9
SIMULTANEOUS FLUORESCENCE AND PHASE CONTRAST
1.
Remove the microscope cover.
2.
To turn on the mercury bulb, set the main switch
(18)
on the Power Supply Unit to “I”(ON).
The light indicator
(19)
on the Power Supply Unit should go ON. Wait 5 to 10 minutes for
the mercury bulb to stabilize. The mercury bulb should stay on for at least 15 minutes before
turning off.
3.
Setup the microscope for Phase Contrast imaging (see instructions on ‘
Phase contrast
’
page
5, #2-26).
4.
Engage the fluorescence mirror unit
(17)
until the proper filter cube is in place:
-
WU – #1, dichroic mirror DM400, excitation filter BP330-385, barrier filter BA420
-
WB – #2, dichroic mirror DM500, excitation filter BP450-480, barrier filter BA515
-
WG – #3, dichroic mirror DM570, excitation filter BP510-550, barrier filter BA590
5.
To allow light from the mercury bulb to reach the specimen, slide the shutter knob
(20)
to
position marked
™
.
6.
Adjust the transmitted light for the best balance of fluorescence and phase contrast brightness
using the brightness adjustment knob
(3)
.
7.
To improve image contrast and to prevent color fading of fluorescent light in other part than
observed region, pull out the field iris diaphragm knob
(21)
so that the image of the field iris
diaphragm just circumscribes the field of view.
8.
To help adjusting the brightness of you the observed image and to improve the contrast, pull
out the aperture iris diaphragm knob
(22)
, the aperture iris diaphragm will get smaller.
9.
Examine specimen and document image if necessary:
-
If using 35mm film, use checklist for ‘
Olympus 35mm Camera’
.
-
To obtain still digitized images, use checklist for ‘
Polaroid Digital Microscope
Camera (DMC).
-
To obtain still digitized images and digitized movies, use checklist for ‘
Sony, color
Video camera’.
10.
To avoid bleaching, slide the shutter knob
(20)
to the position marked
˜
whenever you are
not observing the specimen.
11.
When finished:
-
Slide the shutter knob
(20)
to the position marked
˜
.
-
If mercury bulb has burned for at least 15 minutes, turn off the mercury bulb by
setting the main switch
(18)
on the Power Supply Unit to “O”(OFF). The light
indicator
(19)
on the Power Supply Unit should go out.
NOTE:
The mercury bulb needs to cool down for at least 15 minutes before turning
back on.
-
Lower the stage by turning the focus knob
(10)
towards you and remove the slide
with your specimen from the stage
(7)
.
-
Turn the nosepiece (
12
) back until the 10x objective for brightfield imaging is into
place.
-
Push the field iris diaphragm knob
(21)
and the aperture iris diaphragm knob
(22)
al
the way back into the microscope.
-
Engage the fluorescence mirror unit
(17)
back to BF (brightfield) - #6
-
Rotate the condenser turret
(23)
until the BF (brightfield) mark is visible.
-
Turn the light intensity down using the brightness adjustment knob (
3
).
This Manual: http://www.manuallib.com/file/2605418
