ELC-03XS User Manual
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version 2.2
page 28
7.3.
Headstage with x1 / x100 current range (option)
Optionally, the ELC-03XS amplifier has a switch or TTL selectable extended current range of
x100. This is realized by reduction of the headstage feedback resistance from 100 M
to 1
M
. It enables the amplifier to generate higher currents and voltages with low resistance
electrodes, e.g. for electroporation, both in current clamp and in voltage clamp mode.
Figure 8: ELC-XS with switchable headstage, front panel view
The extended current range can be set at the front panel of the ELC-03XS with an additional
switch labeled RANGE x100 or with a TTL signal applied to the x100 INPUT (TTL) BNC
connector at the rear panel of the amplifier (see also chapter 4). If the switch is set to ON the
current range is extended to
12 µA. This mode is indicated at the headstage by an additional
LED (x100 ON, see Figure 10).
Important:
In the VC x10 range which is usually used for generating high amplitude voltage
pulses suitable for electroporation of large molecules the x100 current range is automatically
activated allowing for instance 10 V pulses with 1 M
electrodes.
Note
: All basic operations and settings (e.g. bridge balance or BIAS adjustment) should be
performed in standard x1 mode, i.e. RANGE x100 switch set to OFF, because these are also
affected by the RANGE x100 mode!
Exception
: The electrode resistance test works well in x100 mode!!
If the ELC-03XS operates in x100 high current mode, all current related signals have to be
corrected by the factor of hundred:
- CURRENT OUTPUT SENSITIVITY (V / nA): 0.001, 0.002, 0.005, 0.01, 0.02, 0.05, 0.1
- STIMULUS INPUT: 100 nA / V (CC x1) or 1µA / V (CC x10)
- Holding CURRENT / gated CURRENT: 0 to 999 nA (CC x1) or 9.99 µA (CC x10)
- CURRENT FROM HEADSTAGE: 0.001 V / nA
- CURRENT DISPLAY: shows always nA, scaling is adapted automatically.
Figure 9: Current range switch
