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Dichroic Mirrors and Filter Configurations of Fluorescence Mirror Units

Excitation

Method

Mirror Unit

Dichroic Mirror

Excitation Filter

Barrier Filter

Fluorochrome

U-MWU2

U-MNU2

DM400

BP330-385

BP360-370

BA420

· Autofluorescence observation
· DAPI staining: DNA
· Hoechest 33258, 33342 staining: Chro-

mosome

U-MNV2

DM455

BP400-410

BA455

· Catechrolamine observation
· Serotonin observation
· Tetracyine staining: Bones, teeth

U-MWBV2

U-MNBV2

DM455

BP400-440

BP420-440

BA475

· Quinacine & quinacine mustard stain-

ing: DNA

· Thioflavine S staining: Lymphocite
· Acriflavine staining: Nucleic acid
· CFP

U-MWB2

BP460-490

U-MNB2

DM500

BP470-490

BA520IF

U-MSWB2

BP420-480

· FITC staining: Fluorescent antibody
· Acridine orange staining: DNA, RNA
· Auramine staining: Tubercle bacteria
· EGFP, S65T, RSGFP

U-MWIB2

U-MNIB2

DM505

BP460-490

BP470-490

BA510IF

U-MWG2

BP510-550

U-MNG2

DM570

BP530-550

BA590

U-MSWG2

BP480-550

· Rhodamine & TRITC staining: Fluores-

cent antibody

· Propidium iodide staining: DNA
· RFP

U-MWIG2

DM565

BP520-550

BA580IF

U-MWIY2

DM600

BP545-580

BA610IF

Texas Red staining: Fluorescent antibody

Color Separation Filter Combinations

U-MNUA2

DM400

BP360-370

BA420-460

For observing only the U-excitation stain,
when using U-excitation stain together
with FITC.

U-MWIBA2

U-MNIBA2

DM505

BP460-490

BP470-550

BA510-550

For observing only the B-excitation stain,
when using B-excitation stain together
with FRITC.

Meaning of Mirror Unit Name

U - M N I B A 2

For color separation

Excitation (U, UV, BV, B, IB, G, IG or IY)

Bandwidth (SW: Superwide band. W: Wide band. N: Narrow band.)

Mirror unit

Universal

Model number

Содержание BX-RFAA

Страница 1: ...urself fully with the use of this system we recommend that you study this manual thoroughly before operating the system Retain this instruction manual in an easily accessible place near the work desk...

Страница 2: ...UIDE 6 SPECTRAL CHARACTERISTICS OF FILTERS 7 SPECIFICATIONS 4 5 6 7 8 14 15 16 17 18 21 22 1 General Precautions for Observation 8 2 Selecting of the Fluorescence Mirror Unit 8 9 3 Objectives for Vari...

Страница 3: ...35 PROPER SELECTION OF THE POWER SUPPLY CORD 36 37 8 OPTIONAL MODULES 23 28 1 6 Position Filter Slider U RSL6 23 24 2 6 Position Barrier Filter Slider U RSL6EM 25 3 Rectangular Field Stop Unit BX RFS...

Страница 4: ...ed please select the proper power cord by referring to the chapter PROPER SELECTION OF THE POWER SUPPLY CORD at the end of this instruction manual If the proper power cord is not used product safety a...

Страница 5: ...ily accessed and unplugged in case of emergency 2 Maintenance and Storage 1 Clean all glass components by wiping gently with gauze To remove fingerprints or oil smudges wipe with gauze slightly moiste...

Страница 6: ...tion manual The following symbols are used to set off text in this instruction manual Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and or da...

Страница 7: ...plate Mirror unit indication pockets Page 31 x 6 Field iris diaphragm knob Page 11 Aperture iris diaphragm knob Page 12 Collector lens focusing knob Page 13 Burner centering knobs Page 13 Mirror focus...

Страница 8: ...ate an original fluorescence mirror unit it is recom mended to use the U MF2 empty mirror unit which does not include filter page 32 A blank indication stickers are provided with the illuminator Use t...

Страница 9: ...stabilizes 5 to 10 minutes Main switch P 10 Place the specimen on the stage Specimen holder X Y axis knobs Engage the mirror unit matching the speci men MU Mirror Unit Right or MU Mirror Unit Left bu...

Страница 10: ...st it near your microscope Mercury Lamp Housing U LH100HGAPO U LH100HG Motorized Reflected Fluorescence Illuminator BX RFAA Motorized Revolving Nosepiece Microscope BX61 BX62 etc Control Box BX UCB Ha...

Страница 11: ...deteriorate In such a case slightly reduce the excitation light intensity to slow color fading down and improve the fluorescence image To reduce the excitation light intensity use ND filters or apertu...

Страница 12: ...ne orange staining DNA RNA Auramine staining Tubercle bacteria EGFP S65T RSGFP IB U MWIB2 U MNIB2 DM505 BP460 490 BP470 490 BA510IF U MWG2 BP510 550 G U MNG2 DM570 BP530 550 BA590 U MSWG2 BP480 550 Rh...

Страница 13: ...0 minutes after ignition The mercury burner may not be ignited from the beginning due to variance between individual products In this case set the main switch to OFF wait for 5 to 10 seconds then set...

Страница 14: ...in the two field iris diaphragm centering screws and adjust them so that the field iris image comes at the center of the field of view 7 While pushing in the field iris diaphragm knob enlarge the fie...

