Components
Components
Install Spectr
Install Spectrometr
ometry Softwar
y Softwaree
The latest version of Spectrometry software is required to use the
Spectrometer. The UV-VIS Spectrometer can be used with a Windows
or Mac computer. Download the software at pasco.com/downloads
Connect t
Connect to Spectr
o Spectrometr
ometry Softwar
y Softwaree
1. Connect the AC adapter to the power jack on the spectrometer.
Connect the other end to an electrical outlet.
2. Connect the USB cable to the USB port on the spectrometer.
Connect the other end to a USB port on a computer.
3. Set the power switch to the on position to turn on the
Spectrometer.
4. Open Spectrometry on your computer.
Important: You
may receive a notification to install a firmware
update. Click Yes to update the firmware if prompted.
W
Warm-Up Time
arm-Up Time
Before collecting data, turn on the UV-VIS Spectrometer and allow it
to warm up for 10 minutes. Measurements may fluctuate if the
spectrometer is not provided with enough time to warm up.
Pr
Prepar
epare Solutions for Analysis
e Solutions for Analysis
Solutions to be analyzed with the spectrometer need to be placed in
cuvettes.
CAUTION: The semi-micro cuvettes are made from a proprietary
polycyclical olefin that can be used with most polar solvents, acids,
and alkaline solutions. Check your specific solvent for interactions
before allowing samples to sit for any period of time in the
spectrometer to avoid damaging the equipment.
Note: It is recommended to use quartz cuvettes (sold
separately) when analyzing wavelengths below 220 nm.
• Fill the cuvette ¾ full with the sample solution and cap the cuvette.
• Clean the smooth sides of the cuvette using a lint free wipe before
inserting it into the spectrometer.
• When inserting a cuvette, make sure that the smooth side faces
the light source.
You will also need to prepare a reference solution for calibration
when preparing solutions for analysis. The reference solution should
be the solvent used to make the sample solutions.
Analyz
Analyze the Absorbance of a Solution
e the Absorbance of a Solution
Measure the wavelengths of light a solution absorbs and transmits.
1. Prepare the samples for analysis.
a. Fill the cuvettes ¾ full with the sample solutions and cap.
b. Prepare a reference solution by filling one cuvette ¾ full with
the solvent used to make the sample solutions.
2. Connect the Spectrometer to Spectrometry.
3. Calibrate the spectrometer.
a. Select the Analyze Solution page
.
b. Cover the spectrometer to block ambient light and click
Calibrate Dark
.
c. Insert the reference solution in the spectrometer then click
Calibrate Reference
.
4. Collect data.
a. Insert a sample in the spectrometer then click Record
.
b. Use the Tools to adjust the smoothing and number of scans
to average as needed.
c. Click Stop
when you are satisfied with the results.
5. Analyze the data.
a. Click
to use the coordinate tool locate the peak(s) for each
sample.
b. Use the other graph tools as needed to perform further
analysis.
Power Switch
Power Switch
Use to turn the Spectrometer on and off.
1
Power Jack
Power Jack
Connect to an electrical outlet using the AC Adapter.
CAUTION: Do not disconnect from power until the fan stops
running to avoid damage to the equipment.
2
Light Source Location
Light Source Location
When inserting a cuvette that has only two smooth sides, make
sure one of the smooth sides faces the light source.
3
Test Chamber
Test Chamber
Insert a cuvette containing a solution or the Fiber Optics Cable.
4
USB Port
USB Port
Connect to a computer USB port using the USB cable.
5
UV-VIS Spectrometer | SE-3607
Product Guide
2
012-16942A