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C-7100 Series User Manual                                                                                                                                       PEAK INSTRUMENTS INC. 

 

CONTENT 

 
 

I.

 

Spectrophotometer Overview ...................................................................................................................................1 

 

1.

 

Basic operating principle ...........................................................................................................................................1 

 

2.

 

Performance Indicators Definitions ..........................................................................................................................3 

 

3.

 

Application ................................................................................................................................................................4 

 

4.

 

Using conditions ........................................................................................................................................................4 

 

II.

 

Product features and installation 

 

1.

 

Features ....................................................................................................................................................................5 

 

2.

 

Performance indicators ............................................................................................................................................6 

 

3.

 

Main Functions .........................................................................................................................................................7 

 

4.

 

Structure Introduction ..............................................................................................................................................8 

5.

 

Instrument Installation ……………………………………………………………….………………………………………………………………………10 

 

III.

 

Key definitions and basic operation .........................................................................................................................11 

 

1.

 

Button panel schematic diagram ..............................................................................................................................11 

 

2.

 

Button function description ......................................................................................................................................11 

 

3.

 

Basic operation ..........................................................................................................................................................12 

 

IV. Instrument self-test ..................................................................................................................................................12 

 

1.

 

Communication port inspection……………………………………………………..…………………………………………………………………….12 

 

2.

 

File system check .......................................................................................................................................................12 

 

3.

 

Filter positioning ........................................................................................................................................................12 

 

4.

 

Light positioning ........................................................................................................................................................12 

 

5.

 

Printer Check .............................................................................................................................................................13 

 

6.

 

Tungsten lamp examination ......................................................................................................................................13 

 

7.

 

Deuterium lamp examination ....................................................................................................................................13 

 

8.

 

Signal detector check .................................................................................................................................................13 

 

9.

 

Wavelength Calibration .............................................................................................................................................13 

 

10.

 

Dark current correction ...........................................................................................................................................13 

 

11.

 

System parameters check ........................................................................................................................................13 

 
V. Photometric measurement .........................................................................................................................................14 

 

1.

 

Function description ...................................................................................................................................................14 

 

2.

 

Set the measurement mode .......................................................................................................................................14 

 

3.

 

Set wavelength ...........................................................................................................................................................15 

 

4.

 

Correction 100%T/0Abs ..............................................................................................................................................15 

 

5.

 

Measurement data .....................................................................................................................................................15 

 

6.

 

Delete data .................................................................................................................................................................16 

 

7.

 

Save the file ................................................................................................................................................................16 

8.

 

Open the file …………………………………………………………………………………………………………………………………………………………17 

VI. Quantitative measurement ........................................................................................................................................17 

 

1.

 

Set parameter .............................................................................................................................................................18 

 

2.

 

Standard sample measurement ..................................................................................................................................18 

 

3.

 

Sample measurement ..................................................................................................................................................19 

 

4.

 

Open standard curve ....................................................................................................................................................19 

Содержание C-7100 Series

Страница 1: ...C 7100 Series User Manual PEAK INSTRUMENTS INC C 7100 Series Spectrophotometer Operating Manual and Maintenance Guide PEAK INSTRUMENTS INC Version 1701...

Страница 2: ...12 IV Instrument self test 12 1 Communication port inspection 12 2 File system check 12 3 Filter positioning 12 4 Light positioning 12 5 Printer Check 13 6 Tungsten lamp examination 13 7 Deuterium la...

Страница 3: ...T 0Abs 27 4 Data test 27 X Protein nucleic acid measurement 28 1 Function description 28 2 Set parameter 29 3 Set Zero 30 4 Test 30 XI System setting 30 1 Dark current measurements 31 2 Looking for d...

Страница 4: ...ght is proportional to the product of the solution concentration and thickness Its digital expression A KCL LogI I0 LogT Premise absorption laws established numeric expression the incident light is mo...

Страница 5: ...he absorbance When quantitative analysis in order to obtain sufficient measuring signal the slit should be larger in the qualitative analysis of the use of a smaller slit when the entrance slit and th...

Страница 6: ...from another perspective to understand this concept will more user friendly first the monochromator exit slit represents not just the physical size or geometry it also represents the optical sense th...

Страница 7: ...ernal electronic noise inherent in the instrument such as power supply amplifier AD conversion and the like To reduce noise improve signal to noise ratio there must be a good electrical design Noise m...

Страница 8: ...and 100 T and other control functions as well as the concentration of computing and data processing functions of a variety of methods but also to prevent user errors with special features no worries...

Страница 9: ...T 0 002A 0 0 5A 0 004A 0 5 1A Photometric Repeatability 0 15 T 0 100 T 0 001A 0 0 5A 0 002A 0 5 1A Stray Light 0 03 T 220nm 360nm Stability 0 001A h 500nm Photometric Range 0 200 T 0 3 3 0A 0 9999C 0...

Страница 10: ...C 7100 Series User Manual PEAK INSTRUMENTS INC 7 3 Main Functions...

Страница 11: ...C 7100 Series User Manual PEAK INSTRUMENTS INC 8 4 Structure introduction Overall structure consists of three parts optical system power system and micro computer system 1 Top view 2 Bottom view...

Страница 12: ...eration maximum energy sources in the vicinity of 230nm 486 0nm and 656 1nm and has two characteristic lines can be used for instrument calibration wavelength accuracy in the visible region Stability...

