2
2. Place standard specimen slide (cover slip up) on top of stage surface.
a. Swing moveable finger on slide holder outward. Place specimen slide against fixed side of slide
holder. Slowly release moveable finger until it makes contact with the specimen slide.
3. Rotate coarse focusing controls until the specimen comes into focus.
4. Adjust fine focus controls until specimen is in sharp focus.
5. Adjust diopter for difference in eyesight.
a. Using right eye, peer into the right eyepiece tube. Adjust sharpness of image by turning diopter
adjustment located on left eyepiece tube.
6. Adjust the aperture (opening) of the iris diaphragm.
Iris diaphragm should not be used to control the brightness of illumination, use light intensity control
knob to adjust light level. Iris diaphragms are designed to help achieve high resolution of specimen and
provide contrast in the image. Smaller apertures will deliver contrast to the image. However, closing
aperture too much will reduce resolution. Experimentation is the best method of determining the
correct opening of the diaphragm. Some suggested openings for the iris diaphragm are:
7. Changing magnification.
a. Rotate revolving nosepiece to postition 10x objective into optical path.
b. This microscope has been parfocalized, which allows changes from one objective to another
while requiring only a slight adjustment of the fine focus controls.
c. When changing to the 40x and 100x objective lens, care must be exercised in order to prevent
damaging the front lens element and specimen slide.
d. In order to obtain the maximum resolution of the 100x oil immersion lens, it is necessary to
apply immersion oil between the cover glass of slide and the front lens of the objective.
1.) Use a very small amount of immersion oil. Only the tip of the lens should ever come in
contact with the oil.
Objective
4x
10x
40x
100x
Diaphragm Opening
1/8 open
1/8 to 1/4 open
1/4 to 1/2 open
1/2 to 3/4 open
