Qiagen RNeasy Fibrous Tissue, Руководство пользователя

Страница: 11 / 38

11 RNeasy Fibrous Tissue Mini Handbook 01/2018
Table 1. RNeasy Mini spin column specifications
Specification RNeasy Mini spin column
Maximum binding capacity 100 µg RNA
Maximum loading volume 700 µl
Minimum elution volume 30 µl
Maximum amount of starting tissue ≤ 30 mg
Note
: If the binding capacity of the RNeasy Mini spin column is exceeded, RNA yields will
not be consistent and may be reduced. If lysis of the starting material is incomplete, RNA
yields will be lower than expected, even if the binding capacity of the RNeasy Mini spin
column is not exceeded.
Table 2. Typical yields of total RNA
Mouse/rat tissue (10 mg) Yield of total RNA (µg)*
Heart 8–12
Aorta 8–12
Trachea 8–12
Esophagus 8–12
Muscle 5–10
Skin 4–8
Eye 8–12
Ear 10–15
* Amounts can vary due to factors such as species and developmental stage. Since the RNeasy Fibrous Tissue Mini Kit
enriches for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA
and other low-molecular-weight RNAs, which make up 15–20% of total cellular RNA.