Operator’s Manual for the Bullet Blender
®
4
you wish to save until completion. At
this time a list of options will appear.
Select the "save protocol" option. The
instrument will then prompt you to
name protocol you wish to save. Use
the arrow keys and scroll knob to
navigate through the character list.
When you are finished naming the
protocol select the check mark to
continue.
The
instrument
will
automatically assign the nearest
empty slot in your saved protocols
list. Alternatively, you can enter the
saved protocols list and choose an
empty slot for edit. You will then just
need to change the name and select
the correct settings.
PROTOCOLS AND SAMPLE
SETTINGS
Custom
homogenization
protocols can be uploaded to your
Bullet Blender 50-DX using a USB
flash drive. Contact customer support
to obtain the necessary protocol files.
Once the files have been obtained
load them onto your USB flash drive.
Plug the flash drive into the USB
outlet and power cycle (turn off and
on again) your instrument. The Bullet
Blender will recognize the protocol
file on the flash drive and prompt you
to save. If you choose to save the
protocol file, you must then choose
an empty slot for the in the saved
protocols
list.
You
have
now
successfully
saved
your
new
protocol.
The following sample, bead to
buffer ratio should be used as a
general
guideline
for
achieving
optimal homogenization quality - 1X
volume/mass of tissue: 1X volume of
beads: 2X volumes of buffer. For
more specific information regarding
specific protocol information, please
contact
customer
support
at
For 50 mL tubes, do not operate
with more than 3.5g of sample, or a
total of 20mL of buffer, tissue, and
beads (per tube). Do not operate the
Bullet Blender 50-DX using the same
tubes for more than 30 minutes.
Cutting the tissue into smaller
pieces will generally yield better
results. Tissue with a high aspect
ratio
(long,
thin
strips)
will
homogenize better than tissue that is
round or cubic.
Note:
Use
TPP
®
brand
polypropylene, skirted (self-standing)
50 mL tubes. Some discoloration of
the
sleeves
and
tubes
after
homogenization is normal and a
byproduct of the workings of the
Bullet Blender 50-DX.
CLEANING
If you wish to clean your Bullet
Blender, clean the outside of the unit
only with mild detergent, water
alcohol and a soft cloth. Under
normal conditions, the Bullet Blender
50-DX should never need to be
disassembled for cleaning. If needed
unplug the instrument, remove the
