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Useful Information
The size of the specimen image for observation is obtained by multiplying the eyepiece magnification by the objective
magnification. This value is referred to as total magnification.
Example: Eyepiece (10X) x Objective (40X) = 400X
} The image observed through the microscope moves in directions
opposite to the actual up-down and left-right movements of the
specimen.
Resolution represents how finely a lens is able to distinguish the details of a specimen.
Resolution is mainly determined by the ability of the objective and scarcely related to that of the eyepieces. The function
of the eyepieces is to just magnify an image already resolved by the objective.
In case total magnifications are the same, better resolution is obtained with the combinations which have higher
magnification of objective.
The field number is the diameter of the image observed through an eyepiece, indicated in millimeters.
Eyepiece 10X : 20 mm WHSZ15X-H:16 mm
The actual field of view is the size of the specimen actually
observed in the microscope. This makes it possible to find out
approximate size of the specimen.
Actual field of view = Field number/Objective magnification
When the eyepiece field number 20 and the objective
magnification 10X are used, the actual field of view is equal to:
Actual field of view = 20/10 = 2.0 mm
How to track a microscopic image
Total magnification
Actual field of view
Field number (FN)
Resolution
Direction of moving the image
To track an image
moving leftwards
(or to move the
specimen image
rightwards):
Move the slide
glass to the
left.
Move the slide
glass down.
To move the
specimen image
upward:
Approx. 1.0 mm,
about half of actual
field of view.
Actual field of
view: 2.0 mm
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