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OM0246

Page 1 of 64

Rev 5 June/2020

Air-Jacketed Automatic CO

Incubator

Models

NU-5810, NU-5820, NU-5831, NU-5841

NU-5810E NU-5820E, NU-5831E, NU-5841E

Operation and Maintenance Manual

June 2020

Revision 5

Series 12

For 115 Vac, 50/60 Hz Only

Manufactured By:

NuAire, Inc.

2100 Fernbrook Lane

Plymouth, MN 55447

Toll-Free: 1-800-328-3352

In Minnesota: (763)-553-1270

Fax: (763)-553-0459

Содержание NU-5810

Страница 1: ...810 NU 5820 NU 5831 NU 5841 NU 5810E NU 5820E NU 5831E NU 5841E Operation and Maintenance Manual June 2020 Revision 5 Series 12 For 115 Vac 50 60 Hz Only Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In Minnesota 763 553 1270 Fax 763 553 0459 ...

Страница 2: ...eter Heater Adjustments Affecting Temperature Calibration 9 3 Setting Air Injections 9 4 CO2 Calibration 9 5 RH Calibration 9 6 O2 Calibration Section No 10 Maintaining Your DHD Invitrocell 10 1 Heated Decon Cycles 10 2 Chemical Decontamination 10 3 Power Loss recovery mode 10 4 Shutting down the incubator 10 5 Automatic Filter Change Notification Section No 11 Error Indicators Troubleshooting Sec...

Страница 3: ...ors that measure CO2 volume as a percentage in the chamber for monitoring and control 1 3 Incubator Control Electronics The NuAire Incubator Control Electronics is a state of the art microcomputer based control system specifically designed to service the precise control requirements of the chambers environment providing optimum programmable conditions for culture growth The microcomputer is user f...

Страница 4: ...ns are settable by the user to help control condensation See section 8 8 for this option Models NU 5820 and NU 5841 use a humidity monitoring control system for chamber humidity the system is external to the chamber The chamber air is routed through the water vapor saturated air in an evaporator box to add humidity when the RH sensor indicates that more humidity is required maintain a set point 1 ...

Страница 5: ... CAUTION Safety alert symbol indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury WARNING Note Biohazard Hazardous Gases Personal Protection Equipment Required Chemical Hazard Flammable Hazard Lead Free CAUTION used without the safety alert symbol indicates a potentially hazardous situation which if not avoided may result in property damage Potent...

Страница 6: ...er Most electronics motors pumps and valves are fully accessible from the back of the unit Access to the filters and CO sensor through the front of the incubator A CO2 sample port is provided on the front panel just below the door to check the concentration of CO2 in the chamber The CO2 percentage is controlled by a solid state gas infrared sensor using a single light source with dual wave length ...

Страница 7: ...rformance Parameters Temperature Control Available on All Models Performance Determined with O Set Point 21 0 Temperature Range 5 C to 55 C 37 C default 5 C above ambient to 30 C maximum ambient Temperature Uniformity 0 3 C 37 C Temperature Accuracy 0 1 C Temperature Recovery 0 12 C minute Average Temperature Display Resolution 0 1 C Temperature Sensor Type Precision Integrated Circuit Minimum Qua...

Страница 8: ...rage Note Performance ratings apply ONLY when the Incubator is installed and set up properly per the instructions in the manual has stabilized at all set points and is in a stable environment See sections 8 and 9 for proper set up While idle a system will not inject gas or RH and will not alarm 3 4 Standard Items Packed With Unit Four 4 stainless steel shelves Eight 8 stainless steel shelf bracket...

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Страница 10: ... the green bonding conductor of the supply cord and any dead metal part of the cabinet shall not exceed 0 10 ohms 4 3 Functional Tests These functional tests are performed on every Incubator over a minimum of a 48 hour burn in period The results are recorded on the final Inspection report and a copy is included with each incubator that is shipped All failures are noted corrected and any failed tes...

