Connection of CO
2
gas cylinder
WARNING
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of CO
2
gas.
Elevated level of CO
2
gas can be hazardous to health and may
lead to asphyxiation and risk of death.
1.
Use a liquefied CO
2
gas cylinder, not a siphon (dip tube) type. The CO
2
gas should be 99.5% or more
pure.
2.
Install a gas pressure regulator (optional accessory MCO-100L) on the cylinder. Use a regulator
rated at 25 MPaG (250 kgf/cm
2
G, 3600 psiG) on the primary side and 0.2 MPaG (2.0 kgf/cm
2
G, 30 psiG)
on the secondary side.
3.
Using the gas supply pipe provided, connect the CO
2
gas pressure regulator to the connecting port for
CO
2
gas pipe located at the rear left side of the CO
2
incubator.
Note:
If CO
2
is supplied to multiple CO
2
incubators from a single gas cylinder, a CO
2
solid will be formed in the
gas pressure regulator. The gas pressure regulator safety valve will operate, and there may an explosive
sound.
When the MCO-21GC is not mounted
Using the gas supply pipe that is provided, connect the CO
2
gas pressure regulator for the CO
2
gas
cylinder to CO
2
gas pipe connection port A on the incubator. After connecting the pipe, check to make
sure that no gas is leaking.
When the MCO-21GC is mounted
Connect a pair of CO
2
gas cylinders with CO
2
gas pressure regulators independently. The gas supply line
will be switched automatically. Connect the main cylinder to port A and the reserve cylinder to port B. After
connecting the cylinders, check to make sure that no gas is leaking.
4.
Set the CO
2
pressure on the secondary side to 0.03 MPaG (0.3 kgf/cm
2
G, 4.3 psiG) (at gas injection).
Excessive pressure may cause disconnection of internal pipes inside the CO
2
incubator which will result
in leakage of CO
2
gas into the atmosphere.
Elevated level of CO
2
gas can be hazardous to health
and may lead to asphyxiation and risk of death.
The repair of the incubator will be necessary if the
internal pipe is disconnected.
4.
Check that no gas is leaking at any point where the pipe connects with the CO
2
regulator or the CO
2
incubator.
Note:
The incubator, including the gas supply pipes and services must be examined at frequent intervals to
ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.
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