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reduces any bicarbonate alkalinity in the sample and prepares
it for titration.
After 120 seconds, “
add
” alternating with “
h1
1
” will display. (The “
1
” indicates the first addition of sodium
hydroxide.) Then “
agit
” alternating with “
hOLd
” will display.
13.
Change the pipette tip.
14.
Using the pipette, add 100µL of Reagent H1 to the cell,
close the cell extender cap securely to prevent any solution
from spilling out, then agitate and hold. Repeat as prompted.
“
P
r
ES caL
” will display.
15.
Press
caL
.
“
add
” alternating with “
h1 2
” will display. (The
“
2
” indicates the second addition of sodium hydroxide.) Then
“
agit
” alternating with “
hOLd
” will display.
16.
Tap the cell extender cap to dispel any solution clinging to the
cap back into the cell. Carefully open the cell extender cap so
as not to spill any solution.
17.
Using the pipette, add a second 100 µL of Reagent H1 to the
cell, close the cell extender cap securely to prevent any solution
from spilling out, then agitate and hold. Repeat as prompted.
“
P
r
ES caL
” will display.
18.
Press
caL
to advance to first titration prompt. “
add
” alternating
with either “
EdTa Lc
” (Reagent H2) or “
EdTa hc
” (Reagent
H3) will display; then “
agit t1
” alternating with “
hOLd
” will
display. The number after “
t
” indicates which titration point you
are on. The number “
1
” here indicates this is the first titration
point. Subsequent titration points are sequenced numerically: 2,
3, 4, etc.
19.
Tap the cell extender cap to dispel any solution clinging to the
cap back into the cell. Carefully open the cell extender cap so
as not to spill any solution.
20.
Change the pipette tip.
21.
Using the pipette, add 100µL of Reagent H2 if
“
EdTa Lc
”
is
displayed, or H3 if
“
EdTa hc
”
is displayed.
22.
Close the cell extender cap securely to prevent any solution
from spilling out. Then agitate and hold. Repeat as prompted.
“
P
r
ES caL
” will display.
23.
Press
caL
to advance to the next titration point. Continue
adding 100µL of Reagent H2 or H3 using the pipette,
agitating
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