5
PHASE CONTRAST
1.
Remove microscope cover.
2.
Turn on the halogen light switch (
1
) for transmitted light to “I” (ON).
3.
Check the light path. The light path selector knob (
2
) should be pushed in all the way.
4.
Adjust the light intensity using the brightness adjustment knob (
3
). The numerals to the right
of the lamp voltage indicator LEDs (
4
) indicate the voltage.
5.
Adjust the interpupillar distance. While looking through the eyepieces, adjust oculars (
5
)
until the left and right fields of view coincide completely.
6.
Make sure the 10x phase objective (
6
) is in place (phase objective has a ‘Ph’ engraved on it).
7.
Place the slide with your specimen on the stage (
7
) and hold it with the specimen holder (
8
).
8.
Find your specimen using the stage controls (
9
).
9.
Focus specimen using fine/course focussing knobs (
10
).
10.
Adjust the diopter:
-
Close your left eye and focus on the specimen using the fine focus knob.
-
Close your right eye and focus on the specimen using the diopter ring (
11
) on the left
ocular.
-
Open both eyes and confirm that the focus is comfortable.
11.
If desired switch to the next phase objective by rotating the nosepiece (
12
) and focus.
12.
Focus the light on the specimen:
-
Close field iris diaphragm (
13
) until you can see the edges.
-
Focus the image of the field iris diaphragm by raising or lowering the condenser
using the condenser height adjustment knob (
14
).
-
Check if the circle of light is centered in the field of view. If not, use the two
condenser centering screws (
15
) to move the field iris diaphragm image to the center
of the field of view.
-
Open the field iris diaphragm until its image circumscribes the field of view.
22.
Open the condenser aperture iris diaphragm (
16
) all the way.
23.
Rotate the condenser turret
(23)
to engage the phase contrast ring until the visible marking
matches the ‘Ph’ mark on the respective objective.
24.
While looking through the oculars, increase the intensity
(3)
until you can see clearly.
25.
Remove one of the oculars and look down the tube. A bright and a dark ring can be seen. If
the rings do not exactly overlap (most likely they will), use the optical element centering
screws
(24)
to center the phase contrast ring so that the bright ring overlaps the dark ring
within the field of view.
26.
Replace the ocular.
27.
Examine specimen and document image if necessary:
-
If using 35mm film, use checklist for ‘
Olympus 35mm Camera’
.
-
To obtain still digitized images, use checklist for ‘
Polaroid Digital Microscope
Camera (DMC).
-
To obtain still digitized images and digitized movies, use checklist for ‘
Sony, color
Video camera’.
This Manual: http://www.manuallib.com/file/2605418
