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CKX53

2-2 Fluorescence set

The numbers assigned to operating portions indicate operating portions described in "9-2 Summary of
fluorescence observation procedures" (page 48).

TIP

Main switch

Revolving nosepiece

Coarse focusing knob

…

Collector lens focusing knob

Umbra shield

(ambient light shieldingplate)

Field iris diaphragm
centering knob

Field iris diaphragm lever

Filter slider for reflected light illumination

Burner

centering

knobs

Mirror focusing screw

(Back of lamp housing)

Camera Adapter

fixing knob

Fine focusing knob

†

Main switch

(back of light source)

X-axis knob

Y-axis knob

Light path selection

lever

For details, see the instruction
manual provided with U-RFL-T.

TIP

For details, see the instruction
manual provided with U-HGLGPS.

TIP

Fluorescence mirror

unit selection lever

Содержание CKX53

Страница 1: ...pe we recommend that you study this manual thoroughly before operating the microscope and always keep this manual at hand when operating the microscope Retain this instruction manual in an easily acce...

Страница 2: ...designed to provide reasonable protection against harmful interference when the product is operated in a commercial environment This product generates uses and can radiate radio frequency energy and i...

Страница 3: ...on the stage 11 2 When using the optional stage unit 11 3 When using the high container Procedures to attach detach the condenser portion 12 3 3 Observation methods Bright field observation Phase con...

Страница 4: ...ocusing on the specimen 16 9 Moving the specimen 16 3 5 Recording with the camera 17 1 Changing between the visual light path and the camera light path 17 2 Focusing on the specimen 17 3 Acquiring the...

Страница 5: ...ption 22 4 9 Operating procedures of CKX3 SLPIC option 23 4 10 Selecting procedures of the specimen holder option 23 4 11 Selecting procedures of the fluorescence mirror unit including options 24 1 Se...

Страница 6: ...t Phase contrast standard set 40 7 7 Attaching the filter slider for transmitted light illumination 41 7 8 Attaching the light source for reflected light illumination Fluorescence set 41 7 9 Attaching...

Страница 7: ...ation of living cells or of specimen taken from tissues to gain physiological or morphological information at hospitals or laboratories Typical field of applications are genetics human blood and tissu...

Страница 8: ...loves etc or clean the product before operation Follow the procedures described in chapter Handling Precautions Follow the procedures described in chapter Handling Precautions page 5 prior to using th...

Страница 9: ...ct or from the power outlet Install the product at a location where you can reach the power cord connector or the power outlet at hand to remove the power cord quickly This product complies with the e...

Страница 10: ...bols CAUTION Mercury lamp housing Keep the AC adapter the power cord and other cables well away from the mercury lamp housing If the AC adapter the power cord and other cables contact a hot area of th...

Страница 11: ...ay be damaged if excessive force is applied Never disassemble any part of the product Otherwise failure could be caused NOTE NOTE NOTE Handling Precautions Maintenance and Storage Maintenance 1 Do not...

Страница 12: ...t until the lamp housing is cooled down sufficiently and keep it covered with a dust cover during storage 2 Before disposing of this product be sure to follow the regulations and rules of your local g...

Страница 13: ...jective For details see page 39 Phase contrast slider CKX3 SLP IX2 SL Fluorescence illuminator CKX3 RFA Reflected light illumination unit U HGLGPS U LLG150 300 U LLGAD Camera DP22 DP73 Camera adapter...

Страница 14: ...luded in the Bright field set Not included in the Bright field set and Phase contrast entry set Diopter adjustment ring a Stage center plate Brightness adjustment knob Main switch 1 Aperture iris diap...

Страница 15: ...plate 2 Field iris diaphragm centering knob Field iris diaphragm lever 8 Filter slider for reflected light illumination 5 Burner centering knobs Mirror focusing screw Back of lamp housing Camera Adapt...

Страница 16: ...hase contrast entry set and Phase contrast standard set you can move the microscope by raising the base portion front a and sliding the back side c of the microscope frame CKX53S 1 2 3 NOTE TIP 2 Atta...

