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II. Microscopy Method

Dark-field Microscopy under the Dia Illumination

Focus on the specimen with the bright-field microscopy under the diascopic illumination.

(See Pages 26 to 27.)

Set the microscope for the dark-field
microscopy under the diascopic illumination.

Rotate the condenser turret of the universal
condenser to the “DF” position. (See Page 55.)

Press the OBJ. switch on the operation panel and
locate the desired objective into the optical path.
(See Page 43.)

The objective of a magnification of 4x or more
and a numerical aperture of 0.7 or less is usable
to the dark-field microscopy.

Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)

Adjust the brightness with ND filters.
(See Page 44.)

Fully open the field diaphragm and the aperture
diaphragm. (See Page 54.)

Return to the bright-field microscopy under the
diascopic illumination.

Rotate the condenser turret of the universal
condenser to the “O” position. (See Page 55.)

Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)

Adjust the brightness with ND filters.
(See Page 44.)

Adjust the field diaphragm and the aperture
diaphragm. (See Page 54.)

UEPI2

USB

RS23

LCNT

ND8

NCB

F.S.

JAPAN

6 9 6

0 0 1

3 x 2 ST

AGE

JAPAN

STOP

JAPAN

DF FL1 FL2

FL1

FL2

100

OUT

LV-TT

OBJ.

CUBE

A.S.

EPI

DIA

EPI

DIA

Operation

panel

OBJ. switch

DIA brightness switch

UEPI2

USB

RS23

LCNT

ND8

NCB

F.S.

JAPAN

6 9 6

0 0 1

3 x 2 ST

AGE

JAPAN

STOP

JAPAN

DF FL1 FL2

FL1

FL2

100

OUT

LV-TT2

OBJ.

CUBE

A.S.

EPI

DIA

EPI

DIA

Operation

panel

DIA brightness switch

Attach the accessories required for the dark-field microscopy under the dia illumination.

The following accessories must be attached to perform the simplified/sensitive color dark-field
microscopy under the dia illumination.

•

Universal condenser (dry) and dark-field annular diaphragm (See Page 78 and the LV-CUD
instruction manual.), or dark-field condenser (See Page 78.)

Содержание Eclipse LV100DA-U

Страница 1: ...Universal Design Microscope UDM ECLIPSE LV100DA U Instructions M421 E 07 5 NF 1 2 3 ...

Страница 2: ......

Страница 3: ...every effort has been made to ensure the accuracy of this manual if you note any points that are unclear or incorrect contact your nearest Nikon representative Some of the products described in this manual may not be included in the set you have purchased Also be sure to read the manuals for any other products that you are using with this system If the equipment is used in a manner not specified b...

Страница 4: ...ve Use of a non conforming power line may result in equipment malfunction failure or fire 5 Power cord Be sure to use the specified power cord for the product Using a wrong power cord may result in malfunctions or fire The product is classified as subject to Class I protection against electrical shock Make sure it is connected to an appropriate ground terminal protective earth terminal To prevent ...

Страница 5: ...ess at the worst case To prevent injury follow the guidelines below 1 Place a UVC collector lens into the optical path of the microscope unless the UV excitation light is necessary On the LV UEPI2A epi illuminator an UV filter automatically enters the optical path when the microscopy method is turned to the bright field microscopy or the dark field microscopy The UV filter is removed from the opti...

Страница 6: ...nt light the image quality deteriorates Always install the product with a surrounding clear area of 10 cm or more Install the product in a location that is free from considerable dust or dirt Install the product on a flat surface with little vibration Install the product on a sturdy desk or table for the base of the microscope system Do not install the product in a hot and humid location Select a ...

Страница 7: ...on light filters which are exposed to strong light deteriorate over time Replace them depending on their total operating hours Filter characteristics may alter if the filter is exposed to high humidity To prevent changes or degradation of filter characteristics avoid using or storing the filters under conditions of high humidity or high temperature and avoid subjecting the filters to rapid tempera...

Страница 8: ...4 7 Bright field Microscopy under the Dia Illumination 26 8 Polarization Microscopy under the Dia Illumination Simplified Sensitive Color 28 9 Dark field Microscopy under the Dia Illumination 29 10 Phase Contrast Microscopy under the Dia Illumination 30 11 Differential Interference Contrast Microscopy under the Dia Illumination 32 Operation of Each Part 34 1 Power On Off 34 2 Setting Up the Micros...

Страница 9: ...ouse and Replacing Lamps 85 6 Attaching the Optical Fiber Adapter and an External Light Source 89 7 Attaching the Double Light Source Adapter 92 8 Attaching the Eyepiece Tube 93 9 Attaching Eyepieces 93 10 Attaching Objectives 93 11 Attaching the Polarizer for the Diascopic Illumination 94 12 Attaching Eye Level Risers 95 13 Attaching a Column Riser 95 14 Connecting a PC 96 15 Connecting the DS L2...

Страница 10: ... See Page 11 Coarse torque adjustment ring Binocular section Vertical tube section CAUTION for heat symbol Lamp house for the episcopic illumination 1 LV LH50PC Lamp house for the diascopic illumination LV LH50PC Filter sliders Excitation light balancer slot Part Name 1 Configuration of the Product and Control Names Front left side of the microscope This drawing depicts the ECLIPSE LV100DA U micro...

Страница 11: ...mination simultaneously connect the lamp cable of the lamp house for the episcopic illumination to an external power supply See Page 10 If the brightness of the halogen lamp is less than the desired brightness for the epi fl microscopy or so on attach an external light source equipped with a mercury lamp with the fiber adapter and the light guide fiber See Page 10 2 This part is used for the DIC m...

