Seahorse XF24, Руководство по обучению

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Basic Procedure
7
XF24 Analyzer Materials List
Materials required or recommended for a successful XF24 assay.
Required
The following are the materials required to run an XF24 assay.
•
XF24 Analyzer with Controller
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Non-CO
2
Incubator (37°C) or XF Prep Station
•
XF24 FluxPak (Part # 100850-001 ) or XF24 FluxPak mini (Part # 100867-100)
•
pH Meter
•
Inverted Phase Contrast Microscope
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Pipette
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P20 and P1000 Pipette tips
•
Low-buffered non-bicarbonated Medium for Assay
(e.g. DMEM, KHB, RPMI without bicarbonate)
Recommended
The following are materials recommended to help you achieve optimal results. These include ensuring
optimal instrument functioning, compound reconstitution, and assay experience.
•
Uninterrupted power source
•
Internet access
•
Balance
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Stir plate
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Cell counter/Hemacytometer
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Water bath set at 37°C
•
Micro centrifuge
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Touch vortex
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Centrifuge with adaptors for cell culture plates
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Pipetting Reservoirs
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15 and 50 mL conical tubes
•
Sterile filter bottles (0.22 uM filter) and cap