13
5. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
6. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Particles
CNR.
7. Remove the plate containing the Mag-Bind® Particles CNR from the magnetic
separation device.
8. Add 600 µL RXT Wash Buffer to each well. Pipet up and down 20 times or shake for 2
minutes to mix thoroughly.
Note:
RXT Buffer must be diluted with ethanol before use. Please see Page 6 for
instructions.
9. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
10. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Particles
CNR.
11. Remove the plate containing the Mag-Bind® Particles CNR from the magnetic
separation device.
12. Repeat Steps 8-11 for a second RXT Wash Buffer wash step.
13. Add 600 μL RNA Wash Buffer II to each well. Pipet up and down 10 times or shake for
2 minutes to mix thoroughly.
Note:
RNA Wash Buffer II must be diluted with ethanol before use. Please see Page 6
for instructions.
14. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
M2839 Mag-Bind® Blood RNA 96 Protocol