Страница 15: ...Pull out the aperture iris diaphragm knob to minimize the aperture iris diameter 8 Pull out the field iris diaphragm knob to minimize the field iris dia phragm Now the aperture iris image should be vi...

Страница 16: ...to the center of field of view 4 Engage the empty place the objective cap should be removed in the light path 5 Pull out the aperture iris diaphragm knob to minimize it and push in the field iris diap...

Страница 17: ...centering of the mirror arc image 8 Using the ND Filters Fig 5 Specimen color fading can be delayed by reducing the excitation light intensity with ND filters Use the ND filters as far as they do not...

Страница 18: ...r reflected fluorescence to be effective in the simultaneous observation insert the analyzer U AN or U AN360 3 into the analyzer insertion slot above the dichroic mirror on the illuminator Do not inse...

Страница 19: ...ey circumscribe field of view Fluorescence mirror unit in use does not match specimen Use fluorescence mirror unit match ing specimen c Field of view is obscured or not evenly illuminated Objective is...

Страница 20: ...cted Connect firmly e Hand switch or PC indicates that shutter is released but light is not incident or image is cut off Shutter is moved due to external vibra tions Try perform shutter operation agai...

Страница 21: ...BV excitation Wide band 5 BV excitation Narrow band 6 B excitation Wide band U MWU2 U MNU2 U MNV2 U MWBV2 U MNBV2 U MWB2 Transmittance Transmittance Transmittance Wavelength nm Wavelength nm Waveleng...

Страница 22: ...B excitation Wide band 10 IB excitation Narrow band 11 G excitation Wide band 12 G excitation Narrow band U MNB2 U MSWB2 U MWIB2 U MNIB2 U MWG2 U MNG2 Transmittance Transmittance Wavelength nm Wavelen...

Страница 23: ...and 16 U excitation for color separation Narrow band 17 IB excitation for color separation Wide band 18 IB excitation for color separation Narrow band U MSWG2 U MWIG2 U MWIY2 U MNUA2 U MWIBA2 U MNIBA2...

Страница 24: ...m having a specific wavelength is selected from a wide spectrum of wavelengths The five major peaks of luminance are at wavelengths of 365 366 404 7 435 546 1 and 577 0 579 1 nm In addition light beam...

Страница 25: ...Available observations Reflected fluorescence Reflected fluorescence Transmitted light DIC Reflected fluorescence Phase contrast Transmitted light Applicable microscope frames BX41 BX51 BX52 BX61 BX6...

Страница 26: ...mounted filters include an exciter filter insert it so that the arrow inscription on the side faces down a a Filter with thickness of 4 mm or more Place each filter so that it fits in side the holder...

Страница 27: ...e rear panel is used avoid using the interference type or color glass type filters This is because the 6 position filter slider near the rear panel is one of the positions where the energy from the li...

Страница 28: ...ially available round stickers in the same way as with the U RLS6 filter slider 4 Gently insert the filter slider from the right of the analyzer insertion slot on the illuminator and attach the slider...

Страница 29: ...op into the position of the field iris diaphragm then tighten the clamping screw Operation Procedure 1 Insert the provided adjustment knobs into the two adjustment screw holes near the front panel and...

Страница 30: ...he Allen screwdriver Operation Procedure Observing a Double Stained Specimen 1 Set up normal reflected fluorescence observation 2 Mount the fluorescence mirror units for double staining and engage the...

Страница 31: ...luorescence of shorter wavelengths near blue Observing a Triple Stained Specimen The operation is basically similar to the double stained specimens but fluorescence mirror units for triple staining sh...

Страница 32: ...ator is used only for clamping the screws inside the illuminator To retain the performance have your local Olympus representative conduct this work NOTES Be sure to insert the sliders in the orientati...

Страница 33: ...volving nosepiece as far as it will go to connect it to the motorized revolving nosepiece connector U D6REO P5REMC Remove the screw which has been clamped at the factory using a Phillips screwdriver T...

Страница 34: ...ighten the clamping screw firmly If the clamping screw is loose the turret will be unable to be rotated due to interference with the turret cover 4 Check the mount dovetail number and place upside dow...

Страница 35: ...unit frame Dimensions of Optical Parts Barrier filter Excitation filter Dichroic mirror 26 0 1 0 3 x 38 0 1 0 3 mm thickness 1 0 05 mm Diameter 25 0 1 0 2 mm max thickness 6 mm Barrier filter commerc...

Страница 36: ...the two burner holding screws 4 Attach the positive pole of a specified mercury burner to the fixed mount on the upper side then the negative pole to the mount on the lower side Be sure to use the US...

Страница 37: ...air the safety f the equipment replace the burner when it has been used for 200 USH102D or 300 HBO103W 2 hours The burner may crack if used beyond the specified life time 2 Before replacing the burner...

Страница 38: ...he input power supply match the requirements inscribed on the rating plate The 100 V system can be used with voltages in the 100 to 120 V range and 200 V system can be used with voltages in the 220 to...

Страница 39: ...e coupling Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table...

Страница 40: ...thority of Ireland NSAI Norges Elektriske Materiellkontroll NEMKO Asociacion Electrotecnica Y Electronica Espanola AEE Hellenic Organization for Standardization ELOT Instituto Portugues da Qualidade I...

Страница 41: ...MEMO...

Страница 42: ...buya ku Tokyo Japan Postfach 10 49 08 20034 Hamburg Germany 2 Corporate Center Drive Melville NY 11747 3157 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 2 8 Hondura...

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