ELC-03XS User Manual
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version 2.2
page 30
Figure 11: headstage connections,
A
: differential measurement,
B
: single-ended measurement
8.2.
Extracellular Voltage Measurement
Extracellular measurements are usually done in the loose-patch configuration or with special
metal microelectrodes. Recordings with extracellular metal electrodes is simple. The electrode
is advanced into the region where the recordings will be made using a micromanipulator and
the signals are filtered and amplified (see chapter 5 in Lalley et al., 1999 for details) as
required. For loose patch recording the basic procedure is the following (Barbour & Isope,
2000, Nunemaker et al, 2003):
Approach the cell in VC mode and apply square voltage pulses to the electrode.
Contact the cell and establish the loose-patch.
Set the MODE OF OPERATION switch to OFF.
Set the required amplification of the POTENTIAL OUTPUT.
Set the HIGHPASS FILTER to the desired corner frequency, e.g. 0.3 Hz.
Set the LOWPASS FILTER to the desired corner frequency, e.g. 3 kHz.