Страница 12: ... 0 100 IN OUT LV TT2 INTENSILIGHT C HGFIE LAMP POWER RUN TIME hrs Power cord RS 232C cable Light guide fiber External light source Intensilight C HGFIE Optical fiber adapter UEPI2 A USB RS232 C LCNT ND8 NCB F S Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S T A G E JA PA N F STOP J A P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT LV TT2 POWE R MIN MAX Power cord Lamp cable Control cab...

Страница 13: ...itch They are used to adjust the lamp brightnesses When a halogen lamp is used for the illumination its brightness can be adjusted with continuous settings When the external light source is used its brightness can be adjusted in five steps UEPI2A USB RS232C LCNT UEPI2A connector It is used to connect the LV UEPI2A epi illuminator USB connector It is used to connect a PC or the DS L2 to perform the...

Страница 14: ... lamp is on Turn off the power and allow the lamp and lamp house to cool enough before replacing the lamp Wait for at least 30 minutes after turning off the lamp Use 12V50W HALOGEN lamp only 1 2 3 LV LH50PC JAPAN 6 5 2 7 0 2 LAMP DC12V 50W ECLIPSE LV100DA U 100 240V 1 2A 50 60Hz MADE IN JAPAN including interference that may cause undesired operation This device complies with Part 15 of the FCC Rul...

Страница 15: ...unction is enabled the corresponding electrical devices are set to the predetermined conditions in accordance with the microscopy method or objective If the interlock control is disabled please be sure to operate each device manually For operations of LVSetup see LV Series Setup Tools Software Manual Motorized units can be controlled on a PC with NIS Elements the software for digital cameras when ...

Страница 16: ... to 4x objectives Condenser lens polarizer for the diascopic illumination analyzer and lambda plate for the sensitive color Universal condenser dark field annular diaphragm or dark field condenser diaphragm and objective with a numerical aperture of 0 7 or less Universal condenser Ph annular diaphragm suitable for the objective and Ph objective Universal condenser rotatable polarizer for the diasc...

Страница 17: ...age 44 5 Adjust the brightness with ND filters See Page 44 6 Move the open close lever to the upper position to fully open the field diaphragm for the episcopic illumination See Page 50 3 Set the specimen onto the stage and adjust the focus and the brightness 1 Lower the stage by turning the coarse fine focus knobs See Page 46 2 Set the specimen onto the stage 3 Turn the coarse fine focus knobs an...

Страница 18: ...ee Page 46 3 Operate the EPI brightness switch on the operation panel to adjust the brightness of the episcopic illumination See Page 42 4 Use the field diaphragm open close lever so that the field diaphragm image circumscribes the field of view See Page 50 Helpful tips It may be difficult to focus on a specimen with small contrast such on a polished surface In a case like this reduce the opening ...

Страница 19: ...eld microscopy under the epi illumination 1 Press the CUBE switch on the operation panel and light up the BF bright field position of the microscopy method indicator See Page 37 The episcopic illumination lamp is turned on with the predetermined brightness by the interlock control function and the aperture diaphragm and the field diaphragm automatically return to the previous positions The field d...

Страница 20: ...icroscopy under the epi illumination 1 Push in the analyzer slider to locate the analyzer into the optical path See Page 60 2 Push in the polarizer slider to locate the polarizer into the optical path and get the crossed Nicols position by aligning the index See Page 57 For the simplified polarization microscopy you can get the crossed Nicols position by placing the PA block LV PAB into the optica...

Страница 21: ...late slider to remove the lambda plate from the optical path See Page 62 4 Operate the EPI brightness switch on the operation panel to adjust the brightness of the episcopic illumination See Page 42 5 Adjust the brightness with ND filters See Page 44 UEPI2A USB RS232C LCNT ND8 NCB F S Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 10...

Страница 22: ...nder the episcopic illumination 1 Push in the analyzer slider to place the analyzer into the optical path See Page 60 2 Push in the polarizer slider to place the polarizer and the 1 4 lambda plate into the optical path and set the crossed Nicols position by aligning the index See Page 57 Set the polarizer to the crossed Nicols position 3 Press the OBJ switch on the operation panel to place the obj...

Страница 23: ...ilters See Page 44 5 Rotate the rotation ring of the polarizer slider to adjust the contrast See Page 57 The polarizer slider is equipped with the 1 4 lambda plate so that the contrast can be adjusted by adjusting the orientation of the polarizer 6 To perform the sensitive color microscopy push in the lambda plate slider to place the lambda plate into the optical path See Page 62 7 Adjust the EPI ...

Страница 24: ...age 60 2 Push in the polarizer slider to place the polarizer into the optical path and set the crossed Nicols position by aligning the index See Page 57 Set the polarizer to the crossed Nicols position The crossed Nicols position can be set by placing the PA block LV PAB into the optical path instead of the analyzer and the polarizer See Page 61 3 Press the OBJ switch on the operation panel to pla...

Страница 25: ...itch on the operation panel to adjust the brightness of the episcopic illumination See Page 42 6 Adjust the brightness with ND filters See Page 44 5 Set the prism selector knob of the DIC slider to the position A or B indicated on the body of the objective See Page 65 6 Rotate the prism position knob at the end of the DIC prism to set an interference color See Page 65 You can also perform the sens...

