8
4. Operation
MRJ
4-6 Center the condenser
(1)
Rotate the condenser up-down knob
①
to raise it to the
highest position.
(See fig.6)
①
②
③
(2)
Rotate the 4X objective into the light path.
(3)
Rotate the Aperture Diaphragm
②
in a counterclockwise
direction and move to the smallest setting. and you will see the
image of field diaphragm from the eyepieces.
(4)
You want to see the circle of light closest to the center. If
is centered go to section 4-7, if not go to the next step
it
.
Fig.6
Image of aperture diaphragm (inside)
(5)
Adjust the center adjustment screw
③
and put the light
to the center of the field of view [Make sure the aperture
diaphragm is
smaller than the field of view of the eyepiece
(See fig.7)
. When the shadow around the field of view of the
eyepiece is symmetrical, it shows the condenser has been
centered correctly.
(See fig.7)
(6)
Now, you can enlarge the aperture diaphragm
②
and
remove the shadow.
Fig 7
Outside
edge of
objective
4-7 Adjust aperture diaphragm
②
The aperture diaphragm decides the numerical aperture of
the
illumination. If the N.A. of illumination is matching with N.A.
of the objective
②
, you can obtain better resolution and the
contrast.
If the contrast is low, you can adjust the N.A. of the condenser
to 70%-80% of the N.A. of the objective when using. Adjust
the upper diaphragm ring until you see the image like above
(See fig.8).
Use of diaphragm and put the N.A. of condenser
Fig 8
②
to 80% of the objective. e.g.: objective 40X (N.A. =0.65),
put the aperture diaphragm to about 0.65X0.8=0.52.
(See
fig.9)
Fig.10
Switch to open and
close
Aperture
Diaphragm
Fig 9