sample Button will not be active.
It is a good practice to click on the Baseline button, with nothing in either the
reference or the sample side of the compartment. (Be sure that the sample lid is
closed). This will set the absorbance to zero at every wavelength.
Then, put cuvettes with blank solution in both the sample and reference sides, and
click on Cell Blank (with the lid closed). This will read the absorbance of the blank
cells. This blank absorbance reading will be subtracted from the absorbance
readings of all standards and samples measured after it.
After your data is collected, you must save the data by doing a File, Save As.
