Olympus IX81-ZDC2 Скачать руководство пользователя страница 8

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6. Restrictions on combined modules

 · The module that can be mounted on the left side port is only a TV adapter that can project the primary image in 1X 

magnification, that is, the U-TV1X, U-DPCAD or U-SIP. (Optimal AF performance cannot be achieved with other combination.)

 · Note on stage
  The IX-SVL2 stage cannot be combined with this microscope.
 · Notes on observation tube

a) When the U-TBI90 observation tube is used, the image in the peripheral part of eyepiece’s visual field may be 

obscured (about 5%). In addition, no intermediate attachment can be used with the U-TBI90.

b) When the U-BI90/BI90CT observation tube is used together with a 4X or 10X objective, the image in the peripheral 

part of eyepiece’s visual field may be obscured. In addition, the IX2-CA2X magnification changer lens unit cannot be 
used with the U-BI90/BI90CT (it becomes usable by mounting the U-EPA2 eye-point adjuster (occupying only 1 
stage) on the U-BI90/U-BI90CT).

 · Notes on intermediate attachments

a) Only one U-EPA2 eye-point adjuster can be mounted (it cannot be used together with other intermediate attach-

ments).

b) Only one U-FWO filter wheel can be mounted (it cannot be used together with other intermediate attachments).
c) When the IX-ATU intermediate attachment and GX-SPU side port unit are combined, the image in the peripheral part 

of eyepiece’s visual field may be obscured if the magnification changer knob of the microscope frame is set to 1X.

d) The U-CA/U-ECA/U-ECA1.6X magnification changer cannot be used in combination with the microscope frame.

 · When the IX-ATU intermediate attachment and U-TR30H trinocular observation tube are combined, the only TV adapter 

that can be used is the U-TV1X.

 · When the GX-SPU side port unit and a TV camera are used, the only TV adapter that can be used is the U-TV1X.
 · The FN of the right side port is specified as 16. When a 10X or lower-power objective a CCD camera are used, the 

brightness in the peripheral area may be slightly insufficient.

 · When a 2X, 4X or 10X objective is used with FN 22, the brightness in the peripheral area may be slightly insufficient. 

(This problem does not occur with FN 16.)

 · When a 10X objective is used in transmitted light observation using the IX2-ULWCD condenser, the illumination NA 

may become insufficient and the resolution may degrade. Also, optimum phase contrast observation is not available 
using a phase contrast objective (PH1 or PH2).

 · When using the IX2-RFAW, remove the light shield @ in the excitation 

filter slider on the left illuminator to prevent it from coming in the way of 
operation.

  Some filter sliders are not usable with this microscope.

 · Optimum phase contrast observation is not available when the IX2-ILL30 transmitted light illumination column and a 

phase contrast observation (PH1 or PH2) are used.

 · AF does not function normally when the U-LH100 transmitted light IR lamp housing is used. To achieve AF, it is required 

to close the shutter or turn off the light during AF.

 · The IBMU/IBML temperature retention box cannot be used because a clearance is produced between the tempera- 

ture retention box and transmitted light illumination column.

 · When performing autofocusing in the continuous focus mode, use the provided IR slider to prevent the IR laser from 

leaking  into  the  observation  light.  Note  that,  when  this  slider  is  used,  fluorescent  dyes  with  IR-domain  absorption 
wavelengths (700 nm or more) can become unusable or their brightness is reduced.

  When this slider is used, the fluorescence mirror unit used in the DIC observation should be the U-MDICT.
 · If the I2-ZDC2PCB control board for exclusive use with IX81-ZDC2 is installed on the IX2-UCB control box, uninstall 

other focus control boards.

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Содержание IX81-ZDC2

Страница 1: ...oscope Model IX81 ZDC2 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the work desk for future reference A X 7 9 0 6 ...

Страница 2: ... likely to cause harmful interference in which case the user will be required to correct the interference at his own expense FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Compliance This device complies with the requirements of both directive 2004 108 EC concerning electromagnetic compati...

Страница 3: ...6 7 8 11 12 IMPORTANT Be sure to read this section for safe use of the equipment 1 MODULE NOMENCLATURE 2 NOMENCLATURE 3 OPERATION 4 SPECIFICATIONS 5 TROUBLESHOOTING GUIDE 1 5 CONTENTS IX81 ZDC2 ...

Страница 4: ... equipment damage 7 To prevent the microscope system from falling down do not install modules which cause the microscope system height to exceed 1 meter 8 Do not use an objective other than those specified to be applicable see page 4 Safety Symbols The following symbols are found on the microscope Study the meaning of the symbols and always use the equipment in the safest possible manner Symbol Ex...