Страница 11: ... 6 1 Damaged Shipments 6 1 1 Terms are F O B factory unless stated otherwise Therefore it is important to check each shipment before acceptance 6 1 2 If there is visible damage the material can be accepted after the driver makes a notation on the consignee s copy of the freight bill Then an inspection must be made to verify the claim against the carrier This inspection is the basis of your filing ...

Страница 12: ...nging door Note When locating Incubators side by side they should be spaced at least 10 apart to permit one Incubator to be in a heated Decon Cycle with a minimal heating disturbance on adjacent Incubators running in their normal run mode 7 2 Leveling Prior to use the incubator should be leveled using a bubble level on a middle shelf in the chamber The Incubator should have all 4 leveling feet fir...

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Страница 14: ...he air inlet on the back of the incubator is connected to the filter by a hose at the factory and does not require installation CAUTION This is a free air supply DO NOT connect to a pressurized air source 7 6 CO2 N2 Gas Supply Setup 7 6 1 Gas and Air supply HEPA filtering is installed at the factory on the front of the incubator as illustrated in the picture Each incubator is equipped with the fil...

Страница 15: ...ave two gauges USE A TWO STAGE REGULATOR All NuAire Incubators use CO2 N2 in such quantities that precise metering of CO2 N2input pressure is important for maximum performance To connect the regulator First Open the CO2 cylinder slightly for an instant this is termed cracking the valve This will blow out dust or dirt that may have collected in the valve outlet BE SURE to keep your face away from t...

Страница 16: ...he Incubator is installed correctly connected to the power source it is rated for gas connections made properly the water pan filled the shelves in place and the unit is leveled Read section 8 and follow all instructions for setting set points at the desired value Then calibrate the Incubator control systems as explained in section 9 If the Incubator is installed and calibrated correctly it will m...

Страница 17: ...hieved through the process of water evaporation vapor water pressure from a stainless steel water pan placed on the bottom of the chamber Materials of different thermal resistance e g glass plastic do not offer sufficient thermal recovery and are not recommended for water pan usage Although some metals offer better thermal coefficients than stainless steel dissimilar metals cause electrolysis in t...

Страница 18: ...n average The control system has a 3 accuracy from any given set point 90 and below Much of the information regarding the water pan can be applied with the exception of the water pan RH setpoints exceeding 90 will limit the O2 setpoint input on 5841 models to a minimum of 2 The control system uses a solid state capacitance humidity sensor to monitor the relative humidity within the chamber If humi...

Страница 19: ...hen pressed Date and Time Air CO2 RH O2 inject indicators show when gas or RH is injecting PIDT CO2 RH O2 indicate that the system showing is in Post Inject Delay Time A heated decon Cycle display An Alarm Status Menu Maintenance Required displayed 8 3 3 Active buttons on the MAIN SCREEN Button Description Action when touched Control system description i e TEMP or CO2 Brings a pop up screen that d...

Страница 20: ...icating that is a link similar to the computer or on the internet The system settings menu screen shown below is a good example of both the Text commands and Button commands Note The Main Screen is the one exception for the above described buttons The green clock text is and is a link to adjusting the time The large white Control system measured values are also a second link to the Environmental S...

Страница 21: ...1 0 for more specifics on alarm conditions 8 3 7 Password Protection There are 2 levels of password protection 1 The permanent numerical password required for accessing the Service Settings menu screen This screen is accessed by pressing the Service Settings text Icon in the System Settings menu screen The password is and it cannot be changed or deleted The functions in the Service Settings screen...

Страница 22: ...the NuAire logo icon The Main Screen displays Power Interrupted message displayed when power is lost and then re connected current Calibrated values of enabled control systems Current operating mode run standby or Power loss recovery Door Ajar Alarms and Maintenance notices 8 4 2 Inject CO2 O2 RH Status On the bottom right of Main screen CO2 O2 RH text indicates when the inject valve is open and t...

Страница 23: ... for more details on Alarms 8 5 Rear Component Panel Detail The rear control panel contains the following functions see also BDC 15170 8 5 1 Power Cord The power cord is 6 5 foot 2 m in length type SVT molded plug allowing for long life and easy cleaning 8 5 2 Circuit Breaker All control electronics are protected with a circuit breaker that may trip at 110 of load rating but will trip at 145 of lo...

