E. Operating Procedure
1.
BioVac 225 <pic 1>
a.
Ensure that the overflow protection is installed properly then place the suction bottle on the holder.
Please refer to <pic 9> when installing it.
Note: please always put the overflow protection into the waste bottle cover gently to avoid
the deformation of its plastic tube that could cause a malfunction.
b.
Connect disc filter with tubes according to <pic 4>.
c.
Connect the tube connecting adaptor to both thin, thick silicone tube and suction bottle as <pic 4>
then select a desired fitting part to be connected with the thin silicone tube.
d.
Plug the power cord into a proper power supply then press power switch to turn on the machine.
After blocking the fitting part with a hand, adjust vacuum level to a desired value by turning the
vacuum regulator then proceed with suction operation.
e.
The 1-channel tip adaptor (150mm) is recommended to be plugged into the handle then have it
connect with 200 µl disposable tip (the other side can connect with 1µl disposable tip ) with
which to prevent cross contamination in common microplate and culture dish.
f.
The complete set of BioDolphin suction kit (199100-00) can cater for various kinds of waste liquid
container and suction.
g.
After the process is finished then turn off the machine. Dispose of the contents in the waste bottle
in accordance with the applicable regulations.
h.
Proceed with cleaning and maintenance after every use.
Note: Check the liquid level of the waste bottle before each use and then empty it as per the
applicable regulations.
2.
BioVac 225 Plus <pic 2>
a.
Ensure that the overflow protection is installed properly then place the suction bottle on the holder.
Please refer to <pic 9> when installing it.
Note: please always put the overflow protection into the suction bottle cover gently to avoid
the deformation of its plastic tube that could cause a malfunction.
b.
Install the complete system according to <pic 5>.
c.
Plug the power cord into a proper power supply then press power switch to turn on the machine.
After blocking the handle with a hand, adjust vacuum level to a desired value by turning the
vacuum regulator then proceed with suction operation.
d.
Ensure that air comes into the front port once pressing the suction button on the handle before
proceeding with suction operation.
e.
The 1-channel S.S. adaptor (40 mm) can be plugged into handle directly for waste suction of
microplate or culture dish by pressing the suction button or pressing lock button to keep a
continuous operation.
f.
The 1-channel tip adaptor is recommended to be plugged into the handle then have it connect
with 200 µl disposable tip (the other side can connect with 1µl disposable tip ) with which to
prevent cross contamination in common microplate and culture dish.
g.
For 96-well microplate ELISA test, the 8-channel tip connector is recommended to be plugged into
