Photometrics Cascade 128 Скачать руководство пользователя страница 5

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Cascade User Manual

 

Owner's Manual and Troubleshooting 

You should read the owner’s manual thoroughly before operating this product. In the unlikely event that you 

should encounter difficulty operating this product, the owner’s manual should be consulted before contacting 

the Photometrics technical support staff or authorized service representative for assistance. If you have 

consulted the owner's manual and the problem still persists, please contact the Photometrics technical support 
staff or our authorized service representative. 

See Item 12 in the following section of this warranty ("Your 

Responsibility") for more information. 

Your Responsibility 

The above Limited Warranties are subject to the following terms and conditions: 

1.

 

You must retain your bill of sale (invoice) and present it upon request for service and repairs or 

provide other proof of purchase satisfactory to Photometrics. 

2.

 

You must notify the Photometrics factory service center within  (30) days after you have taken 

delivery of a product or part that you believe to be defective. With the exception of customers who 

claim a “technical issue” with the operation of the product or part, all invoices must be paid in full in 

accordance with the terms of sale. Failure to pay invoices when due may result in the interruption 

and/or cancellation of your one (1) year limited warranty and/or any other warranty, expressed or 

implied.  

3.

 

All warranty service must be made by the Photometrics factory or, at our option, an authorized 

service center. 

4.

 

Before products or parts can be returned for service you must contact the Photometrics factory and 

receive a return authorization number (RMA). Products or parts returned for service without a return 

authorization evidenced by an RMA will be sent back freight collect. 

5.

 

These warranties are effective only if purchased from the Photometrics factory or one of our 

authorized manufacturer's representatives or distributors.  

6.

 

Unless specified in the original purchase agreement, Photometrics is not responsible for installation, 

setup, or disassembly at the customer’s location. 

7.

 

Warranties extend only to defects in materials or workmanship as limited above and do not extend to 
any product or part which has: 

 

been lost or discarded by you;  

 

been damaged as a result of misuse, improper installation, faulty or inadequate maintenance or 
failure to follow instructions furnished by us;  

 

had serial numbers removed, altered, defaced, or rendered illegible; 

 

been subjected to improper or unauthorized repair; or 

 

been damaged due to fire, flood, radiation, or other “acts of God” or other contingencies beyond 

the control of Photometrics. 

8.

 

After the warranty period has expired, you may contact the Photometrics factory or a Photometrics-

authorized representative for repair information and/or extended warranty plans. 

9.

 

Physically damaged units or units that have been modified are not acceptable for repair in or out of 

warranty and will be returned as received.  

10.

 

All warranties implied by state law or non-U.S. laws, including the implied warranties of merchantability 

and fitness for a particular purpose, are expressly limited to the duration of the limited warranties set forth 

above. With the exception of any warranties implied by state law or non-U.S. laws, as hereby limited, the 

forgoing warranty is exclusive and in lieu of all other warranties, guarantees, agreements, and similar 

obligations of manufacturer or seller with respect to the repair or replacement of any parts. In no event 

shall Photometrics’ liability exceed the cost of the repair or replacement of the defective product or part. 

Содержание Cascade 128

Страница 1: ...USER MANUAL Cascade 650 1K 512F 512B 128 and 128...

Страница 2: ...User Manual for Cascade Systems Model 650 Model 1K Model 512F Model 512B Model 128 and Model 128...

Страница 3: ...etrics are registered trademarks of Roper Scientific Inc Mac and Macintosh are trademarks of Apple Computer Inc registered in the U S and other countries Windows and Windows NT are registered trademar...

Страница 4: ...and connectors These items carry no warranty expressed or implied Sealed Chamber Integrity Limited 24 Month Warranty Photometrics warrants the sealed chamber integrity of all our products for a perio...

Страница 5: ...out a return authorization evidenced by an RMA will be sent back freight collect 5 These warranties are effective only if purchased from the Photometrics factory or one of our authorized manufacturer...

Страница 6: ...long an implied warranty lasts when an action may be brought or the exclusion or limitation of incidental or consequential damages so the above provisions may not apply to you 12 When contacting us fo...

Страница 7: ...ws of Member States relating to Electromagnetic Compatibility Product Safety This declaration is based upon compliance of the product to the following standards EN 55022 CISPR 22A RF Emissions Control...

Страница 8: ...r States relating to Electromagnetic Compatibility Product Safety This declaration is based upon compliance of the product to the following standards EN 55022 CISPR 22A RF Emissions Control EN 61000 3...

Страница 9: ...ctromagnetic Compatibility Product Safety This declaration is based upon compliance of the product to the following standards EN 55022 CISPR 22A RF Emissions Control EN 61000 3 series Harmonics and Fl...

Страница 10: ...rd 8 Connecting Your Cascade Camera 9 Adjusting the C Mount Adapter 10 Chapter 3 Operating Features Common Features 11 On Chip Multiplication Gain 11 Offset bias 11 Exposure Readout Modes 11 Triggered...