Страница 24: ...OM0246 Page 24 of 64 Rev 5 June 2020 8 6 Front Component Panel The front component panel contains the following functions BCD 18316 ...

Страница 25: ...nce Section 10 8 6 3 CO2 Supply Filter The CO2 supply filter is installed at the factory and should be changed following the instructions found in Maintenance Section 10 8 6 4 Air Inlet Filter All models The air inlet filter is installed at the factory and should be changed following the instructions found in Maintenance Section 10 This is a free air supply Note DO NOT CONNECT TO A PRESSURIZED SOU...

Страница 26: ...OM0246 Page 26 of 64 Rev 5 June 2020 8 7 Run Mode Operator Interactions ...

Страница 27: ...equired when the incubator is first turned on then to perform calibration functions and address abnormal conditions status If an abnormal condition has or does exist for a particular parameter an alarm becomes active and the Alarm Status button will appear This could be a catastrophic alarm condition which could harm the tissue culture cells It does indicate an operational abnormality and should b...

Страница 28: ...h achieving these lower O set points NU 5831 and NU 5841 8 8 1 4 Automatic Adjustment of Post Inject Delay Times when O control system is active Post inject delays are automatically adjusted according to the O set point to facilitate gas and RH recovery after a door opening The value displayed in the option menu will not change but is the basis for a formula in the software that calculates the new...

Страница 29: ... following from the Main screen Select the NuAire Logo to enter System Settings Select Service Settings and enter 9876 and then hit enter ENT In the Service Settings menu select the Diagnostics button for the desired function Output Input Diagnostic Parameter Functions Temperature 1 Safety Relay on off 2 Chamber Temp Sensor shows current temp 3 Safety Temp Sensor shows current temp 4 Chamber heate...

Страница 30: ...cent The LCD display will show phase firing percentage chosen default is 0 6 Perimeter Heater This function shows the current state of the perimeter heater This function also allows the perimeter heater to be turned on at different percentages 0 50 and 100 percent The LCD display will indicate percentage of phase firing chosen default is 0 CO2 Control Diagnostics 1 CO2 Sensor This function shows t...

Страница 31: ...Un Calibrated sensor signal 2 N2 Inject Valve on off This function indicates the on off default off state of the N2 Inject solenoid valve and allows the activation of it 3 O2 Bypass valve on off THIS FEATURE IS NOT ACTIVE 4 Blocking Solenoid on off This function indicates the on off default off state of the Blocking solenoid valve and allows the activation of it General Control Diagnostics 1 Power...

Страница 32: ...t 0 5 1 10 0 5 Post Inject Delay Time PIDT 60 seconds 0 250 Relative Humidity 1 System enable On On Off in Settings Screen 2 Maximum above Set Point 8 0 0 0 30 0 3 RH Low Time Out timer 60 minutes 0 300 4 Minimum below Set Point 3 0 0 1 15 0 5 Post Inject Delay time PIDT 90 seconds 0 300 O2 1 System Enable On On Off in Settings Screen 2 Maximum above Set Point 1 0 0 0 10 0 3 O Time out timer 30 mi...

Страница 33: ...mperature recovery cycle The delay time is required to prevent temperature overshoot of the control set point CO2 1 CO System Enable Enabled disabled Is located in the bottom right of the screen This function will enable or disable the CO system If the system is Enabled it will be displayed in the MAIN screen If it is disabled it will not be displayed In the Main or Option Settings screen for the ...

Страница 34: ...chamber O 1 System Enable Enabled disabled on models NU 5831 and NU 5841 To Access this function press Settings Text in Service Control Screen This function will enable or disable the O system If the system is Enabled it will be displayed in the MAIN screen If it is disabled it will not be displayed in the Main orthe Option Settings screen for the O system System Enabled with check mark means the ...

