Published by
Roche Diagnostics GmbH
Roche Applied Science
68298 Mannheim
Germany
©
2009 Roche Diagnostics
All rights reserved.
���
120
9
Measuring beads used by the 454 Sequencing System.
6. Setup
selection
Load the appropriate setup in the Setup selection list
(as described in 4.) or if not already available create and save
a setup for the beads (as described in 4.) with the following
parameters:
SETUP NUMBER: 01
NAME:
Beads
Capillary: 150
μ
m
Sample Volume:
3 x 400
μ
l
Dilution:
3.333e+03
Left Cursor:
15.00
μ
m
Right Cursor:
25.00
μ
m
Display Mode:
Standard
Total Counts/ml:
Off
Interface:
Parallel
Print-Mode:
Manual/PF On
USE MENU AND NEXT TO STEP. USE +/- TO EDIT. USE
CANCEL TO LEAVE SCREEN OR ENTER TO CONFIRM CHANGES
7. Preparing
samples.
Rotate the recess in the sample platform until it points
forwards and remove the CASY
cup from below the
measuring capillary.
Place the sample to be measured below the measuring capillary
and ensure that the external platinum electrode is immersed
in the sample.
Rotate the sample platform further to the side.
Press
START
to perform a measurement using the current
parameters. CASY Model DT automatically works through all
the necessary steps. After a short time, the results for your
sample appears in the display.
Since the beads used by the 454 Sequencing System are in the
size range between 15 and 25 µm the parameter
Counts/ml
15.0 – 25.0 µm:
1,140e+07 indicates the measured bead
concentration per ml in the bead suspension to be examined
(used in 7.).
9.
Changing samples and fi nishing measurements
Provided you measure samples of the same type in a similar
concentration range, you do not necessarily have to fl ush
the system when samples are changed. If you expect larger
concentration differences or wish to perform measurements
with different sample types, before a new measurement, place
a CASY
cup containing freshly fi ltered CASY
ton below the
measuring capillary and fl ush the system using the
CLEAN
function.
Before switching off the CASY Model DT monitor again the
background (see 5.).
SET:
Beads-01
SN:
DT-2XX-9999
CAP:
150
μ
m
DATE: 15.09.2009
SVOL: 3x 400
μ
l
L.CURSOR:
15.0
μ
m
TIME: 10:15
DIL:
3.333E+03
R.CURSOR:
25.0
μ
m
Conc. Range:
OK
Counts:
4533
Counts/ml
3.3-15.0
μ
m:
1.127e+06
Counts/ml
15.0-25.0
μ
m:
1.140e+07
Counts/ml
25.0-100.0
μ
m:
3.684e+05
For life science research only. Not for use in diagnostic procedures.
CASY and 454 are trademarks of Roche.
Fill a CASY
cup with 10 ml CASY
ton (for highest accuracy
ensure that exactly 10 ml CASY
ton are pipetted into the
CASY
cup).
Add 3 µl of the homogenized bead suspension to be examined
(for highest accuracy ensure that exactly 3 µl are pipetted into
the 10 ml CASY
ton).
Secure the lid and mix the sample by tilting the CASY
cup three times. Carefully avoid the formation of bubbles or
foam!
8.
Measuring samples and displaying the measuring results
25381209 CASY Counter Model DT-Operating Instructions.indd 4
25381209 CASY Counter Model DT-Operating Instructions.indd 4
18.12.2009 16:47:16
18.12.2009 16:47:16
