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Hum Bug Reference Manual    Page 3 

 

Quest Scientific 

INTRODUCTION 

This manual describes the operation, connections and normal use of the Hum Bug in the 

neurosciences  and  related  fields  of  research.    The  original  Hum Bug  was  developed  in  the 
electrophysiology  laboratory  of  Dr. Tom  Richardson  in  response  to  problems  with  electrical 
interference during intracellular studies.  In theory, proper attention to grounding and appropriate 
shielding can eliminate 50/60

1

 Hz noise induced by electrical wiring, power supplies, and lights.  

In practice, this noise remains a frequent and distressing problem in the daily operation of many 
electrophysiology laboratories.  Even with diligent attention to detail, noise may come and go for 
no apparent reason and may appear during critical phases of data collection.  The effort required 
to maintain noise at an acceptable level is time consuming and frustrating. 

Notch

  or 

Comb

  filters  are  sometimes  used  to  attenuate  50/60  Hz  noise  and  related 

harmonics.    Unfortunately,  the  composition  of  many  biological  signals  includes  these 
frequencies.  As a result, critical components of the signal are filtered along with the noise.  This 
distortion and loss of information is usually unacceptable for scientific investigation. 

The  Hum Bug  is  a  simple  and  hassle  free  solution  to  these  problems.    It  will  eliminate 

electrical  interference  from  physiological  recordings  without  altering  your  signal  of  interest.  
Simply insert it into the signal path of your recording system and it will automatically eliminate 
noise without any need for adjustment. 

The  Hum Bug  is  not  a  filter.  It is a new 

class

 of instrument capable of eliminating 

electrical  interference  from  analogue  signals  without  creating  phase  shifts,  frequency  loss, 
amplitude errors, DC shifts, time delays, or digital distortion.  It effectively eliminates 50/60 Hz

2

 

noise  and  harmonics

3

  without  altering  the  frequency  characteristics  of  the  desired  signal

4

  even 

when frequencies within the signal overlap with noise components.  It uses an advanced signal 
processing circuit to continuously construct a replica of noise present on the input and to subtract 
this  replica  from  the  original  signal.    The  end  result  is  a  clean  signal  which  is  free  from 
distortion.  It performs this function in the presence of biological activity even when the shape, 
amplitude, and harmonic content of the noise evolves over time. 

The  Hum Bug  can  eliminate  50/60  Hz  noise  from  virtually  any  analogue  signal.    It  is 

equally  effective  at  removing  noise  associated  with  inadequate  grounding,  ground  loops,  and 
electrical  pickup.    Common  applications  include  noise  elimination  from  signals  recorded  using 
microelectrodes, skin electrodes (EKG, EMG, EEG), high gain amplifiers, magnetic sensors, and 
audio equipment. 

                                                 

1

  The frequency depends on the AC frequency of the power mains.  This frequency is 60 Hz in North America and 

50 Hz in many other regions of the world. 

2

 Each Hum Bug is internally configured for either a 50 or 60 Hz fundamental. 

3

  Electrical  interference  is  usually  composed  of  a  variety  of  frequencies  which  are  harmonics  of  the  50/60  Hz 

fundamental.    Frequencies  as  high  as  1 kHz  are  not  uncommon.    The  Hum Bug  will  eliminate  harmonics  with 
frequencies up to 4 kHz. 

4

 For frequencies up to at least 500 kHz  

Содержание Hum Bug

Страница 1: ...NTS Introduction 3 Theory Of Operation 4 Installation 6 Controls 7 Input Signal 7 Noise Generated By Monitors 8 Sources Of Complex Noise 9 Adaptation Rate 9 Front Panel 10 Back Panel 11 Trouble Shooting 12 Specifications 18 Warranty And Contacts 18 ...

Страница 2: ...Page 2 Hum Bug Reference Manual Quest Scientific ...

Страница 3: ...oise without any need for adjustment The Hum Bug is not a filter It is a new class of instrument capable of eliminating electrical interference from analogue signals without creating phase shifts frequency loss amplitude errors DC shifts time delays or digital distortion It effectively eliminates 50 60 Hz2 noise and harmonics3 without altering the frequency characteristics of the desired signal4 e...

Страница 4: ... 5 to 10 seconds under ideal conditions but is slower when low amplitude noise is dominated by ongoing physiological activity and or noise harmonics are greater than 1 kHz Therefore the noise replica may lag behind if the noise characteristics suddenly change Under these conditions some noise will transiently appear on the output while the Hum Bug adapts to the new characteristics The major advant...