Страница 35: ...8 0 disables the feature View time remaining until current notice is triggered The Clear Password button that clears the 4 digit password that was input through the Set Password screen Change language Button Pressing this button brings up a screen with the language choices of English default Spanish French German Pressing the button in that screen of the language desired will change the user scree...

Страница 36: ... set screen If the date and time are correct you can press exit and go to the MAIN screen If you press Exit you will go to the main screen however if there is a power cycle the incubator will reboot to the Factory set up screen again All setup information will show correctly but you must press save to boot up into the MAIN screen after a power cycle The model number and the serial number can be ve...

Страница 37: ...g the serial number you just entered Default is blank if no number is entered Touch the Set Language text then touch the save button This sets the language on all of the user screens The language in the Service Settings menu screen will remain in English English is default language if none is chosen Touch the green save button to exit the factory screen The final change that is requested is the cl...

Страница 38: ... Do not place the glass beaker on the bottom of the chamber because it will result in a slightly higher temperature reading due to the heater located on the chamber bottom Placing the thermometer in glass beaker on the middle shelf will give the most accurate results for calibration The chamber should be humidified to avoid false low readings due to evaporation of water from the flask An accurate ...

Страница 39: ...oint being used In the door and perimeter heater adjustment screen 0 0 the calculated base duty cycle Adjusting the value up or down will increase or decrease the percent of power to each heater in 0 1 increments Small adjustments are recommended to a maximum of 5 0 at a time The following steps should be taken for setting these duty cycle percentages Allow Incubator to stabilize at its given temp...

Страница 40: ...libration check and change if necessary the Incubator in line filter found within the Sensor bay When unit has stabilized at the operational temperature and CO2 percentage take an independent measurement and if necessary perform the following In Service Settings enter the CO2 Calibrations menu Press the CO2 Offset Calibration text button Use an independent instrument to determine actual CO2 percen...

Страница 41: ... be performed after a Closed Door sensor calibration when possible if CO2 supply pressure of the Incubator is changed or is the CO2 flow control valve is disturbed The following steps should be taken for the CO2 injection calibration Enter CO2 Calibrations by means of the Service Settings menu Select the CO2 Injection Time text Follow the on screen instructions 9 5 Relative Humidity sensor calibra...

Страница 42: ...to access the key pad screen then to input the independently measured value and press the ENT button on the key pad This returns you to the Calibrate RH offset screen I might take a couple of seconds for the new value to appear in this screen Then press the SAVE button to return to the Service settings screen Pressing the exit button in that screen will return you to the MAIN screen NOTES 1 When y...

Страница 43: ...pply Filter should be replaced every fifth empty CO2 tank or when the filter is visibly discolored yellow brown Note Direction of flow IN is labeled on one side of filter when replacing filter Sensor Bay Capsule Filter P N RF566 Capsule Uni Directional In Line Wet This filter should be replaced EVERY TWO YEARS to assure optimum performance A visual check should be performed when maintenance remind...

Страница 44: ...e the instructions above for choosing this cycle in the System Settings Menu OUTER DOOR MUST REMAIN CLOSED DURING THE DOCONTAMINATION CYCLE OR IF CHAMBER TEMPERATURE IS OVER 55 THE DECONTAMINATION CYCLE WILL HEAT THE INCUBATOR INTERIOR SURFACES TO 145 C CONTACT WITH ANY SURFACE INSIDE OF THE OUTER DOOR DURING THIS CYCLE CAN RESULT IN BURNS PREPARATION Following the prompts in the incubator display...

Страница 45: ...NuTouch display screen The decon cycle will automatically go through the following phases HEAT UP 2 hours on average Complete the chamber preparation and either schedule a start time or press the Start button to start the heat up portion of the cycle Temperature display shows chamber temperature Text displays that the unit is currently in the Heating Up cycle A timer tracks how long the unit has b...

Страница 46: ... drying cool down phases will resume when power is restored The decon phase will reset to the beginning of the 3 hour cycle or if the temperature drops more than 10 it will go back to the heat up phase then resume the cycle when decon temperature is reached It is considered normal for some odor to occur during the Decon Cycles This odor reduces with use of the Decon Cycle Some discoloration due to...

