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3
GETTING STARTED
2. Placing the Specimen Slide
Push the silver finger of the specimen holder away from you (1). Place the
specimen slide (2) against the other side of the specimen holder while holding the
finger open. Once slide is placed properly with the cover glass facing upward you can
release the silver finger so that the slide remains clamped. Use the x and y axis adjust
ment knobs (3) to manuever the slide into the proper position where it is centered
above
the
light
source.
3.
Adjust Focusing
a.
Shift the 4x objective into the light path until you hear it click properly in place.
b.
Observe with just the right eye in the right eyepiece. Rotate the coarse focusing knob until the
image appears clearly in the field of view.
c.
Rotate the fine focus knob to achieve finer focused details.
2.
Coarse Focus Tension Adjustment
To increase the tension, turn the tension adjustment ring located on the inside of the coarse focus knob
in the direction indicated by the arrow.
3.
Interpupillary Distance Adjustment
Before adjusting the interpupillary distance, bring a specimen into
focus using the 4x objective. While looking through the eyepieces
move the eyetubes either further apart or closer together until the
right and left field of view become one. Line up the dots (1) on either
side of the basepoint scale (2). This adjustment will enable the user
to see the specimen with both eyes.
4.
Diopter adjustment
Diopter adjustment will compensate for the differences in vision between the left and right eyes. In addition
to making observation with both eyes easier, it will also help reduce the extent to which focusing is lost when
the objective magnification is changed. In particular, this occurs when a low magnification objective is used.
Rotate the nosepiece until the 4x objective “clicks” into position. Using the fine and/or coarse focus
knobs, focus the microscope until the image is at its sharpest. The left and right eyepieces have
separate focusing provisions to compensate for slight differences in the focusing of each eye.
Using the right eye only and viewing through the right-hand
eyepiece, adjust the diopter until the image is clearly in focus.
Using the left eye only and viewing through the left-hand
eyepiece with its independent diopter focusing ring, focus
until the specimen is at its sharpest. Once completing these
steps, the microscope should now be ready for binocular viewing.