Sartorius Stedim Biotech Sartocon, Руководство пользователя, Страница: 14 / 60

14 Start up of cassettes
5.4.1 Manual Integrity Test
Proceed as follows:
t
Attach a hose to the feed inlet (V
1
), and connect the other end of the hose to a
compressed air source.
t
Close the retentate outlet (V
2
).
t
Adjust the inlet test pressure pinlet to the value specified [ Æ Chapter “6. Technical
Data”, page 26], and keep this pressure constant.
t
Close the lower permeate outlet valve V
3
.
t
At a constant inlet pressure pfeed, wait four minutes until the system has stabilized.
t
Use a flow meter or an inverted burette filled with water to measure the air
diffusion at the upper permeate outlet.
V
4
Retentate valve
outlet closed
Compressed air
Feed
Inlet
V
2
P
2
P
1
P
3
V
3
Permeate
closed
Cassette
V
1
Permeate
open
Fig. 1: System set up for manual integrity testing
5.4.2 Automatic Integrity Test (Pressure Decay)
Proceed as follows:
t
Connect the integrity tester [such as Sartocheck ® 3 plus (16290) or S
artocheck ® 4 Plus (26288)] to the power supply and pressure source.
t
Close the retentate outlet (V
2
) and open the permeate outlets (V
3
, V
4
).
Open V
5
and close V
1
.
t
Enter the required test parameter according to the Sartocheck ® instruction manual
and cassette data.
t
Use the following parameter:
stab. time: 4 min.
test time: 2 min.
t
Enter the maximum allowable diffusion value [ Æ Chapter “6. Technical Data”,
page 26].
t
Start the Integrity Test.
Sartocheck ®
Retentate outlet
closed
V
5
V
2
Pump
V
1
V
4
V
3
Feed inlet
closed
Permeate open
Permeate open
Cassette
P
1
P
2
P
3
Fig. 2: System set up for automatic integrity testing. V
1
–V
5
: valves; P
1
–P
3
: pressure gauge.
If the hold up volume exceeds 9 l, please contact your Sartorius Stedim application
specialist.