Страница 17: ...en holder and mechanical stage CKX3 MVR Place the container on the specimen holder option For containers and specimen holders corresponding to containers see page 23 Place the specimen holder option o...

Страница 18: ...e brightness not so dazzling 1 TIP 2 2 Changing between bright field observation and phase contrast observation Phase contrast entry set Phase contrast standard set Fluorescence set When performing th...

Страница 19: ...tion depending on containers or specimens 1 TIP 4 Selecting the objective Rotate the revolving nosepiece a to engage the objective b with the magnification to be used for observation in the light path...

Страница 20: ...of the brightness adjustment knob Rotating the brightness adjustment knob in the front direction arrow direction makes the brightness brighter and rotating it in the opposite direction makes it darke...

Страница 21: ...cury vapor has cooled down and liquefied Turn ON the main switch after 10 minutes For details refer to the instruction manual for each power supply unit The discharge type mercury burner may not be ig...

Страница 22: ...e filter slider for reflected light illumination and engage the empty hole of the slider in the light path To adjust the brightness hold and slide the knob a of the filter slider for reflected light i...

Страница 23: ...e following illustrations show the image acquisition areas of camera adapters compared with observation area by the eyepieces field number 22 1 TIP Refer to page 14 If the focusing of the image throug...

Страница 24: ...mirror unit from attaching dust 1 1 2 2 TIP 2 Turning OFF the light source mercury burner Fluorescence set Turn OFF the main switch of the power supply unit For details refer to instruction manuals f...

Страница 25: ...nd rotate coarse fine focusing knobs to bring the specimen into focus Change to the low magnification objective e g 10X While looking into the right eyepiece with your right eye rotate the right diopt...

Страница 26: ...st the space according to the size of the container to be placed on the stage if necessary Loosen the umbra shield knob a Then move the umbra shield along with the umbra shield column to adjust the po...

Страница 27: ...f the objective with correction collar the objective cannot exhibit its performance When using the objective with correction collar adjust the correction collar Objective with correction collar LUCPLF...

Страница 28: ...ontrast slider see page 40 Rotate the knurled portion of the centering telescope to focus on the phase contrast ring slit image b Insert the centering knobs d to the centering screw holes 2 positions...

Страница 29: ...the objective PLCN10X or CACHN10XIPC in the light path 1 TIP 2 c d 4 10 Selecting procedures of the specimen holder option The container size differs depending on each container maker For the size of...

Страница 30: ...contains the B excitation fluorescence mirror unit and the G excitation fluorescence mirror unit as a standard mirror unit In addition to the B excitation or the G excitation fluorescence mirror units...

Страница 31: ...hase contrast slider in the light path 12 The umbra shield ambient light shielding plate is engaged in the light path Remove the umbra shield from the light path 15 3 The image is poor The contrast of...

Страница 32: ...Eliminate the fog The objective or the filter is dirty Clean the objective or the filter 5 The field iris diaphragm is not adjusted appropriately Adjust the field iris diaphragm until its image circu...

Страница 33: ...large aperture 5 The mechanical stage CKX3 MVR does not work smoothly The fixing knob is loosened Tighten the fixing knob firmly 38 The specimen is heavy Place the light specimen on the stage and con...

Страница 34: ...ary distance adjustment range 48 mm 75 mm Eyepiece tilt angle 45 Light path split ratio Visual light path 0 Camera light path 100 Visual light path 100 Camera light path 0 Camera port Olympus camera a...

Страница 35: ...ptions iPC aperture common in 4X 10X 20X and 40X Empty hole Options 45 mm 2 pieces Fluorescence illuminator CKX3 RFA Fluorescence mirror unit slider Number of selectable positions 3 positions Applicab...

Страница 36: ...LN20XPH Applicable slider IX2 SL PH2 phase contrast ring IX2 SLPH2 Applicable objective LUCPLFLN40XPH Applicable slider IX2 SL Specimen holder Dimensions 128 mm x 86 mm Dimension of container holding...

Страница 37: ...icable burner SHI 130OL Burner capacity 130 W Average burner service life 2000 hours Rated input 100 240 VAC 50 60 Hz 2 0 A Liquid light guide U LLG150 length 1 5 m U LLG300 length 3 m Light guide ada...

