7.2
Fluorescein Calibration
The SP-380 is designed to provide a flexible calibration procedure. The user can start with the two-point
(DI water and 50 ppb fluorescein) calibration and progressively add a three- and four-point calibration with
250 ppb and 500 ppb fluorescein, respectively. This allows the user to choose a procedure based on the
need of measurement accuracy and the target fluorescein range.
*
NOTE
*
It is highly recommended to complete a full, four-point calibration for the best
accuracy across the entire fluorescein measurement range of the SP-380 (0–600 ppb).
Follow the steps below to conduct either a two-point, three-point, or four-point fluorescein calibration:
7.2.1
Two-Point Calibration
1. Rinse the sample cell three times with deionized (DI) water. Fill the sample cell with the DI water.
2. Power on the SP-380 by pressing
. Allow 5–10 seconds for the SP-380 to stabilize.
3. Press
FC
(
) to switch to Fluorescein measurement mode.
4. Press
to launch the
FLCN CALIBRATION
screen (Figure 7).
5. Press
Zero
(
) to start the zero (blank) calibration.
6. If the calibration succeeds, a checkmark (
) and instructions for the 50 ppb slope calibration will
appear (Figure 8).
7. Rinse the main module sample cell three times with 50 ppb Fluorescein standard. Fill the sample cell
with the standard.
8. Press
Slope
(
or
) to start 50 ppb slope calibration.
9. If the calibration succeeds, a second checkmark (
) ”
Calibration Success
” message will appear
(Figure 9). Otherwise, a warning message is displayed.
10. After a successful two-point calibration, choose one of two options:
(a) Press
Continue
(
or
) to proceed to a three- or four-point calibration,
or
(b) Press
Exit
(
) to end the calibration process at a two-point calibration and return to Fluores-
cein measurement mode.
SP-380 User Manual
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9
Содержание SP-380
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