Страница 13: ...nt to meet the foregoing requirements the ambient temperature 10 35 relative humidity less than 85 operating voltage 220 3 22 V 50 3 1 H3 3 The instrument is placed on a horizontal platform the instru...

Страница 14: ...tput button SAVE File storage button LOAD File open button ESC Back cancel button test stop button CLEAR Clear key to delete the input data delete files GO Set the wavelength ZERO Adjust 100 T and 0Ab...

Страница 15: ...t 1 Communication port inspection Check the communication port of the instrument is working properly The result is displayed correct as and an error as the buzzer alarm 2 File system check Check the i...

Страница 16: ...nt is working properly Correct then the pop up boxes please confirm whether the user input wavelength calibration if no user input within five seconds then skip this If the argument is wrong wavelengt...

Страница 17: ...ry work environment which is the instrument re calibration dark current set the working parameters etc and then enter the main menu V Photometric measurement 1 Function description Photometric measure...

Страница 18: ...the set range buzzer alarm 4 Correction 100 T 0Abs In two samples simultaneously into the two slots reference solution and then press the ZERO key the instrument will be blank correction in the curren...

Страница 19: ...operation will delete all the test data currently under test Select Yes the file will be deleted all the data select No will return the test window 7 Save the file Press the Save button to save the cu...

Страница 20: ...dow has all the photometric data file list Select the appropriate data file and press ENTER the file will be read into all the test data and then automatically enters the measurement mode VI Quantitat...

Страница 21: ...hrough zero and second order fitting 2 Curve building method there are standard sample method and coefficient method Stand sample method is to prepare sample first build curve and then test sample Coe...

Страница 22: ...lculated automatically according to the parameters of the curve displayed on the screen If the parameter is wrong then the buzzer alarm exit to establish the curve function 3 Sample test Put sample in...

Страница 23: ...ys the upper and lower limits 1 Set test time Test time is the total time of the entire test 2 Set the measurement mode The measurement mode is absorbance transmission or energy Choose different measu...

Страница 24: ...eries User Manual PEAK INSTRUMENTS INC 21 3 Data test Press START to start measuring the current real time map will be on the screen 4 Data list Press Menu key to check the scanning data after scannin...

Страница 25: ...VIII Wavelength scanning 1 Function description In the set wavelength range in a certain wavelength interval to record the absorbance of the sample and the energy transmittance value the results are p...

Страница 26: ...ed depends on the quantity of data collecting on single point wavelength the faster the speed the less the collecting quantity 6 select to make data smoothing The function for data smoothing is to red...

Страница 27: ...eries User Manual PEAK INSTRUMENTS INC 24 4 Create a user baseline corrected blank Before starting the measurement the user must establish a baseline that is correction of the reference sample 100 T a...

Страница 28: ...UMENTS INC 25 5 Begin testing The tested samples were placed in the light path press START to start the test 6 Peaks and troughs After the test is finished you can choose to find peaks and valleys pea...

Страница 29: ...est 1 Function description Multi wavelength for the user is needed to test a sample while measuring the transmittance or absorbance at a wavelength setting of several functions the user interface can...

Страница 30: ...hen lose the next one until all shall be measured wavelengths losers 3 Correction 100 T 0Abs After these parameters are set place two reference solution for both colorimetric anti tank and then press...

Страница 31: ...asurement 1 Function description Protein nucleic acid comprising two tests Measurement of protein and single stranded nucleic acid testing The curve and parameter is installed inside instrument for th...

Страница 32: ...C 7100 Series User Manual PEAK INSTRUMENTS INC 29 2 Parameter selection...

Страница 33: ...Manual PEAK INSTRUMENTS INC 30 3 Set zero Put reference solution into light path after selecting mode press ZERO key to set zero 4 Test Put sample solution into light path press Start key to measure...

Страница 34: ...ction for full range of dark current Please make sure to cover the sample chamber lid when test dark current 2 Looking for deuterium lamp curve This function is to locate 656 1nm wavelength characteri...

Страница 35: ...entry press Esc to abandon input Note The time and date after the instrument is switched off will not power down lost 4 Light source management Of deuterium lamps and tungsten light source light switc...

Страница 36: ...C 7100 Series User Manual PEAK INSTRUMENTS INC 33 5 General 6 File system...

Страница 37: ...User Manual PEAK INSTRUMENTS INC 34 7 USB storage device 8 Restore factory settings This operation will restore all system configuration information this operation does not affect the system baseline...

Страница 38: ...lid to prevent the impact of volatile gases to light thus affecting the accuracy of the test equipment 3 Any optical instrument road section screws and nuts are not going to loose to prevent the opti...

Страница 39: ...determine the initial failure is part of the nature of the general failure of the instrument s internal and external points life failures and occasional failures of the points and the user can handle...

Страница 40: ...l dealer Motor driver chip on the motherboard Please contact the manufacturer or your bad TD62083 local dealer Motherboard connector cable loose signal Opening the housing service personnel board unde...

Страница 41: ...se be careful burns 3 Find the objects shown in the following figure loose fastening sheet metal screws pull out a tungsten lamp and the new tungsten lamp according to the original position seated and...

Страница 42: ...note deuterium lamp light mouth will toward objective and plug it in the power supply board Unscrew the two screws and the cable to remove deuterium lamp 4 Turn on the instrument make deuterium lamp...

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