Страница 26: ...light source To prevent fading of the specimen make sure to close the shutter when you don t observe the specimen The shutter of the light source can be opened and closed with the EPI switch on the operation panel JAP AN UEPI2A USB RS232C LCNT ND8 NCB F S Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT LV TT2 OBJ C...

Страница 27: ...y method indicator See Page 37 The light source is adjusted to the predetermined light quantity and the aperture diaphragm for the episcopic illumination is adjusted to the predetermined size automatically by the interlock control function 2 Operate the EPI brightness switch on the operation panel to adjust the brightness of the episcopic illumination See Page 42 3 Adjust the brightness with ND fi...

Страница 28: ...ocate the NCB11 filter into the optical path and compensate the color temperature See Page 44 6 Adjust the brightness with ND filters See Page 44 7 Rotate the field diaphragm control toward you and fully open the field diaphragm for the diascopic illumination See Page 54 8 To use the universal condenser set the condenser turret to the O position See Page 55 9 Rotate the condenser focus knob to foc...

Страница 29: ...2 100 20 0 100 IN OUT LV TT2 4 5 Adjust the angle of the binocular eyepiece Adjust the diopter and the interpupillary distance UEPI2A USB RS232C LCNT ND8 NCB F S Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT LV TT2 OBJ CUBE A S EPI DIA EPI DIA 6 5 3 6 2 4 1 OBJ switch 1 DIA brightness switch Operation panel Chang...

Страница 30: ...4 Operate the DIA brightness switch on the operation panel to adjust the brightness of the diascopic illumination See Page 42 5 Adjust the brightness with the ND filter See Page 44 4 Return to the bright field microscopy under the diascopic illumination 1 Pull out the analyzer slider and move the analyzer away from the optical path See Page 60 2 Move the diascopic polarizer away from the optical p...

Страница 31: ... condenser turret of the universal condenser to the O position See Page 55 2 Operate the DIA brightness switch on the operation panel to adjust the brightness of the diascopic illumination See Page 42 3 Adjust the brightness with ND filters See Page 44 4 Adjust the field diaphragm and the aperture diaphragm See Page 54 UEPI2A USB RS232C LCNT ND8 NCB F S JAP AN 69 60 01 3 x 2 S TA G E JA PA N F STO...

Страница 32: ...uction manual for the LV CUD 7 Adjust the size of the field diaphragm so that the field diaphragm image circumscribes or inscribes the field of view See Page 54 UEPI2A USB RS232C LCNT ND8 NCB F S JAP AN 69 60 01 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT LV TT2 OBJ CUBE A S EPI DIA EPI DIA 5 3 2 4 7 1 6 1 Operation panel OBJ switch DIA brightness switch Note o...

Страница 33: ...position See Page 55 3 Operate the DIA brightness switch on the operation panel to adjust the brightness of the diascopic illumination See Page 42 4 Adjust the brightness with ND filters See Page 44 5 Adjust the field diaphragm and the aperture diaphragm See Page 54 UEPI2A USB RS232C LCNT ND8 NCB F S JAP AN 69 60 01 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT L...

Страница 34: ... the optical path See Page 60 2 Attach the rotatable polarizer for the diascopic illumination and rotate the upper part of the polarizer to make a crossed Nicols position See Page 59 7 5 3 7 0 1 JAPAN D D P Swing out center Thumbscrew Setscrew UEPI2A USB RS232C LCNT ND8 NCB F S 7 5 3 7 0 1 JAPAN Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S TA G E JA PA N JAP AN 69 60 01 3 x 2 S TA G ...

Страница 35: ...er to locate the lambda plate into the optical path See Page 63 The background of the field of view becomes a sensitive color The color improves the color contrast of the image and is useful for observations As the refractive index or the thickness of the specimen varies the interference color of the specimen changes UEPI2A USB RS232C LCNT ND8 NCB F S 7 5 3 7 0 1 JAPAN Achr N A 0 9 JAPAN 0 8 0 7 0...

Страница 36: ...push the power switch to the I side To turn off the product push the power switch to the side Initialization of the microscope When the product is turn on a long beep sounds the power indicator blinks and the initialization of the product starts In the initialization the product communicates with each electrical device Each device is set to the predetermined initial conditions When the initializat...

Страница 37: ... product Intensilight C HGFIE When the product is turned on the POWER indicator lights up and the LAMP indicator blinks When the product starts normally the LAMP indicator lights up EXFO X Cite 120 PC When the operation is turned on the back light of the LCD blinks When the operation starts normally the back light of the LCD lights up The external light source cannot receive any communication comm...

Страница 38: ...designated software for various settings Setup procedure To perform the setup work operate a PC and run the software the LVSetup option in LV Series Support Tools provided with this product For details about operations and setup works of LVSetup see LV Series Support Tools software manual CAUTION If you don t perform the setup work the product does not work correctly Be sure to setup the product a...

Страница 39: ...tion see 4 Illumination Switching the microscopies when the epi illumination is used To change the microscopy method operate the CUBE switch on the operation panel When the CUBE switch is pressed the filter cube turret inside the LV UEPI2A rotates and the microscopy method is changed The current microscopy method is displayed with the microscopy method indicator on the front of the LV UEPI2A OBJ C...

Страница 40: ...r a l o p e h t e c a l p n e h t d n A e h t e k a m o t h t a p l a c i t p o e h t o t n i r e z y l a n a C I D e h t t r e s n i d n A n o i t i s o p s l o c i N d e s s o r c y p o c s o r c i m C I D e h t m r o f r e p o t m s i r p e b n a c s e g a m i t s a r t n o c C I D f o t s a r t n o c e h T C I D e h t f o n o i t i s o p e h t g n i t s u j d a y b d e t s u j d a Z d o h t e ...

