Chapter 6
Troubleshooting
16
6.5
6.5
View of Images
Symptom
Possible cause
Action
The image blurs.
The optical device is out of focus.
Bring the optical device into focus by operating the
focus drive or focusing ring.
The aperture diaphragm is stopped
down too far when using the
microscope.
Adjust the aperture diaphragm to 70 to 80 percent of
the numerical aperture of the objective, or 70 to 80
percent of the pupil of the objective.
The magnification setting for the optical
device is too high.
Lower the magnification setting for the optical device.
Note: As a guide, use 500 to 1000 times the
numerical aperture of the objective for the
magnifying power.
The electronic zoom is being used for
observation.
Turn off the electronic zooming function.
Note: Use of the electronic zoom may cause an
image to blur depending on the magnification.
The exposure time is too long.
Set a higher value for the camera gain and a lower
value for the exposure time.
Note: If setting the exposure time long for a subject in
motion, the subject is blurred.
The product is used in a place subject
to frequent vibration.
Use the system in a stable, vibration-free place.
The image is too dark or too
bright.
Illumination for the subject is improper.
Adjust brightness of the illumination properly.
The exposure is not adequate.
In AE mode, perform exposure compensation. Or, set
the photometry area and photometry mode properly.
Note: Refer to the instruction manual of the
NIS-Elements.
Monitor is not properly adjusted.
Adjust the display quality of the monitor properly.
Note: Refer to the instruction manual of the monitor.
The contrast is low.
The illumination is too bright or too
dark.
Adjust brightness of the illumination properly.
Adjust the exposure.
Light around the subject affects
contrast.
Darken the interior, or put the cap on the binocular
tube for a microscope.
Field diaphragm and aperture
diaphragm of the optical device
(microscope) are set improperly.
Adjust the field diaphragm to near the size
circumscribing the field. Adjust the aperture
diaphragm to 70 to 80 percent of the numerical
aperture of the objective.
Filter selection for the optical device
(microscope) is improper.
For a phase contrast microscope or interference
microscope (dual beam or multiple), insert the
specified filter.
Note: For negative-positive images, generally,
inserting a green interference filter (GIF)
improves contrast.
Note: Insert a complementary color filter of the color
of the specimen for stronger contrast.
The contrast of the subject itself is low.
Consider illumination and/or microscopy method to
compensate for the low contrast.
The image adjustment is improper.
Set the saturation, hue, contrast, black level, etc. to
an appropriate level.
Note: Refer to the instruction manual of the
NIS-Elements.
Monitor is not properly adjusted.
Adjust the display quality of the monitor properly.
Note: Refer to the instruction manual of the monitor.
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