Olympus IX81-ZDC2 Скачать руководство пользователя страница 5

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IX81-ZDC2

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Left side panel

  Control or adjustments using procedures other than those described in this manual may result in hazardous 

exposure to the laser radiation.

  Warning labels

  Warning labels related to laser beam are attached to the microscope. Always heed the warnings.

Warning label position (AF sensor unit)

  When a warning label becomes dirty or is peeled off, contact Olympus for replacement.

 

1

  Getting Ready

1. The microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.
  (For the operating conditions, refer to chapter 4, “SPECIFICATIONS” on page 11.)
3. For the objectives applicable to the microscope, see the list on page 4.

 

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  Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe 

gently using a piece of cleaning paper (or clean gauze).

  If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute 

alcohol.

  Since the absolute alcohol is highly flammable, it must be handled carefully.
  Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical 

equipment that is being switched on or off, which could cause ignition of a fire. 

  Also remember to always use it only in a well-ventilated room.

2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean 

them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble or modify any part of the unit as this could result in malfunction or reduced performance.
  Particularly, the stage support cannot be removed because it has been bonded after adjustment.
4. When not using the microscope system, make sure that the lamp housing is cool enough, and cover the microscope 

system with the dust cover before storage.

5. Before disposing of this microscope, be sure to follow the regulations and rules of your local government.

 

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  Caution

  If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, 

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

CAUTION

The following symbols are used to set off text in this instruction manual.

 

  : Indicates that failure to follow the instructions in the warning could result in bodily 

harm to the user and/or damage to equipment (including objects in the vicinity of the 
equipment).

 

    Indicates that failure to follow the instructions could result in damage to equipment.

  } 

: Indicates commentary (for ease of operation and maintenance).

CAUTION

CAUTION

Содержание IX81-ZDC2

Страница 1: ...oscope Model IX81 ZDC2 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the work desk for future reference A X 7 9 0 6 ...

Страница 2: ... likely to cause harmful interference in which case the user will be required to correct the interference at his own expense FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Compliance This device complies with the requirements of both directive 2004 108 EC concerning electromagnetic compati...

Страница 3: ...6 7 8 11 12 IMPORTANT Be sure to read this section for safe use of the equipment 1 MODULE NOMENCLATURE 2 NOMENCLATURE 3 OPERATION 4 SPECIFICATIONS 5 TROUBLESHOOTING GUIDE 1 5 CONTENTS IX81 ZDC2 ...

Страница 4: ... equipment damage 7 To prevent the microscope system from falling down do not install modules which cause the microscope system height to exceed 1 meter 8 Do not use an objective other than those specified to be applicable see page 4 Safety Symbols The following symbols are found on the microscope Study the meaning of the symbols and always use the equipment in the safest possible manner Symbol Ex...

Страница 5: ...for example electrical equipment that is being switched on or off which could cause ignition of a fire Also remember to always use it only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean them use a lint free soft cloth slightly moistened with a diluted neutral detergent 3 Do not disassemble or modify any...

Страница 6: ...multi point time lapse observation 2 Continuous focus mode In this mode the laser beam is focused continuously on the top surface of the cover glass of the glass bottom dish at the same time as the target cell During this the focus is corrected automatically even when the change in environmental temperature due to heat cause defocusing The range in which continuous focusing is possible is determin...

Страница 7: ... 60X O 1 42 0 15 UPLSAPO 60X O UPLSAPO 100X O 1 35 1 40 0 15 0 13 UPLFLN 100X O2 UPLFLN 100X OI2 1 30 1 30 0 60 0 20 0 20 UPLSAPO 20X UPLSAPO 40X2 UPLSAPO 60X W 0 75 0 95 1 20 0 60 0 18 0 28 UPLFLN 20X UPLFLN 40X UPLFLN 60X 0 50 0 75 0 90 2 10 0 51 0 20 LUCPLFLN 20X LUCPLFLN 40X LUCPLFLN 60X 0 45 0 60 0 70 6 60 7 80 2 70 4 00 1 50 2 20 UAPO 40X OI3 340 1 35 0 65 0 1 Compatible with one shot focus ...

Страница 8: ...sed the only TV adapter that can be used is the U TV1X The FN of the right side port is specified as 16 When a 10X or lower power objective a CCD camera are used the brightness in the peripheral area may be slightly insufficient When a 2X 4X or 10X objective is used with FN 22 the brightness in the peripheral area may be slightly insufficient This problem does not occur with FN 16 When a 10X objec...

Страница 9: ...r with IX ADUCD Eyepieces WHN10X WHN10X H Stage IX2 SFR IX2 GS IX2 SP Can be used together with IX MVR Observation Tube U BI90CT U BI90 U TBI90 U TR30H 2 Used together with IX ATU Transmitted Illumination Column Fluorescence Mirror Unit Cassette IX2 RFACA IX2 RFAC Reflected Fluorescence Illuminator IX2 RFA IX2 RFAL IX2 RFAW IX2 RFAEVA 2 IX2 ARCEVA IR slider Microscope Frame IX81F ZDC2 IX81S1F ZDC2...

Страница 10: ...em Focus Handle Interface U IFFH CCD camera 2 Focus Handle U FH AF port switching lever Pulled out position OUT Pushed in position IN Wavelength 785 nm Beam divergence Objectives attached 0 to 1 49 NA Depend on objective lens 2Objectives removed 0 14 to 0 03 deg Depend on offset lens position Pulse duration 2 5 ms Pulse repetition rate 100 Hz Maximum power output 127 5 µW AF port switching unit PC...

Страница 11: ...be obtained by judging the image resolution and contrast When a satisfactory image is not obtained after focusing rotate the correction collar to the left and right refocus each time and compare the images at both sides Then rotate the collar in the direction yielding a better image and rotate the collection collar to the left and right refocus each time and compare the images Repeat this cycle un...

Страница 12: ...ded Make sure the oil is free of air bubbles a To check for air bubbles remove the eyepieces completely open the field iris diaphragm and aperture iris diaphragm and look at the objective exit pupil looking like a bright circle in the observation tube Any air bubbles can be seen in this way b To remove air bubbles slightly rock the revolving nosepiece manually to engage and disengage the oil immer...

Страница 13: ...t mode Offset Setup of the amount of compensation between the cover glass top surface and target position AF zone AF search zone AF center width and range In continuous focus mode Confirm that the cover glass top surface is detected in the one shot mode before switching to the continuous focus mode For details refer to the instruction manual and Help of the application When using the MetaMorph app...

Страница 14: ...servations Field number 22 AF focusing speed One shot focus mode only Approximately 1 to 5 sec from the focusing range Excluding the offset drive time Note The AF speed may slow down depending on the PC setup AF repeatability 20X and 40X objectives 0 1 μm with constant temperature 0 17 μm with less than 5 C temperature change 60X objective or 40X 60X 100X oil water immersion objectives 0 07 μm wit...

Страница 15: ...sh is focused Set the AF zone so that its center is located on the interface between the culture fluid and glass bottom dish and the AF zone width is 10 µm 10 b Autofocusing takes long time or fails AF is set improperly Set up AF properly For the setting method refer to the instruction manual for the application software 10 Electrical noise is interfering with op eration Ground the IX2 UCB Be also...

Страница 16: ...2 Printed in Japan on May 19 2010 M 003 01 ...

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