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H201-MINI

User Manual

April 2015

www.oko-lab.com

Connection of the Gas Supply

A single silicon tubing carries output gas from the Okolab Gas Controller to the H201-MINI. Silicon tubing connects to a

gas input - brass opening - located on a corner of the H201-MINI. See Figure 11. Connect by gently pushing silicon tub-

ing onto brass opening.

Gas Input

Figure 11.Connection with gas supply.

Working with Perfusion

The Chamber H201-MINI features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2.5 mm in

outer diameter. Small screws plug the perfusion holes when not in use. (

screws plug M3x3 THD

). Remove small screws

plug as necessary before introducing perfusion tubing.

Figure 12 shows location of perfusion holes.

1.Frontal view

2.3D view

Perfusion dedicated opening

Figure 12. Perfusion

Содержание H201-MINI

Страница 1: ...ual April 2015 www oko lab com Page 1 H201 MINI Compatible with the following XY stages Compatible with the following Okolab Controllers Physik Instrumente P 737 1SL 2SL 5SL It fits in any MW plate holder H201 T BL PLUS ...

Страница 2: ...s 4 3 Insertion of the Sample Feedback Temperature Sensor 4 4 Insertion of Sample Holder into Chamber 5 5 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 5 6 Working with 1x3 and 1x2 chamber slides magnetic locks 7 7 Connection of the Gas Supply 8 8 Working with Perfusion 8 9 Connection of the Chamber with XY stage 9 ...

Страница 3: ...onents and dimensions H201 MINI includes the following components Chamber main body Glass lid Distance between focal plane and upper side of the glass lid Chamber top view Lid Chamber Base Sample Holder Order Separately Figure 1 H201 MINI Components and Dimensions ...

Страница 6: ...ock NOTE Spacing rings are included with sample holder Grainer Petri35x10 Ibidi μ Dish35mm low Corning 35x10mm MatTek P35G X 14 X Willco 35x10 BD Falcon 35x10 Figure 7 Ring selection scheme for 35 mm dish MatTek P50G X 30 X Grainer Petri 60x15 Willco60x15 BD Falcon 60x15 Ibidi μ dish 50mm low Corning 60x15mm Figure 8 Ring selection scheme for 60 mm dish ...

Страница 7: ...agnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 9 Magnetic lock for 1 x 3 chamber slide 1 3 2 Figure 10 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 ...

Страница 9: ...fix the chamber on the stage follow the steps shown in Figure 13 1 Place the chamber on the stage See Image 1 of Figure 13 2 Tilt the chamber and slide it towards the spring until it snaps in place See Image 2 of Figure 13 3 Image 3 of Figure 13 shows the chamber installed on the XY Stage Springs 1 2 3 Figure 13 Connection of the Chamber with XY Stage ...