Страница 47: ... left in place Empty water pan then clean it with a disinfectant then fill with 300ml of water Place it back in the chamber on the highest shelf for the decon cycle FOLLOW ALL FEDERAL STATE AND LOCAL REGULATIONS THAT APPLY TO THE DISINFECTANT USED TO CLEAN THE CHAMBER Note CO2 RH and O systems will be idle during the cycle Protection of the CO2 and O sensors during the heated cycle is automatic RH...

Страница 48: ... the humidity left from the decontamination phase Temperature display shows chamber temperature during cycle The Text displays that the Decontamination cycle is in progress A timer tracks the remaining time in Decontamination COOL DOWN 3 hours When the humidity reduction period is complete the heaters are shut off to cool the chamber to the user chosen temperature set point Temperature display sho...

Страница 49: ... meaning they will not control or alarm while in this mode o Power Loss Recovery shows in place of RUN in the main screen o Heaters and Air Pump run as normal Once entered this mode will run for 1 hour A count down timer screen seen below can be found when pressing the CO2 or O2 Diagnostics button in the Service Settings screen It is also recorded in the event log 10 4 Shutting down the Incubator ...

Страница 50: ...set the notification timer to the default of 24 months by pressing save This is recommended Change the timer by sliding the green button right to decrease or left to increase the amount of time until the next notification The number of months showing will change as the button is moved Press the Save button to save the timer setting and go the General Setting screen Pressing the Exit button will no...

Страница 51: ...il Diagnostics is entered or another alarm becomes active Step 2 CLEAR ERROR INDICATORS Error indicators can be cleared by pressing the Alarm Status button and entering the Alarms Menu and pressing the Clear All button if the error is still present the indicator will return when the alarm time reaches 0 Step 3 MONITOR REOCCURRENCE OF ERROR INDICATORS If reoccurrence of the error indicator is immed...

Страница 52: ...ture sensor Confirm it is plugged in at board connector P6 Replace CO2 shroud temperature sensor Sensor Bay Temp Low Sensor bay is below temp set point 2 See CO2 diagnostics screen Check control board output to relay replace board if faulty Check that all connections are secure Check sensor bay heater for function replace if faulty Sensor Bay Temp High Sensor bay temp is above 65 deg C Check contr...

Страница 53: ...pump and hosing Check sensor and inject solenoid for loose connections Evaporator Temp low Water temperature in the water tank is below the alarm limit for 120 minutes Check for loose connections on the tank heater Check for loose connections on the tank temperature sensor Check the tank heater for function replace if faulty O2 System Displayed Error Code Code Description Checks and Corrections O2...

Страница 54: ...e Failure A change in parameters failed to write to memory Replace Control board 12 VDC Power Failure Low voltage detected on the 12 VDC power circuit Check output Power Supply If good could be Control Bd Check power supply connection to Control board DC power supply failed replace 12 VDC Power Failure Low voltage detected on the 12 VDC power circuit Check output Power Supply If good could be Cont...

Страница 55: ...to change Filter Check and Change filters in sensor bay and reset timer Check Filter and extend timer if filter doesn t need changing Extend Timer See section 10 4 for more detail on all responses Message is RH Watertank Fill with maximum 3 liters of distilled single water no purer Than 1 Mega Ohm removes message on main screen For further assistance call NuAire Customer Service at 1 800 328 3352 ...

Страница 56: ...thstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply...

Страница 57: ...ed Component Material Incubator Cabinet Painted Steel Outer Door Painted Steel Chamber Stainless Steel Outer Door Gasket Vinyl Clad Magnet Inner Door Gasket Silicon Rubber Front Service Panel Painted Steel Electronics Panel Painted Steel Sensor Bay Aluminum Exhaust Filter Aluminum Hosing Silicon Rubber Tubing Stainless Steel Air Pump Various Steel Copper Nylon Motor Various Steel Copper PVC Printe...

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