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Photometrics Cascade 128 Скачать руководство пользователя страница 34

Chapter 3. Operating Features

23

Summary

Table 5, below, summarizes typical Cascade:512F and 512B settings for two

types of applications and is provided as a setup aid. Actual settings will vary

based on the exact nature of your experiment design.

Application

Readout

Amplifier

(Port)

Readout

Speed

Multiplication

Gain

Conversion

Gain

(e-/ADU)

Readout

Mode

Kinetic
samples
(single
molecule
fluorescence
or tracking)

Multiplication
(Port #1)

10 MHz
or
5 MHz

Use as required
to obtain best
signal-to-noise
ratio (SNR).

Typical DAC
values are around
3000.

#2
(6 e-/ADU)

Overlap
mode
(Frame
Transfer,
Pre-
Sequence)

Fixed samples
(bright field)

Non-
multiplication
(Port #2)

1 MHz or
5 MHz

Not Applicable #1

(4 e-/ADU)

Overlap
or Non-
overlap

Table 6. Application Type and Cascade:512F Setup Parameters

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Содержание Cascade 128

Страница 1: ...USER MANUAL Cascade 650 1K 512F 512B 128 and 128...

Страница 2: ...User Manual for Cascade Systems Model 650 Model 1K Model 512F Model 512B Model 128 and Model 128...

Страница 3: ...etrics are registered trademarks of Roper Scientific Inc Mac and Macintosh are trademarks of Apple Computer Inc registered in the U S and other countries Windows and Windows NT are registered trademar...

Страница 4: ...and connectors These items carry no warranty expressed or implied Sealed Chamber Integrity Limited 24 Month Warranty Photometrics warrants the sealed chamber integrity of all our products for a perio...

Страница 5: ...out a return authorization evidenced by an RMA will be sent back freight collect 5 These warranties are effective only if purchased from the Photometrics factory or one of our authorized manufacturer...

Страница 6: ...long an implied warranty lasts when an action may be brought or the exclusion or limitation of incidental or consequential damages so the above provisions may not apply to you 12 When contacting us fo...

Страница 7: ...ws of Member States relating to Electromagnetic Compatibility Product Safety This declaration is based upon compliance of the product to the following standards EN 55022 CISPR 22A RF Emissions Control...

Страница 8: ...r States relating to Electromagnetic Compatibility Product Safety This declaration is based upon compliance of the product to the following standards EN 55022 CISPR 22A RF Emissions Control EN 61000 3...

Страница 9: ...ctromagnetic Compatibility Product Safety This declaration is based upon compliance of the product to the following standards EN 55022 CISPR 22A RF Emissions Control EN 61000 3 series Harmonics and Fl...

Страница 10: ...rd 8 Connecting Your Cascade Camera 9 Adjusting the C Mount Adapter 10 Chapter 3 Operating Features Common Features 11 On Chip Multiplication Gain 11 Offset bias 11 Exposure Readout Modes 11 Triggered...

Страница 11: ...7 Timing Diagram for Overlap Mode when Exposure Time Readout Time14 Figure 8 Trigger First Mode Timing Diagram Overlap Mode 15 Figure 9 Trigger First Mode Timing Diagram Non Overlap Mode 15 Figure 10...

Страница 12: ...tion gain Cascade 1K o Uses a 1004x1002 Front illuminated CCD with 8x8 m pixels o Has one readout amplifier charge multiplication gain Cascade 512F o Uses a 512x512 Front illuminated CCD with 16x16 m...

Страница 13: ...nation of this technology with frame transfer readout makes the Cascade cameras excellent choices for experiments where fast framing and low light sensitivity are required Normal Voltage Clock Standar...

Страница 14: ...discharge ESD To avoid permanently damaging the system please observe the following precautions If you are using high voltage equipment such as an arc lamp with your camera system be sure to turn the...

Страница 15: ...l Measurements on page 34 for more information The Cascade C mount adapter is designed so it can be screwed in or out to change the focal depth Once the focal depth as been changed the adapter is secu...

Страница 16: ...ic com Mail Roper Scientific BV Ir D S Tuijnmanweg 10 4131 PN VIANEN Netherlands FRANCE Tel 33 160 86 03 65 Fax 33 160 86 07 09 E mail princeton instruments wanadoo fr Mail Roper Scientific SARL Z I P...

