5
OBJECTIVE
DIAPHRAGM OPENING
4x
From fully closed to 1/8 open
10x
1/8 to 1/4 open
40x
1/4 to 1/2 open
100x
1/2 to 3/4 open
F. Usage of filters (one 32mm O.D. transparent blue filter and one frosted neutral filter has been provided
with your microscope)
a) Placing a filter into the optical path will absorb some of the light from the illuminator base, limiting the
light output. Filters should only be used when they help enhance the image or to help reduce
brightness of field.
b) To insert filter, grasp filter holder knob and swing out filter holder, insert filter into slot provided and
swing filter into optical path.
c) When handling the filter do not touch lens surfaces as fingerprints and smudges will result in poor
images.
G. Changing magnification.
a) Rotate revolving nosepiece to position 10x objective into optical path.
b) This microscope has been parfocalized, which allows changes from one objective to another while
requiring only a slight adjustment of the fine focus controls.
c) When changing to the 40x and 100x objective lens, care must be exercised when positioning these
lenses into the optical path, in order to prevent damaging the front lens element and specimen slide.
d) In order to obtain maximum resolution of the 100x oil immersion lens, it is necessary to apply
immersion oil between the coverglass of slide and front lens of the objective.
(1) Use of a very small amount of immersion oil is required.
(2) All air bubbles must be removed from between lens and slide by gently rotating nosepiece back
and forth.
(3) When finished viewing, all parts that come in contact with oil must be cleaned. Failure to do so
could permanently damage the 100x oil objective lens. Use of Windex to clean immersion off
lens surfaces is recommended.
Microscope Specification Chart
Objective
N.A.
Color Code
Ring
Field of
View
Working
Distance
Magnification with
WF10X eyepiece
Din 4X
0.10
Red
4.5mm
26.4mm
40X
Din 10X
0.25
Yellow
1.8mm
5.5mm
100X
Din 40X
0.65
Blue
0.45mm
0.48mm
400X
Din 100X
1.25
White
0.18mm
0.06mm
1000X