Страница 5: ...Hum Bug Reference Manual Page 5 Quest Scientific ...

Страница 6: ... operating mode During your initial evaluation of the Hum Bug it is a good idea to turn off any computer monitors in the vicinity of your recording setup This will eliminate possible confusion between 50 60 Hz noise and any additional interference associated with your monitors See the section on monitor noise for more details After an initial warm up period of approximately thirty seconds the Hum ...

Страница 7: ... Pushing it will clear the noise replica and force the Hum Bug to generate a new noise replica from scratch In effect this causes the Hum Bug to immediately forget the noise waveform This function is seldom necessary since the Hum Bug automatically adapts to changing noise conditions INPUT SIGNAL The Hum Bug will operate on analogue signals originating from virtually any laboratory equipment inclu...

Страница 8: ...erference is the same as the power mains so it will be eliminated by the Hum Bug The second form of interference referred to as refresh noise is generated each time a monitor refreshes the image displayed on its screen The frequency of this interference will depend on the video mode assigned to the monitor In most cases it will differ from the power mains and will not be recognized as noise by the...

Страница 9: ...ncy response is acceptable will depend on your application Feel free to consult Quest Scientific for more information ADAPTATION RATE The Hum Bug adapts to changing noise characteristics in the input signal Under normal conditions these characteristics evolve slowly and the Hum Bug is able to maintain effective noise elimination However noise will appear in the output signal following a sudden cha...

Страница 10: ...lowly over time However touching a manipulator approaching the electrode or lifting the electrode from the experimental preparation can cause additional but transient noise on the recorded signal Using HOLD during these manipulations will keep the Hum Bug from chasing the transient noise As a result effective cancellation of ongoing noise can resume immediately after the manipulation is complete ...

Страница 11: ...Hum Bug Reference Manual Page 11 Quest Scientific BACK PANEL ...

Страница 12: ...t to the expected behavior described for each procedure Step One Set up your equipment in a manner similar to the illustration shown below The objective is to simultaneously view the input and output of the Hum Bug on different channels of an oscilloscope while recording a noisy signal This description assumes that you are recording neuronal activity using a micropipette but a similar procedure co...

Страница 13: ...ed as the noise replica grows to the required size When adaptation is complete the Indicator should be off Toggle BYPASS mode on red LED and off green LED to observe the signal with and without noise cancellation Compare the input and output signals You should find that the primary signal of interest is not modified by the Hum Bug Step Four Observe the Hum Bug adapt to changing noise conditions On...

Страница 14: ...us on different types of abnormal Hum Bug behavior The tasks outlined in each section are designed to help you identify the specific cause of abnormal behavior in your Hum Bug A The Hum Bug fails to operate 1 No LEDs are illuminated on the panel switches and the indicator does not illuminate when you push clear Check that the power switch is on Check that the power cable on the back panel is pushe...

Страница 15: ...ce Perhaps the peak to peak level of noise is too large for the Hum Bug to completely cancel If so the indicator will continue to flash red Connect the input signal of the Hum Bug to an oscilloscope and determine the noise amplitude The Hum Bug cannot cancel noise greater than 1 Volt peak to peak If your noise levels are higher than this limit you may have to reduce the gain in your preamplifier P...

Страница 16: ...put is grounded but much larger than expected when an input signal is connected to the Hum Bug Perhaps the grounding of the Hum Bug and associated equipment is the problem See section D1 Are you connecting the Hum Bug to an unusual piece of equipment If so check the output impedance of the device Although the Hum Bug will adapt to a wide range of input characteristics a signal source with a very h...

Страница 17: ...und wires will make inappropriate connections between grounds in your setup and the grounds in the power mains Check your cables to make sure that the braided shield is connected to the ground ring of the BNC connector on both ends If you have tried the suggestions outlined above and still have problems or questions please contact Quest Scientific ...

Страница 18: ... Hz to 4 kHz Controls BYPASS halts noise cancellation by routing input directly to output HOLD suspends adaptation to evolving noise characteristics CLEAR clears the noise replica Display LED indicates changing noise levels GREEN decreasing amplitude of the noise replica RED increasing amplitude of the noise replica QUEST SCIENTIFIC 1164 West 21st Street North Vancouver BC Canada V7P 2C8 Voice and...

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