Страница 41: ... e d n o c l a s r e v i n U m g a r h p a i d r a l u n n a e h t t e s d n a r e s n e d n o c l a s r e v i n u e h t h c a t t A c i p o c s a i d e h t r e d n u y p o c s o r c i m d l e i f t h g i r b k r a d e h t e c a l p n e h t d n A n o i t i d n o c n o i t a n i m u l l i h t a p l a c i t p o e h t o t n i m g a r h p a i d r a l u n n a d l e i f t s a r t n o c e s a h P r e d n...

Страница 42: ...is modified from the registered condition the EPI switch or the DIA switch indicator starts blinking indicating that it is not in the registered condition This modification is referred to as User offset hereafter The condition of User offset is registered in the microscope memory therefore the post modified condition is restored when the registered combination of the microscopy and the objective i...

Страница 43: ...such its lamp cannot be frequently turned on or off Therefore when you wish to turn on or off the lamp on the light source operate the power switch on the light source Power indicator The power indicator light changes its color depending on the lighting illuminator s The indicator lights up in green when either the episcopic illumination or the diascopic illumination is lit and it lights up in ora...

Страница 44: ...rol is set to the Optional mode the light quantity is adjusted to an arbitrary value that has been set with LVSetup The interlock control is one of the functions of LVSetup For detail information see LV Series Support Tools software manual Modification from the registered condition When the light quantity is modified from the registered condition the EPI switch or the DIA switch indicator starts b...

Страница 45: ...ally adjusted to 75 of the numerical aperture of the objective Optional mode The light quantity and the aperture diaphragm for the epi illumination are set to form a diameter and a value that has been set with LVSetup Use LVSetup of LV Series Support Tools to set the interlock control For detail information see LV Series Setup Tools software manual Setting restrictions for objective switching A lo...

Страница 46: ... and color photomicrography For brightness control transmittance 25 For brightness control transmittance 6 For contrast control For interference Filter for the diascopic illumination Following two filters are mounted in the base unit of the microscope The switches for inserting and retracting the filters are provided on the right side of the microscope Lower the switch to insert the filter in the ...

Страница 47: ... GS2 G Stage 2 or the rotatable rectangular stage Rotating the stage For the circular graduated stage loosen the clamp screw on the front of the stage to turn the stage by hand For the rotatable rectangular stage loosen the clamp screw on the bottom of the stage to turn the stage by hand Centering the circular graduated stage The rotation center of the circular graduated stage must be aligned with...

Страница 48: ...stment for the coarse focus knob The rotational stiffness of the coarse focus knob can be adjusted as follows To make it stiffer rotate the coarse torque adjustment ring behind the coarse focus knob in the direction of the arrow TORQUE that is described on the microscope base To make it looser rotate the ring in the opposite direction OBJ CUBE A S EPI DIA EPI DIA Coarse torque adjustment ring Stif...

Страница 49: ...oosened by rotating it in the opposite direction of the arrow Rotate the stopper ring to reach the limit in the direction opposite from the arrow that is marked on the microscope base Example When a specimen is focused rotate the coarse focus stopper ring as far as it goes in the direction of the arrow labeled CLAMP on the microscope base The rotation for tightening is approximately three quarter ...

Страница 50: ...he binocular part and the vertical tube of the eyepiece tube are centered before the shipping so usually they can be used without an adjustment But some cameras may have their CCD centers shifted from the center of the mount part In such a case you can adjust the vertical tube center by rotating two centering screws on the back of the vertical tube 100 20 0 100 IN OUT LV TT2 100 20 0 100 IN OUT LV...

Страница 51: ...ith your left eye and the right eyepiece with your right eye to focus on the specimen 6 Repeat the steps 3 to 5 using the 50x objective and the 5x or 10x objective until the focus shift is eliminated even when the lens magnification is changed 11 Interpupillary Distance Adjustment Before performing the interpupillary distance adjustment follow the procedure described in Pages 15 and 16 for the bri...

Страница 52: ...phragm opening should be performed after centering the diaphragm Centering procedure for the field diaphragm 1 Follow the procedure described in Pages 15 and 16 for the bright field microscopy under the episcopic illumination and focus on the specimen with the 10x objective 2 Lower the field diaphragm open close lever and reduce the opening of the field diaphragm 3 Insert a hexagonal wrench into t...

Страница 53: ...Remove one eyepiece and verify that the aperture diaphragm image is seen in the pupil of the objective in the eyepiece tube 3 Stop down the aperture diaphragm with the A S switch on the operation panel 4 Insert a hexagonal wrench into the aperture diaphragm centering holes on both sides and turn the internal adjustment screws to bring the aperture diaphragm image to the pupil center of the objecti...

Страница 54: ...dification from the registered condition When the aperture diaphragm for the episcopic illumination is modified from the registered condition the EPI switch indicator starts blinking This adjustment amount User offset is stored in the microscope memory therefore the post adjustment state is restored when the registered combination of the microscopy and the objective is selected even after another ...

Страница 55: ...the condenser focus knob to form the field diaphragm image on the specimen surface 4 Turn the condenser centering screws on both sides so that the field diaphragm image is positioned in the center of the field of view 5 Locate the 50x objective into the optical path Turn the fine focus knob to focus on the specimen 6 Turn the condenser focus knob to form the field diaphragm image on the specimen s...

