21
CKX53
B
A
· The contrast of the image becomes higher.
· The discoloring of the specimen outside of the field of view being
observed.
Rotate the field iris diaphragm lever a in the arrow direction gradually
and confirm the field iris diaphragm position. (A in the picture)
Rotate the field iris diaphragm centering knobs individually and adjust
so that the image of the field iris diaphragm comes to the center of
the field. (B in the picture)
Rotate the field iris diaphragm lever a in the direction opposite to
the arrow direction and open the field iris diaphragm until its image
inscribes the field of view. If the eccentricity is found after this, try
centering again. (C in the picture)
Then, rotate the field iris diaphragm lever a in the direction opposite
to the arrow direction furthermore, and open the field iris diaphragm
until its image circumscribes the field of view. (D in the picture)
4
5
6
7
Effects of field iris diaphragm
D
C
4-6 Observing with the objective with correction collar (option)
If the thickness of the cover glass is not matched with the thickness
scale of the objective with correction collar, the objective cannot exhibit
its performance. When using the objective with correction collar, adjust
the correction collar.
Objective with correction collar
LUCPLFLN20XPH
LUCPLFLN40XPH
Adjustment method
If the thickness of the cover glass is known, rotate the correction collar
a to match the scale with the thickness of the cover glass.
If the thickness of the cover glass is not known, operate the correction
collar a and the fine focusing knob alternately to search the best
contrast position.
While rotating the revolving nosepiece, do not touch the
correction collar
a
.
NOTE
Hold and slide the selection lever of the fluorescence mirror unit slider
and engage the B-excitation fluorescence mirror unit in the light path.
Hold and slide the knob of the filter slider for reflected light illumination
and engage the empty hole of the slider in the light path.
Engage the 10X objective in the light path, place the specimen on the
stage and bring the specimen into focus approximately.
If the specimen is dark, the center of the mercury burner
may be displaced. In this case, perform the centering of the
mercury burner first. (See page 43.)
1
2
3
TIP
4-5 Adjusting the field iris diaphragm in fluorescence observation (Fluorescence set)
a
1
2
5
a