Страница 17: ...6 Cascade User Manual This page intentionally left blank...

Страница 18: ...can safely ship the Cascade system to another location or return it for service if necessary If you have any difficulty with any step of the instructions call Photometrics Customer Service Software Co...

Страница 19: ...e software and the application software for Windows based computers Additional instructions are included for installing a PCI card in your computer and capturing images The Photometrics CD ROM contain...

Страница 20: ...ra 1 Connect either end of the DATA cable to the Cascade PCI card that you have installed in the host computer The DATA cable shown below connects your Cascade camera to the Cascade PCI card This cabl...

Страница 21: ...see below and tighten the screws to lock the adapter in place 2 Spanner Wrench Holes 15 38 cm dia x 15 38 cm deep 1 25 3 18 cm apart 2 Setscrews 050 hex key Figure 2 Adjustable C Mount Adapter Travel...

Страница 22: ...s set to zero 0 Typically the offset bias is subtracted from the sample image for quantitative measurement Since the offset can change based on several factors such as multiplication gain speed etc it...

Страница 23: ...erlap Mode Operation in Non Overlap mode is illustrated in the timing diagram below In this example the exposure time is 10 ms and the readout time is 34 8 ms The total time to take 3 frames is 134 4...

Страница 24: ...settings for overlap mode Note that the clearing mode is set to Pre sequence as opposed to Pre exposure in Non Overlap mode Figure 5 Required Settings for Overlap Mode Operation Note In Overlap mode t...

Страница 25: ...maining at 34 8 ms the time taken to acquire 3 frames will be 184 8 ms 3 50 ms 34 8 ms which is equivalent to a frame rate of 16 2 fps In Overlap mode when exposure time readout time the total time TN...

Страница 26: ...Mode In this mode the camera requires only one trigger to acquire a sequence of frames The camera can operate in overlap or non overlap mode depending on the settings explained in the previous section...

Страница 27: ...riggering mode the camera cannot be operated in Overlap mode AIMO Non AIMO Camera users can choose to run the Cascade 128 in either AIMO or non AIMO mode Each of these software selectable modes of ope...

Страница 28: ...her end The charge multiplication also introduces additional noise quantified by noise factor F Typically F is measured between 1 0 and 1 4 Note As the on chip multiplication introduces additional noi...

Страница 29: ...rsion Gain e ADU Multiplication 10 MHz 1 2 0e ADU 2 1 0e ADU 3 0 5e ADU Table 2 Cascade 1K Readout Speed and Typical Conversion Gains Both the Cascade 650 and the Cascade 1K use a frame transfer CCD w...

Страница 30: ...ximate relationship between the DAC setting and charge multiplication factor for the Cascade 512F 512B 128 and 128 is shown in the graph below Figure 13 DAC Setting vs Charge Multiplication Gain for C...

Страница 31: ...ual for specific information on readout amplifier port selection 1 On Chip Multiplication Amplifier Port 1 Operation of multiplication gain in the Cascade 512F 512B 128 and 128 is similar to that in C...

Страница 32: ...onverted into ADU Analog to Digital units and displayed on the display screen Typical conversion gains for different ports are listed in Tables 2 3 and 4 Your camera is individually calibrated for the...

Страница 33: ...n addition the camera should be operated in Overlap mode This can be achieved by setting the camera to frame transfer mode with clearing mode Pre sequence and number of clears 1 refer to Figure 5 page...

Страница 34: ...adout Speed Multiplication Gain Conversion Gain e ADU Readout Mode Kinetic samples single molecule fluorescence or tracking Multiplication Port 1 10 MHz or 5 MHz Use as required to obtain best signal...

Страница 35: ...24 Cascade User Manual This page intentionally left blank...

Страница 36: ...tions and that the Cascade system s HCK disc is in the host computer s CD drive It is possible that there is a conflict between the new PCI card and a previously installed expansion card With the comp...

Страница 37: ...erformance afforded by the Cascade 128 is highly sensitive to the camera s orientation and environment Optimal cooling can be achieved when the camera is situated in a downward looking position with f...