Страница 11: ...7 Timing Diagram for Overlap Mode when Exposure Time Readout Time14 Figure 8 Trigger First Mode Timing Diagram Overlap Mode 15 Figure 9 Trigger First Mode Timing Diagram Non Overlap Mode 15 Figure 10...

Страница 12: ...tion gain Cascade 1K o Uses a 1004x1002 Front illuminated CCD with 8x8 m pixels o Has one readout amplifier charge multiplication gain Cascade 512F o Uses a 512x512 Front illuminated CCD with 16x16 m...

Страница 13: ...nation of this technology with frame transfer readout makes the Cascade cameras excellent choices for experiments where fast framing and low light sensitivity are required Normal Voltage Clock Standar...

Страница 14: ...discharge ESD To avoid permanently damaging the system please observe the following precautions If you are using high voltage equipment such as an arc lamp with your camera system be sure to turn the...

Страница 15: ...l Measurements on page 34 for more information The Cascade C mount adapter is designed so it can be screwed in or out to change the focal depth Once the focal depth as been changed the adapter is secu...

Страница 16: ...ic com Mail Roper Scientific BV Ir D S Tuijnmanweg 10 4131 PN VIANEN Netherlands FRANCE Tel 33 160 86 03 65 Fax 33 160 86 07 09 E mail princeton instruments wanadoo fr Mail Roper Scientific SARL Z I P...

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Страница 18: ...can safely ship the Cascade system to another location or return it for service if necessary If you have any difficulty with any step of the instructions call Photometrics Customer Service Software Co...

Страница 19: ...e software and the application software for Windows based computers Additional instructions are included for installing a PCI card in your computer and capturing images The Photometrics CD ROM contain...

Страница 20: ...ra 1 Connect either end of the DATA cable to the Cascade PCI card that you have installed in the host computer The DATA cable shown below connects your Cascade camera to the Cascade PCI card This cabl...

Страница 21: ...see below and tighten the screws to lock the adapter in place 2 Spanner Wrench Holes 15 38 cm dia x 15 38 cm deep 1 25 3 18 cm apart 2 Setscrews 050 hex key Figure 2 Adjustable C Mount Adapter Travel...

Страница 22: ...s set to zero 0 Typically the offset bias is subtracted from the sample image for quantitative measurement Since the offset can change based on several factors such as multiplication gain speed etc it...

Страница 23: ...erlap Mode Operation in Non Overlap mode is illustrated in the timing diagram below In this example the exposure time is 10 ms and the readout time is 34 8 ms The total time to take 3 frames is 134 4...

Страница 24: ...settings for overlap mode Note that the clearing mode is set to Pre sequence as opposed to Pre exposure in Non Overlap mode Figure 5 Required Settings for Overlap Mode Operation Note In Overlap mode t...

Страница 25: ...maining at 34 8 ms the time taken to acquire 3 frames will be 184 8 ms 3 50 ms 34 8 ms which is equivalent to a frame rate of 16 2 fps In Overlap mode when exposure time readout time the total time TN...

Страница 26: ...Mode In this mode the camera requires only one trigger to acquire a sequence of frames The camera can operate in overlap or non overlap mode depending on the settings explained in the previous section...

Страница 27: ...riggering mode the camera cannot be operated in Overlap mode AIMO Non AIMO Camera users can choose to run the Cascade 128 in either AIMO or non AIMO mode Each of these software selectable modes of ope...

Страница 28: ...her end The charge multiplication also introduces additional noise quantified by noise factor F Typically F is measured between 1 0 and 1 4 Note As the on chip multiplication introduces additional noi...

Страница 29: ...rsion Gain e ADU Multiplication 10 MHz 1 2 0e ADU 2 1 0e ADU 3 0 5e ADU Table 2 Cascade 1K Readout Speed and Typical Conversion Gains Both the Cascade 650 and the Cascade 1K use a frame transfer CCD w...

Страница 30: ...ximate relationship between the DAC setting and charge multiplication factor for the Cascade 512F 512B 128 and 128 is shown in the graph below Figure 13 DAC Setting vs Charge Multiplication Gain for C...

Страница 31: ...ual for specific information on readout amplifier port selection 1 On Chip Multiplication Amplifier Port 1 Operation of multiplication gain in the Cascade 512F 512B 128 and 128 is similar to that in C...

Страница 32: ...onverted into ADU Analog to Digital units and displayed on the display screen Typical conversion gains for different ports are listed in Tables 2 3 and 4 Your camera is individually calibrated for the...

Страница 33: ...n addition the camera should be operated in Overlap mode This can be achieved by setting the camera to frame transfer mode with clearing mode Pre sequence and number of clears 1 refer to Figure 5 page...