Страница 56: ...ompleting focusing and centering for the condenser lens Aperture diaphragm The aperture diaphragm controls the numerical aperture of the illumination system closely related to the resolution of the optical image contrast and depth of focus Rotating the aperture diaphragm ring lever on the condenser for the diascopic illumination will change the opening of the aperture diaphragm Generally the apert...

Страница 57: ...r a l u n n a h P e d o c h P e h t o t g n i d n o p s e r r o c s e l u d o m e h t f o e n o t c e l e S e h t r e d n u y p o c s o r c i m h P e h t r o f e v i t c e j b o e h t f o e h t r e t n e c n e m i c e p s a e v r e s b o o T n o i t a n i m u l l i c i p o c s a i d 1 e v i t c e j b o e h t f o g n i r e t a l p e s a h p e h t r o f m g a r h p a i d x 4 2 s n e l y r a i l i x ...

Страница 58: ...on can be performed by using the polarizer with the analyzer slider and the DIC slider LV UPO polarizer equipped with the 1 4 lambda plate The LV UPO is used to perform the Senarmont method of DIC microscopy when used with the analyzer and the DIC slider The contrast of the DIC contrast image can be adjusted with the polarizer orientation LV UVPO UV polarizer The polarizer is used to change the ex...

Страница 59: ...tical path and you wish to remove the polarizer from the optical path pull the polarizer slider toward the right to the first click stop position The empty hole will be placed into the optical path Orientation adjustment for the polarizer The orientation of the polarizer can be changed by turning the polarizer rotation ring Perform tgÀêfollowing to make a crossed Nicols position with the polarizer...

Страница 60: ... 5 3 7 0 1 JA P A N Polarizer for the diascopic illumination Dark cross Polarizer C SP polarizer for the diascopic illumination D DP rotatable polarizer for the diascopic illumination Application Polarization microscopy under the diascopic illumination simplified sensitive color DIC microscopy under the diascopic illumination Remark Provided with the swing out mechanism Equipped with the 1 4 lambd...

Страница 61: ...umination to align the orientation indicator with the index 6 Fully open the aperture diaphragm 7 Remove one eyepiece from the eyepiece tube and look inside the open sleeve You can see the pupil of the objective as a bright circle and can see black patterns in the circle 8 Loosen the setscrew on the rotatable polarizer for the diascopic illumination with the hexagonal screwdriver 9 Rotate the rota...

Страница 62: ...C microscopy under the episcopic illumination The DIC microscopy under the diascopic illumination can be performed when the universal condenser the analyzer slider D DP rotatable polarizer for the diascopic illumination equipped with the 1 4 lambda plate the condenser DIC prism and the objective DIC prism are attached to the microscope Additionally the sensitive color DIC microscopy under the dias...

Страница 63: ...ethod of the DIC microscopy cannot be performed Structure of the PA block The PA block has the same shape with the filter cube for the epi fl microscopy The UV filter and the polarizer are mounted on the entrance side and the analyzer is mounted on the exit side The polarizer and the analyzer have been adjusted to the crossed Nicols position PA block installation The PA block is installed in the F...

Страница 64: ... DIC microscopy under the episcopic illumination Placing the lambda plate into the optical path The slot for the lambda plate is provided to the front of the slot for the polarizer of the LV UEPI2A Remove the dummy slider and insert the lambda plate into the place See Page 83 When the slider is pushed into the first click stop position the empty hole is placed into the optical path And when the sl...

Страница 65: ...the lambda plate to the slot for the lambda plate slider of the LV NU5AI see Page 80 Loosen the slider limiter screw with the hexagonal screwdriver provided with the microscope and insert the lambda plate slider into the slot And then tighten the slider limiter screw until it is inserted into the groove To remove the lambda plate slider from the nosepiece loosen the slider limiter screw sufficient...

Страница 66: ...t method is applicable Use the objectives for industrial microscopes marked with LU Selecting the DIC slider Check the DIC prism position indicated on the objective body and select the DIC slider suitable for the objective On the right figure the symbol A is marked at the right of the magnification and the NA value The symbol indicates the DIC prism position for the objective Attaching the DIC sli...

Страница 67: ... knob on the DIC slider to match the letter A with the white circle Interference color You can change the interference color continuously by rotating the prism position knob located at the tip of the DIC slider When the DIC microscopy under the episcopic illumination is performed applicable microscopy method differs depending on the used nosepiece When the motorized universal quintuple nosepiece L...

Страница 68: ...LU nosepiece adapter M32 25 Inserting the condenser DIC prism Attach the condenser DIC prism inside the universal condenser beforehand Rotate the condenser turret to place the condenser DIC prism into the optical path There are three types of condenser DIC prisms N1 Dry N2 Dry and NR Dry Refer to the table on Page 67 to select the DIC prism suitable for the used objective For the procedure of atta...

Страница 69: ...Fluor 20X MI Plan Apo 20X S Fluor 20X Fluor 20X W Plan Fluor 40X Plan Apo 40X S Fluor 40X Plan NCG 40X Plan Fluor 40X Oil Fluor 40X Oil Fluor 40X W Plan Apo 40X Oil Plan Apo 60X Oil A Plan Apo 60X Fluor 60X W Plan Apo VC 60X H CFI APO TIRF 60X Oil Plan Fluor 60X Oil Plan Apo TIRF 60X Oil Plan Apo 60X WI Plan Apo VC 60X WI Plan Fluor 60X A Plan Apo VC 100X H CFI APO TIRF 100X Oil Plan Apo 100X NCG ...

