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4.
In the
Magnification
field enter the current magnification
•
Some magnification values (default ones 4x, 10x, 20x, 40x and 100x) are preset in the drop-down list.
•
If user must insert a different magnification value, double click in the
Magnification
field. The following dialog box ap
-
pears:
•
From here user can enter any desired magnification value.
5.
Focus the ruler under the microscope.
•
Along with the previous dialog box a red line appears on the image. This line is the calibration line.
6.
Align the two ends of the red line with the ruler scale (try to use as many notches as possible to keep the calibration
accuracy).
7.
Set the desired unit (micrometer, millimeter or nanometer) in the
Calibration
dialog box.
8. Insert the length of the drawn line in the
Actual Length
field (using the same procedure described above).
•
Micrometric slide has two rulers (see chapter 11). One is 1 mm/100 (for biological microscopes) and the other is
10mm/100 (for stereomicroscopes).
•
When using the ruler 1mm/100 the interval between two long lines is 100
m
m, while using the ruler 10mm/100 the inter
-
val between two long lines is 1000
m
m.
9.
If everything is fine, click
OK
to end the calibration. The new magnification (the number you entered in the Magnification
edit box) will be available in the Magnification drop-down list.
10.
Repeat the same procedure for each magnification available in your microscope.
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