Страница 38: ...10 92 cm 2 Holes 15 in 38 cm dia x 15 in 38 cm deep 1 25 in 3 18 cm apart C Mount Adapter Thickness Cascade 650 265 in 67 cm Cascade 1K 325 in 83 cm Cascade 650 and 1K Cameras Front View 2 Setscrews...

Страница 39: ...28 Cascade User Manual Cascade 128 Camera Front View...

Страница 40: ...Basic Specifications 29 Camera Dimensions Bottom 7 01 in 17 8 cm 2 90 in 7 37 cm 7 19 in 18 26 cm Cascade 650 Camera Bottom View 7 02 in 17 83 cm 2 90 in 7 37 cm 7 20 in 18 29 cm Cascade 1K Camera Bo...

Страница 41: ...87 in 7 17 in 18 21 cm 09 in 23 cm 1 4 20 UNC 2B thread 30 in 76 cm max deep Cascade 512F 512B and 128 Cameras Bottom View 1 4 20 UNC 2B THREAD 40 in 1 02 cm MAX DEEP 7 38 in 18 75 cm 2 53 in 6 43 cm...

Страница 42: ...Chapter 5 Basic Specifications 31 Focal Plane Measurement 66 in 16 8 mm FOCAL PLANE Cascade 650 Camera Focal Plane 66 in 16 8 mm MECHANICAL 61 in 15 5 mm FOCAL PLANE Cascade 1K Camera Focal Plane...

Страница 43: ...32 Cascade User Manual FOCAL PLANE 63 in 16 0 mm MECHANICAL Cascade 512F and 512B Cameras Focal Plane 63 in 16 0 mm MECHANICAL 58 in 14 7 mm FOCAL PLANE Cascade 128 Camera Focal Plane...

Страница 44: ...Chapter 5 Basic Specifications 33 080 in 2 0 mm Adjustment 65 in 16 5 mm Mechanical 70 in 17 8 mm Optical FOCAL PLANE Cascade 128 Camera Focal Plane...

Страница 45: ...650 Cascade 1K Cascade 512F Cascade 512B Cascade 128 Cascade 128 CCD TI TC253 TI TC285SPD E2V CCD87F E2V CCD97B E2V CCD60 E2V CCD60 Image Type Monochrome Monochrome Monochrome Monochrome Monochrome R...

Страница 46: ...asic Specifications 35 x 1004 Serial Register y 1002 0 0 Cascade 1K CCD Orientation x 512 Serial Register y 512 0 0 Port 1 0 0 Port 2 Cascade 512F 512B CCD Orientation x 128 y 128 0 0 Cascade 128 CCD...

Страница 47: ...lowing connectors are located on the back of the camera DATA connector 20 pin high density connector for data transfer and power POWER connector 5 pin LEMO connector for camera power I O connector DB2...

Страница 48: ...Trigger Invert Input Pin 2 Frame Readout Pin 7 Camera exposing output Pin 8 and Shutter Output Pin 23 is available from Photometrics 1 Trigger Input This input is internally tied high through a 4 7k...

Страница 49: ...t 15 TTL I O data bit 1 TTL level programmable input or output Port 3 16 TTL I O data bit 0 TTL level programmable input or output 17 TTL I O data bit 1 TTL level programmable input or output 18 GND S...

Страница 50: ...Output 12 Vdc Maximum Power Output 96 W Power Supply Weight 2 lb 0 9 kg Note CE certification applies to the Cascade only when the camera system is operated with a CE approved power supply 6 50 in 16...

Страница 51: ...40 Cascade User Manual This page intentionally left blank...

Страница 52: ...ion of conformity vii dual readout ports 19 readout speed 21 software compatibility 7 Cascade 650 binning 18 CCD orientation 34 conversion gain 17 declaration of conformity vi multiplication gain 17 r...

Страница 53: ...I card installation 8 Port 1 description 20 Port 2 description 21 POWER connector LEMO 36 pinout 37 Power supply installation 9 specifications 39 Precautions 3 R S Readout modes non overlap 11 overlap...

Страница 54: ...GmbH Rosenheimer Landstr 87 D 85521 Ottobrunn Germany tel 49 89 660 779 3 fax 49 89 660 779 50 email mail roperscientific de JAPAN Nipon Roper K K D 10E 1 3 Nakase Mihama ku Chiba shi Japan 261 8501...

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