Страница 70: ... product Nikon recommends that the light source to be installed onto this microscope should have been tested by a safety certification organization Selecting the excitation light filter EX filter An excitation light filter transmits lights selectively and blocks other lights The transmitted lights are called excitation lights They are used to excite the fluorophore in the specimen and fluorescent ...

Страница 71: ...cement in filter performance however filters with shorter cut on wavelengths can be used for this purpose and they are used more often than before 2 An LP filter is used for a specimen labeled with multiple wavelengths where fluorescent images for all the wavelengths are desired However the usual combination of a dichroic mirror an excitation light filter and a barrier filter of LP filter type may...

Страница 72: ...ew one and secure it in the reverse order When attaching the barrier filter make sure to place the filter with its arrow mark on the rim facing down to the dichroic mirror side If a filter made by other manufacturer is used check and see the filter orientation with the indication on the rim of the filter before securing it Replacing dichroic mirrors A dichroic mirror is fixed with a flat spring an...

Страница 73: ...ight balancer Remove the vertically oriented cover on the left side of the illuminator And then insert the excitation light balancer into the slot with its mark facing back When the excitation light balancer is inserted to the limit position it enters into the optical path You can adjust the excitation light by sliding the excitation light balancer horizontally Objective To use the excitation ligh...

Страница 74: ...on the excitation light balancer The transmittance for the FITC is designed to keep approximately 100 because the FITC is usually dark fluorescent image Optical path position 1 Between 1 and 2 2 Between 2 and 3 3 TRITC Texas Red 0 Variable 0 to 50 50 Variable 50 to 100 100 DAPI 100 Variable 100 to 50 50 Variable 50 to 0 0 FITC 100 100 100 100 100 III Operation of Each Part ...

Страница 75: ...ion optical instrument Handle it carefully and do not subject it to a strong physical shock In particular objectives may loose accuracy when exposed to even a weak physical shock CAUTION Software setup work When the product is assembled or the configuration of the microscope system is changed perform the software setup works for various settings of the microscope via a PC by using the software LVS...

Страница 76: ...ration Install the product on a sturdy desk or table that is able to bear the weight of the instrument Do not install the product in a hot and humid location Arrange a layout that allows easy removal of the power cord from the inlet of the product in the event of an emergency For details about the operating environment and storage environment see VII Specifications Combination of the illuminator a...

Страница 77: ...urce adapter Light guide fiber 2 12V 50W halogen lamp External light source 2 Power supply 3 LV UEPI2A illuminator or or Condenser Polarizer for the diascopic illumination Rotatable polarizer for the diascopic illumination or Lamp cable Lamp cable 3 Glass slide holder 3x2 stage DIC slider Motorized universal quintuple nosepiece LV NU5AI Motorized universal quintuple nosepiece LV NU5A LV NU5AC Lamb...

Страница 78: ...t positioning plate to the positioning boss part on the top of the condenser holder refer to Page 77 G stage 2 and rectangular stage To attach the P GS2 G stage 2 or the rectangular stage for the product attach the LV SAD stage adapter Align the positioning pin on the bottom of the stage adapter with the groove of the substage insert the truncated cone joint of the stage adapter into the substage ...

Страница 79: ...ew to fix the stage glass or the glass slide holder Take care not to lift up the stage glass by tightening the clamp screw too much 3 Attaching the condenser upper limit positioning plate If the rotatable mechanical stage is directly attached the condenser may hit the stage because the rotatable mechanical stage is thinner than other stages by 1 mm To use the rotatable mechanical stage attach the ...

Страница 80: ...hange the stage glass to the glass slide holder e g a t S r e s n e d n o c e l b a c i l p p A e g a t s 2 x 3 c i t a m o r h c a e d i l s x 0 0 1 2 r e s n e d n o c D W L r e s n e d n o c c i t a m o r h c a r e s n e d n o c e b b A r e s n e d n o c l a s r e v i n u D U C V L d n a r e s n e d n o c n o i t a c i f i n g a m w o l r e s n e d n o c r e s n e d n o c D W L e h t r o f t p ...

Страница 81: ... from the front with aligning it to the groove at the bottom of the microscope arm and slide it toward the back as far as it goes At this step the cable of the nosepiece must be drawn into the microscope through the hole at the bottom of the arm 4 Fix the nosepiece with the nosepiece clamp screw 5 Connect the cable of the nosepiece to the cable inside the arm 6 Put the cover back to its original p...

Страница 82: ...itive color DIC microscopy Attach the lambda plate according to the following procedure 1 Loosen the limiter screw with the hexagonal screwdriver provided with the microscope 2 Insert the lambda plate into the slot 3 Tighten the limiter screw of the nosepiece so that it is inserted into the groove to prevent inadvertent disconnection of the lambda plate To remove the lambda plate from the nosepiec...

Страница 83: ...22 DIC Prism for the Diascopic Illumination of III Opearation of Each Part LV NU5AI motorized nosepiece For the LV NU5AI nosepiece the Senarmont method is used for the DIC microscopy under the epi dia illumination Select the objective DIC prism DIC slider suitable to the illumination method epi dia and the characteristics of the objective Insert the DIC slider into the slot for objectives When the...

Страница 84: ...rovided with the LV UEPI2A 6 Connect the special cable to the connector on the side of the LV UEPI2A and to the UEPI2A connector on the connector panel of the product UEPI2A USB RS232C F STOP J A P A N BF DF FL1 FL2 FL1 FL2 Ultraviolet light shield To be secured with two screws Two hexagonal bolts Connection cable Illuminator clamp screw To disconnect the cable pull the cable while pushing the pro...