Страница 5: ...for example electrical equipment that is being switched on or off which could cause ignition of a fire Also remember to always use it only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean them use a lint free soft cloth slightly moistened with a diluted neutral detergent 3 Do not disassemble or modify any...

Страница 6: ...multi point time lapse observation 2 Continuous focus mode In this mode the laser beam is focused continuously on the top surface of the cover glass of the glass bottom dish at the same time as the target cell During this the focus is corrected automatically even when the change in environmental temperature due to heat cause defocusing The range in which continuous focusing is possible is determin...

Страница 7: ... 60X O 1 42 0 15 UPLSAPO 60X O UPLSAPO 100X O 1 35 1 40 0 15 0 13 UPLFLN 100X O2 UPLFLN 100X OI2 1 30 1 30 0 60 0 20 0 20 UPLSAPO 20X UPLSAPO 40X2 UPLSAPO 60X W 0 75 0 95 1 20 0 60 0 18 0 28 UPLFLN 20X UPLFLN 40X UPLFLN 60X 0 50 0 75 0 90 2 10 0 51 0 20 LUCPLFLN 20X LUCPLFLN 40X LUCPLFLN 60X 0 45 0 60 0 70 6 60 7 80 2 70 4 00 1 50 2 20 UAPO 40X OI3 340 1 35 0 65 0 1 Compatible with one shot focus ...

Страница 8: ...sed the only TV adapter that can be used is the U TV1X The FN of the right side port is specified as 16 When a 10X or lower power objective a CCD camera are used the brightness in the peripheral area may be slightly insufficient When a 2X 4X or 10X objective is used with FN 22 the brightness in the peripheral area may be slightly insufficient This problem does not occur with FN 16 When a 10X objec...

Страница 9: ...r with IX ADUCD Eyepieces WHN10X WHN10X H Stage IX2 SFR IX2 GS IX2 SP Can be used together with IX MVR Observation Tube U BI90CT U BI90 U TBI90 U TR30H 2 Used together with IX ATU Transmitted Illumination Column Fluorescence Mirror Unit Cassette IX2 RFACA IX2 RFAC Reflected Fluorescence Illuminator IX2 RFA IX2 RFAL IX2 RFAW IX2 RFAEVA 2 IX2 ARCEVA IR slider Microscope Frame IX81F ZDC2 IX81S1F ZDC2...

Страница 10: ...em Focus Handle Interface U IFFH CCD camera 2 Focus Handle U FH AF port switching lever Pulled out position OUT Pushed in position IN Wavelength 785 nm Beam divergence Objectives attached 0 to 1 49 NA Depend on objective lens 2Objectives removed 0 14 to 0 03 deg Depend on offset lens position Pulse duration 2 5 ms Pulse repetition rate 100 Hz Maximum power output 127 5 µW AF port switching unit PC...

Страница 11: ...be obtained by judging the image resolution and contrast When a satisfactory image is not obtained after focusing rotate the correction collar to the left and right refocus each time and compare the images at both sides Then rotate the collar in the direction yielding a better image and rotate the collection collar to the left and right refocus each time and compare the images Repeat this cycle un...

Страница 12: ...ded Make sure the oil is free of air bubbles a To check for air bubbles remove the eyepieces completely open the field iris diaphragm and aperture iris diaphragm and look at the objective exit pupil looking like a bright circle in the observation tube Any air bubbles can be seen in this way b To remove air bubbles slightly rock the revolving nosepiece manually to engage and disengage the oil immer...

Страница 13: ...t mode Offset Setup of the amount of compensation between the cover glass top surface and target position AF zone AF search zone AF center width and range In continuous focus mode Confirm that the cover glass top surface is detected in the one shot mode before switching to the continuous focus mode For details refer to the instruction manual and Help of the application When using the MetaMorph app...

Страница 14: ...servations Field number 22 AF focusing speed One shot focus mode only Approximately 1 to 5 sec from the focusing range Excluding the offset drive time Note The AF speed may slow down depending on the PC setup AF repeatability 20X and 40X objectives 0 1 μm with constant temperature 0 17 μm with less than 5 C temperature change 60X objective or 40X 60X 100X oil water immersion objectives 0 07 μm wit...

Страница 15: ...sh is focused Set the AF zone so that its center is located on the interface between the culture fluid and glass bottom dish and the AF zone width is 10 µm 10 b Autofocusing takes long time or fails AF is set improperly Set up AF properly For the setting method refer to the instruction manual for the application software 10 Electrical noise is interfering with op eration Ground the IX2 UCB Be also...

Страница 16: ...2 Printed in Japan on May 19 2010 M 003 01 ...

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