KrosFlo® TFDF™ Lab System
User Guide
48
repligen.com IF.UG.023 R1
Figure 37. Concentration/Diafiltration/Concentration Mode Run Setpoints screen
Commands:
•
Start:
Starts the automated run at the setpoints entered
•
Stop:
Stops the run
•
Enter:
This button appears on both the Overview Screen and the Run Setpoints screen once
the mode is doneselect the
Enter
button to acknowledge the automation mode is done
Initial Concentration setpoint values (all modes):
•
Target Speed of Feed pump (P-01):
The target speed of the magnetic levitating
recirculation/feed pump controls the rpm of the pump with feedback from clamp-on flow
meter (FL-01)
•
Target Speed of Permeate pump (P-02):
Input the target speed of the permeate pump in
either ml/min or VVD, the system measures the change in weight on the permeate scale and
controls the rpm of the permeate pump to match target permeate rate
•
Initial Concentration SP (CF1):
A dimensionless concentration factor used to quantify
concentration of the feedstock it is the amount that the feedstock has been reduced in
volume relative to the initial volume for example, if 1 L of feedstock is concentrated to 0.25L
with 0.75 L padding through the filter as permeate, a 4-fold concentration has been
performed, the Concentration Factor would therefore be 4
•
Permeate Weight:
This value refers to the total cumulative permeate weight at end of the
final concentration step, input the target weight for the cells mass on the WE-01 scale, the
system controls the rpm of P-03 to keep the mass at the target weight, tare with the empty
product vessel so only the weight of the sample is read, the target should then be the weight
of the sample once the recirculation line is filled
Diafiltration setpoint values (Concentration/Diafiltration and Concentration/Diafiltration/
Concentration modes):