Страница 85: ...n To use sliders remove the covers on the slider slots and insert the sliders Note that the slots for the polarizer slider and the dummy slider lambda plate slider share a single cover So when using only the polarizer slider insert a dummy slider in front of the polarizer slider When you don t need any slider attach the covers onto the slider slots 3 Filter sliders and filters 1 Remove each filter...

Страница 86: ...the filter cubes just attached Affix them to the FL1 position and the FL2 position on the microscopy method indicator If you cannot find the corresponding stickers write the excitation methods on blank stickers and affix them 4 Filter cubes The filter cube turret of the LV UEPI2A can accommodate two optical components such as filter cubes for the epi fl microscopy or PA blocks LV PAB J A P A N Cov...

Страница 87: ... buy these lamps please contact your nearest Nikon representative Never touch the glass surface of the lamp with bare hands Doing so will cause fingerprints grease etc to burn onto the lamp surface reducing the illumination If you do get any fingerprints or dirt on the lamp wipe them clean Make sure the lamp house cover is securely fitted to the lamp house after replacing lamps Never turn on the l...

Страница 88: ... house to the connection port on the rear of the illuminator or on the rear of the microscope body and insert the lamp house as far as it goes 3 Using the hexagonal screwdriver supplied with the microscope tighten the clamp screw on the upper side of the connector of the lamp house to secure it 4 Plug the cable coming from the lamp house into the lamp connector on the rear of the microscope and ti...

Страница 89: ...he lamp house cover clamp screw using the hexagonal wrench 2 Remove the lamp house cover 3 Push down the lamp clamp lever and remove the old lamp 4 With the lamp clamp lever held down insert the electrodes of a new lamp into the holes of the socket Insert the lamp as far as it goes and then release the lamp clamp lever to secure the lamp Be careful not to touch the glass surface of the lamp with b...

Страница 90: ...the lamp cable which is provided with the power supply to the lamp cable of the lamp house and to the OUTPUT connector on the power supply 2 Connect the control cable which is provided with the power supply to the EXTERNAL connector on the power supply and to the LCNT connector on the LV100DA 3 Turn the EXTERNAL switch on the back of the power supply to the ON side This disables the brightness con...

Страница 91: ... strong light including ultraviolet light that is harmful to the eyes and skin Never turn on the power for the light source before completion of assembling and connecting parts To assemble and connect parts check that the power supplies for the light source and microscope are turned off and that the power cable is unplugged from the wall outlet When you use the diascopic illumination with this pro...

Страница 92: ...r t 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numérique de la classe A est confirme à la norme NMB 003 du Canada JAPAN C HGFIE 870...

Страница 93: ...st be turned on with the LV UEPI2A attached So this step must be performed after the assembly of the microscope 1 When the light source the lamp house or the optical fiber light source is attached to the microscope turn off the light source And if possible turn off the power for the light source 2 Plug the power cord and turn on the product 3 Remove the cover on the left side of the LV UEPI2A 4 Ch...

Страница 94: ...nob on the right To tighten the clamp screw insert the hexagonal screwdriver through the cooling slit into the adapter and tighten the clamp screw inside 3 Attach the lamp house to the lamp house mounting part of the double light source adapter as far as it goes and tighten the clamp screw securely with the hexagonal screwdriver For the connection of the lamp cable see Page 85 Attaching the Lamp H...

Страница 95: ...3 To remove an objective perform the following remove the specimen from the stage lower the stage completely and hold the objective with both hands so that it does not fall during the removal 9 Attaching Eyepieces Attach eyepieces of the same magnification and the same field number There are positioning protrusions on the binocular part sleeve of the eyepiece tube Align the notches of the eyepiece...

Страница 96: ...e the polarizer onto the field lens on the base of the microscope The orientation indicator or the index must face the front of the microscope 3 Tighten the setscrew to fix the polarizer The orientation of the polarizer must be adjusted for each observation F S 7 5 3 7 0 1 JA P A N Polarizer for the diascopic illumination 7 5 3 7 0 1 JAPAN Swing out center Orientation indicator Setscrew C SP polar...

Страница 97: ...the stage when observing a thick specimen It is attached between the arm and the stage of the product When a column riser is attached the objective height rises 35 mm Attaching a column riser 1 Remove the illuminator the eyepiece tube and the nosepiece when they are attached on the arm Be careful not to drop them 2 Remove four hexagonal socket head bolts which fix the arm of the microscope to the ...

Страница 98: ...ated in the connector panel on the side For details about the operation and the setup works of the LVSetup refer to the LV Series Support Tools software manual PC USB cable UEPI2A USB RS232C LCNT ND8 NCB F S Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT LV TT2 Connecting with a PC when using the NIS Elements Moto...

Страница 99: ... is used for the communication between the DS L2 and the microscope Connect the USB cable between the USB connectors of the DS L2 and the microscope For the operation procedure of the DS L2 see the instruction manual for the DS L2 UEPI2A USB RS232C LCNT ND8 NCB F S Achr N A 0 9 JAPAN 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 3 x 2 S TA G E JA PA N F STOP JA P A N BF DF FL1 FL2 FL1 FL2 100 20 0 100 IN OUT LV...

Страница 100: ... parts of the microscope have anti static finishes which are very useful when observing electrostatically sensitive specimens The anti static parts include the LV100DA U the LV UEPI2A epi illuminator the LV TT2 eyepiece tube the L W 10x eyepieces the 3x2 stage the ESD plate the motorized universal quintuple nosepiece and the objectives The ground connection can be taken through the 3 conductor pow...

