Perkin-Elmer RamanStation 400 Series, Руководство по началу работы

Страница: 77 / 98

Using the RamanStation 400 . 77
5. Use the joystick to position the stage.
The joystick operates in the following manner:
– Left / Right joystick movement gives x movement of the stage.
– Top / Bottom joystick movement gives y movement of the stage.
– Twisting the joystick gives z movement of the stage (if purchased):
Left twist (counter clockwise) gives upward stage movement;
Right twist (clockwise) gives downward stage movement.
OR
Use the
Stage Position
controls on the Setup Sample Area XYZ tab to position the
stage.
NOTE: When using the joystick with the snout in the horizontal orientation, although the
movement of the stage will not change, the direction the relative to the on-screen
image will be different.
6. Focus on the sample.
You can use the joystick or Stage Position controls. Alternatively, click
Video
AutoFocus to perform an automatic video focus or
Raman AutoFocus
to focus
using the strongest Raman signal. Refer to the Spectrum on-screen help for more
information.
7. Click .
The RamanStation 400 will start collecting data in the Live display.
8. Adjust the
Laser power (%)
in the Live display to an appropriate value for your sample.
9. Adjust the
Exposure time (s)
.
This will automatically acquire another background.
The sample may now be positioned and aligned correctly for optimized data collection
using the joystick.
10. If required, adjust the Laser power and Exposure time further to optimize the conditions
for your sample.
11. When you have optimized your experimental parameters, click to exit the Live
display.
You can now collect and save spectral data (SOP-4).
NOTE: If you have
Show Live Display
selected on the Setup Instrument Data Collection tab
and you select
Preview
on the Sample Table, then you can preview each sample in
the Live display after pressing
Scan
. When you have adjusted the settings, click
Scan
again to collect data.