Страница 34: ...adout Speed Multiplication Gain Conversion Gain e ADU Readout Mode Kinetic samples single molecule fluorescence or tracking Multiplication Port 1 10 MHz or 5 MHz Use as required to obtain best signal...

Страница 35: ...24 Cascade User Manual This page intentionally left blank...

Страница 36: ...tions and that the Cascade system s HCK disc is in the host computer s CD drive It is possible that there is a conflict between the new PCI card and a previously installed expansion card With the comp...

Страница 37: ...erformance afforded by the Cascade 128 is highly sensitive to the camera s orientation and environment Optimal cooling can be achieved when the camera is situated in a downward looking position with f...

Страница 38: ...10 92 cm 2 Holes 15 in 38 cm dia x 15 in 38 cm deep 1 25 in 3 18 cm apart C Mount Adapter Thickness Cascade 650 265 in 67 cm Cascade 1K 325 in 83 cm Cascade 650 and 1K Cameras Front View 2 Setscrews...

Страница 39: ...28 Cascade User Manual Cascade 128 Camera Front View...

Страница 40: ...Basic Specifications 29 Camera Dimensions Bottom 7 01 in 17 8 cm 2 90 in 7 37 cm 7 19 in 18 26 cm Cascade 650 Camera Bottom View 7 02 in 17 83 cm 2 90 in 7 37 cm 7 20 in 18 29 cm Cascade 1K Camera Bo...

Страница 41: ...87 in 7 17 in 18 21 cm 09 in 23 cm 1 4 20 UNC 2B thread 30 in 76 cm max deep Cascade 512F 512B and 128 Cameras Bottom View 1 4 20 UNC 2B THREAD 40 in 1 02 cm MAX DEEP 7 38 in 18 75 cm 2 53 in 6 43 cm...

Страница 42: ...Chapter 5 Basic Specifications 31 Focal Plane Measurement 66 in 16 8 mm FOCAL PLANE Cascade 650 Camera Focal Plane 66 in 16 8 mm MECHANICAL 61 in 15 5 mm FOCAL PLANE Cascade 1K Camera Focal Plane...

Страница 43: ...32 Cascade User Manual FOCAL PLANE 63 in 16 0 mm MECHANICAL Cascade 512F and 512B Cameras Focal Plane 63 in 16 0 mm MECHANICAL 58 in 14 7 mm FOCAL PLANE Cascade 128 Camera Focal Plane...

Страница 44: ...Chapter 5 Basic Specifications 33 080 in 2 0 mm Adjustment 65 in 16 5 mm Mechanical 70 in 17 8 mm Optical FOCAL PLANE Cascade 128 Camera Focal Plane...

Страница 45: ...650 Cascade 1K Cascade 512F Cascade 512B Cascade 128 Cascade 128 CCD TI TC253 TI TC285SPD E2V CCD87F E2V CCD97B E2V CCD60 E2V CCD60 Image Type Monochrome Monochrome Monochrome Monochrome Monochrome R...

Страница 46: ...asic Specifications 35 x 1004 Serial Register y 1002 0 0 Cascade 1K CCD Orientation x 512 Serial Register y 512 0 0 Port 1 0 0 Port 2 Cascade 512F 512B CCD Orientation x 128 y 128 0 0 Cascade 128 CCD...

Страница 47: ...lowing connectors are located on the back of the camera DATA connector 20 pin high density connector for data transfer and power POWER connector 5 pin LEMO connector for camera power I O connector DB2...

Страница 48: ...Trigger Invert Input Pin 2 Frame Readout Pin 7 Camera exposing output Pin 8 and Shutter Output Pin 23 is available from Photometrics 1 Trigger Input This input is internally tied high through a 4 7k...

Страница 49: ...t 15 TTL I O data bit 1 TTL level programmable input or output Port 3 16 TTL I O data bit 0 TTL level programmable input or output 17 TTL I O data bit 1 TTL level programmable input or output 18 GND S...

Страница 50: ...Output 12 Vdc Maximum Power Output 96 W Power Supply Weight 2 lb 0 9 kg Note CE certification applies to the Cascade only when the camera system is operated with a CE approved power supply 6 50 in 16...

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Страница 52: ...ion of conformity vii dual readout ports 19 readout speed 21 software compatibility 7 Cascade 650 binning 18 CCD orientation 34 conversion gain 17 declaration of conformity vi multiplication gain 17 r...

Страница 53: ...I card installation 8 Port 1 description 20 Port 2 description 21 POWER connector LEMO 36 pinout 37 Power supply installation 9 specifications 39 Precautions 3 R S Readout modes non overlap 11 overlap...

Страница 54: ...GmbH Rosenheimer Landstr 87 D 85521 Ottobrunn Germany tel 49 89 660 779 3 fax 49 89 660 779 50 email mail roperscientific de JAPAN Nipon Roper K K D 10E 1 3 Nakase Mihama ku Chiba shi Japan 261 8501...

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