Страница 101: ...s focused on the specimen surface p 53 Center the condenser p 53 Install the condenser correctly p 78 Rotate it to the click stop position p 55 Move the upper part to a click stop position p 59 Cause The lamp is not attached correctly The optical path selector lever on the eyepiece tube is in an intermediate position The optical path selector lever on the eyepiece tube is not placed to the positio...

Страница 102: ...e objective into the optical path p 43 Attach the specimen holder correctly p 77 Install the nosepiece correctly p 79 Push the OBJ switch several times to move the objective into the optical path p 43 Attach the stage correctly p 76 Install the microscope on a flat and level surface Center the condenser p 53 Cause The aperture diaphragm is stopped down too far Dirt or dust exists on the lens eyepi...

Страница 103: ...m image is focused on the specimen surface p 53 Adjust the diopters p 49 Mount the eyepieces correctly by aligning the positioning grooves p 93 Secure the specimen holder correctly p 77 Adjust the interpupillary distance p 49 Adjust the diopters p 49 Adjust the distance p 49 Adjust the diopters p 49 Adjust the brightness with the brightness control switch or ND filters p 42 and 44 Use eyepieces ha...

Страница 104: ... the DIC prism suitable for the objective into the optical path p 66 and 67 Focus the condenser p 53 Plase it into the optical path p 62 and 63 Center the Ph annular diaphragm until it coincides with the phase plate ring Refer to the instruction manual for the LV CUD Place the Ph annular diaphragm specified by the Ph code of the objective p 55 Focus the condenser p 53 Cause The coarse torque adjus...

Страница 105: ...oscope p 36 Turn on the power supply p 35 Connect the cable correctly p 88 Change the EXTERNAL switch position to ON p 88 Perform the setup works for the microscope p 36 Turn on the light source p 35 Connect it properly p 89 Connect it properly p 90 Perform the setup works for the microscope p 36 Cause The lamp is about to blow The power cord or the cable of the lamp house is not connected securel...

Страница 106: ...ect the control cable correctly and turn the EXTERNAL switch to the ON position p 88 Perform the setup works for the microscope p 36 Connect the RS 232C cable p 90 Disable the interlock controls Refer to LV Series Support Tools software manual Perform the setup works for the microscope p 36 Enable the interlock controls Refer to LV Series Support Tools software manual Perform the setup works for t...

Страница 107: ...tive Interlock controls are enabled The information of the microscope configuration is not registered correctly Interlock controls are disabled The information of the microscope configuration is not registered correctly Motorized nosepiece Problem The aperture diaphragm for the episcopic illumination does not change even though the aperture diaphragm open close switch is pressed The filter cube tu...

Страница 108: ...s eyepieces and glass plate according to the procedure described in 1 Cleaning Lenses and Filters without removing them from the microscope Nikon recommends cleaning them frequently Clean the objectives filters and the like to maintain the optical performance When cleaning the objectives remove them from the microscope Clean them whenever they are contaminated Microscopes and stages are contaminat...

Страница 109: ...eaning the Painted Parts Plastic Parts and Printed Parts Do not use organic solvents such as alcohol ether or paint thinner on painted components plastic components or printed components Doing so could result in discoloration or in peeling of the printed characters For persistent dirt dampen a piece of gauze with neutral detergent and wipe gently 3 Storage Store this product in a dry place where m...

Страница 110: ...ne focus knob mechanism left side with coarse fine focus right side with coarse focus calibration marking for fine focus 1 µm marking Stroke 40 mm with coarse focus stopper mechanism Coarse focus knob 14 mm revolution Fine focus knob 0 1 mm revolution 10x field number 22 25 UEPI2A connector for LV UEPI2A epi illuminator USB connector for PC to perform setup work for the DS L2 USB 1 1 compatible RS...

Страница 111: ...asonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in whi...

Страница 112: ...ter bright field microscopy Projected magnification 3 0x on the eyepiece image plane Full open diameter for dark field microscopy 9 8 mm Centering range 2 4 mm in diameter Adjustment motorized Variable range 1 2 to 8 0 mm in diameter bright field microscopy Projected magnification 1 55x on the objective pupil plane Full open diameter for dark field microscopy 8 5 mm Centering range 2 4 mm in diame...

Страница 113: ...e attachment position of the objective barrel and the attachment reference position of the nosepiece 53 mm Sliding dovetail reference dimension 50 mm Dovetail five locations Each objective address has a slot for it Slot size 16 5 mm x 24 mm Built in swing out mechanism for the bright field dark field optical path separation ring interlocked with the lambda plate slider insertion Connector of 12 pi...

Страница 114: ...le angle 15 Dimension of objective thread M32 x 0 75 mm Distance between the attachment position of the objective barrel and the attachment reference position of the nosepiece 48 mm Sliding dovetail reference dimension 50 mm 16 5 mm x 24 mm One fixed objective and four centerable objectives Centering screw two M3 setscrews two screws for each objective Centering range 0 3 mm in diameter Connector ...

Страница 115: ...ng to EU EN standards 3 conductor grounding type 3 conductor grounding Type H05VV F 3m long maximum rated at 250 VAC minimum For Japan Power cord set approved according to the Electrical Appliance and Material Safety Law with the PSE mark 3 conductor grounding Type VCTF 3x0 75 mm2 3 m long maximum rated at 125 VAC minimum 12 VDC 100 W 8 4 A 250 V T4A Temperature 0 C to 40 C Humidity 85 relative hu...

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