Ryf Axio Lab.A1, Руководство по эксплуатации

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OPERATION
Carl Zeiss Lighting and contrasting method in transmitted light Axio Lab.A1
84 430037-7144-001
04/2013
x
Limit to aperture to a value of about 0.2.
x
Turn the rotary stage Pol until the specimen is almost obliterated, i.e. completely dark , and set the
45° locking position.
x
Rotate the stage once (by 45°) so that the specimen is in a diagonal position (bright).
The interference intensity or color leads to the following conclusion:
If more or less strong interference colors appear on the specimen, the path difference ranges
approximately between 1/2
O
and 5
O
.
The suitable compensator is:
Tilting compensator B 0-5
OO
.
If the specimen-side color changes from light gray/white to a strong interference color, when a
compensator
O
(473704-0000-000) is inserted in the compensator slot, the path difference is
(1/4 ...1/2)
O
.
A prerequisite for occurrence of the color change effect may be the evaluation in two specimen
positions rotated at an angle of 90° from one another, plus a centered stage.
The suitable compensator is:
Tilting compensator B 0-5
O
or the compensation method according to DE SENARMONT up to 1
O
using the Senarmont compensator 546/4 nm .
The compensation method according to DE SENARMONT requires the use of the rotatable
analyzer.
After insertion of the compensator
O
and rotation of the object by 90°, the interference color remains
white; in this case, however, it is a "higher-order white" and thus the path difference is > 5
O
.
The suitable compensator is:
Tilting compensator K 0-30
O
(Accessory 000000-1115-698)
A dark gray as appearing interference color suggests very small path differences (
O
/10 or 54.6 nm).
The suitable compensator is:
Brace– Köhler rotary compensator
O
/10 (Accessory 000000-1115-703).
x
Insert the compensator into the slit as far as it will go.
The accompanying instructions must be observed for measurement preparation and procedure.