NuAire NU-5810, Руководство по эксплуатации и обслуживанию, Страница: 3 / 64

OM0246 Page 3 of 64
Rev 5 June/2020
DHD Direct Heat
Invitrocell CO
2
Incubator
Models: NU-5810, NU-5820, NU-5831, NU-5841
NU-5810E, NU-5820E, NU-5831E, NU-5841E
Operation and Maintenance Manual
1.0 General Description
The NuAire Direct Heat Decon (DHD) Automatic CO
2
Direct Heat Incubator has been designed to provide a reliable
controlled
In-vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage
and preservation of gametes and animal tissue cell cultures intended for research at or near body temperature.
There are five parameters that contribute to optimum growth conditions, they are:
Humidity
Precise temperature control
Precise CO
2
control
Sterility
Reliability
Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with
matching computer technology, precise temperature control, and CO
2
gas control system combining state-of-the-art
technology with years of design, quality, and manufacturing experience.
1.1 Incubator Chamber
The design and size of the DHD inner chamber provides a large capacity (5.7 cu. ft.), and ease of use. The chamber walls are
directly heated by physically attached foil heating elements on the sides, bottom, top, and back of the chamber, providing
a temperature uniformity of +0.3
°
C. A high-density insulation that has a high "R" rating covers the complete outer surfaces
of the Incubator inner chamber.
1.2 Incubator Closed loop chamber sample circulation system & HEPA Filter
A continuous operating air pump draws environmental sample from the chamber and circulates it through a HEPA filter
capsule then through sensors that measure CO
2
volume (as a percentage) in the chamber for monitoring and control.
1.3 Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer based control system specifically designed to
service the precise control requirements of the chambers environment, providing optimum programmable conditions for
culture growth. The microcomputer is “user-friendly” and provides a 5x7 LCD screen which provides operating control
parameters, status indicators, additional key operational parameters, and an imbedded touch panel to permit efficient
operator entry of data.
The microcomputer is supported with Read Only Memory (ROM) containing executable software, Random Access Memory
(RAM) for temporary storage, and Electronically Erasable Programmable Read Only Memory (EEPROM) for control set
points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power
interruption (power fault tolerant).
1.4 Incubator CO
2
Control
The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO
2
induction sensor.
The amount of energy received at the detector is an approximate logarithmic function of the CO
2
concentration in the gas
between source and detector. The wavelengths used are absorbed only by CO
2
making the measurement insensitive to
other components, such as water vapor. Detector linearization is performed with 32 bit digital accuracy. Advanced design
provides a very stable output minimizing drift and requiring less frequent calibration. The output is digital, alleviating errors
brought about by analog signals. Calibration of this control is accomplished by a zero & span calibration done on the sensor
and a display off- set calibration. These calibrations are accessible through the NuTouch LCD located on the front of the
unit.