www.microscopenet.com
18
(a)
(b)
(c)
(d)
Note:
When raising the mechanical stage, do not make
contact with the objective.
3) Turn the desired objective into light path.
4) Turn the annular ring disk to put the corresponding
ring into light path, i.e. if you are using the 4
0X
phase contrast objective, you should turn the disk
at
40
as shown in
Fig. 16
.
5) Centering the annular ring.
a. Remove one eyepiece from the microscope eyepiece tube and
insert the centering telescope as shown in
Fig. 17
.
b. Observe from the telescope. The bright ring and dark ring
should be coincided with each other as shown in
Fig.18 (d)
.
c. If the ring images are not clear, turn the top of telescope
until both ring images are in focus.
d. If the bright ring is still obscure as in
Fig.18 (b)
, adjust
the condenser focus knob.
e. If the two ring images are not coincided as shown in
Fig.18 (c)
:
Turn the condenser centering screws
(Fig. 19)
to
move the dark spot to the center until two ring
images are coincided, or
Turn the adjustment knobs
(Fig. 19)
to move the
dark spot to the center until two ring images are
coincided.
f. Remove the centering telescope and replace it with
the eyepiece.
g. Put the specimen on the stage and adjust the
illumination, focusing, etc following the instructions in
this manual.
Note:
The phase contrast condenser will be working as a
conventional Abbe condenser if the annular ring
disk being put at
BF
position.
The phase contrast condenser will be working as a
dry darkfield condenser if the annular ring disk
being put at
DF
position
.
6) Performing the phase contrast observation.
After centering the condenser ring plate, perform
the phase contrast observation the same way as a normal
bright field microscope.
Note:
When change to another phase contrast objective and corresponding condenser
ring plate, the focusing and centering of bright ring and dark ring should be repeated
following the procedures from
5)-b
to
5)-f
.
Fig.17
Fig.16
40
Fig.18
Fig.19
Adjustment Knobs
Condenser Centering Screws
