4.2 Loading start
Push approximately 5 ml of protein
solvent through the unit at any
speed. If you detect air bubbles just
lift plunger a few mm to let it ascend
into the syringe.
4.2 Loading end
The red protein is bound on the
membrane and the contaminant
Amido Black flows through.
4.3 Washing
Wash with 5 –10 ml of equilibration
buffer.
4.4 Elution
Elute with 3 ml elution buffer.
The unit may not run dry during the
operation.
The red protein is eluted and concen-
trated at the same time.
4.5 Regeneration
Regenerate the unit by application of
10 ml of equilibration buffer.
4.6 Storage after use
Keep the used unit filled with equili-
bration buffer in presence of an
antimicrobial preservative such as
20 % ethanol.
4.7 Stability
The membranes are stable in a pH
range of 2–12 (long term) and 1–14
(short term). Do not use oxidative
agents, such as hypochlorite or H
2
O
2
.
4.8 Use of peristaltic pumps or
liquid chromatography systems
For the operation of Membrane
Adsorber units with a peristaltic
pump or a LC system, specific Luer
Lock adapters are needed (see Order-
ing Information). Proceed as
described under 4.1 Equilibration.
When the unit is filled completely
with equilibration buffer, close the
outlet and remove the syringe. Start
your LC system or peristaltic pump
at a very low flow rate. When fluid
emerges, stop the pump, connect the
tubing via a Luer Lock adapter to the
inlet of the unit. Make sure that no
air is introduced. Remove the lower
cap. Run the pump until fluid
emerges from the outlet of the unit
and stop it. Then connect outlet of
the unit via Luer Lock adapter to the
LC detector and proceed with load-
ing.
Important note
For in vitro use only.
Use the units only as described in this
operating instructions.
Follow safety regulations: Wear
protective gloves, safety glasses and
a lab coat during work.
Related products for
laboratory scale
Order number
Description
S5f
Sartobind S 5,
5 cm
2
membrane
area, single layer,
15 pcs. Single use
S15X
Sartobind S 15,
15 cm
2
, 3 layers,
2 pcs., reusable
S75X
Sartobind S 75,
75 cm
2
, 15 layers,
2 pcs., reusable
S100X
Sartobind S 75,
100 cm
2
, 5 layers,
2 pcs., reusable
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
37079 Goettingen, Germany
Phone +49.551.308.0
Fax +49.551.308.3289
www.sartorius-stedim.com
USA Toll-Free +1.800.368.7178
UK +44.1372.737159
33.442.845600
Italy +39.055.63.40.41
Spain +34.91.3586102
Japan +81.3.3740.5407
Sartobind
®
is a trademark of
Sartorius Stedim Biotech GmbH,
Goettingen, Germany.
Technical data subject to change
without notice.
Printed and copyrighted
by Sartorius · W404.18
Publication No.: SL-6139-e08041