Страница 38: ...Stage center plate Objective Filter for transmitted light illumination Filter holder for transmitted light illumination Optical element ring Fluorescence mirror unit Filter slider for reflected light...

Страница 39: ...ibed on the glass stage plate are readable from the top 1 TIP 1 Attaching the condenser portion Loosen the condenser fixing knob a to the extent that it does not fall off Hold the condenser diagonally...

Страница 40: ...the side of the fluorescence mirror unit and push it in the arrow direction until the fluorescence mirror unit touches the end In this case attach the fluorescence mirror unit at 2 positions as shown...

Страница 41: ...r clamping screws 2 positions a of the microscope frame using the Allen wrench and remove the dust cover b 1 2 3 4 Loosen the setting screws 2 positions c of the illuminator using the Allen wrench and...

Страница 42: ...crew the adjuster portion l of support pillar for fluorescence illuminator into the screw hole of the column portion i until the screw becomes slightly visible 9 10 11 12 After the light source side p...

Страница 43: ...ated If you want to attach the removed attenuator filter c back to the previous position be sure to place the at tenuator filter c on the attenuator filter which was used alone At this time face the s...

Страница 44: ...he umbra shield column to adjust the height Decide the height and tighten the umbra shield knob a firmly The lower the position of the umbra shield is the more the ambient light can be blocked Adjust...

Страница 45: ...a c b 1 For objectives provided with each set see page 29 For specifications of objectives including options see page 30 It is recommended to attach the objectives as shown in the picture where the r...

Страница 46: ...set Phase contrast standard set 3 Attaching the phase contrast ring option In case of the centering phase contrast slider IX2 SL the phase contrast ring to be used for the phase contrast observation d...

Страница 47: ...he burner clamping screws c 2 pcs Attach the positive pole of the specified mercury burner d to the fixed mount on the upper side then the negative pole to the mount on the lower side Be sure to use t...

Страница 48: ...of the power supply unit to 0 0 For details refer to the instruction manual for the power supply unit Burner service life CAUTION When removing the mercury burner follow the procedures opposite to tho...

Страница 49: ...paper surface to light TIP 1 2 3 4 4 Centering the mercury burner only when combined with the mercury lamp housing U LH100HG The mercury burner emits the light by means of discharge produced when a c...

Страница 50: ...eft half of the field B in the picture Insert the Allen screwdriver in the mirror focusing screw on the rear of the mercury lamp housing and turn it to bring the mirror arc image e into focus C in the...

Страница 51: ...nd put the fixing knob through the hole of the cable holder Then attach clamping knobs to each stage again and attach each stage to the plain stage For procedures to attach the stage to the plain stag...

Страница 52: ...nty period are paid services If you have any questions please contact Olympus Check results Date Check items 1 More than 8 years have passed since original purchase of the illumination device or the t...

Страница 53: ...ing portions indicate operating portions described in 2 1 Bright field set Phase contrast entry set Phase contrast standard set page 8 TIP Press the main switch of the microscope frame Place the speci...

Страница 54: ...rescence observation procedures 9X axis knob page 16 aY axis knob page 16 page 47 2Umbra shield page 15 Place the specimen on the stage Engage the fluorescence mirror unit suitable for the specimen in...

Страница 55: ...r cord which is approved by one of the agencies mentioned in Table 1 please use replacements approved by any other equivalent and authorized agencies in your country Country Agency Certification mark...

Страница 56: ...Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Materielkontrollanstalter SEMKO HAR 10 10 50 sterreichischer Verband f r Elektrotechnik VE VE HAR 30 10 50...

Страница 57: ...Memo...

Страница 58: ...Memo...

Страница 59: ......

Страница 60: ...ower 446 Bongeunsa ro Gangnam gu Seoul 06153 Korea Distributed by Manufactured by 48 Woerd Avenue Waltham MA 02453 U S A Shinjuku Monolith 2 3 1 Nishi Shinjuku Shinjuku ku Tokyo 163 0914 Japan www oly...

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