ML41biological microscope user manual
14
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3.2 Put slices
(1)Gently push back the wrench
①
on the slicer.
(2)Put the cover glass of the sample
②
into the slide holder, and release
the spanner
①
to clamp the slice firmly.
(3)Turn the X and Y axis movement knobs
③
of the stage to move the
slice to the center of the field of view and align it with the center of
the objectives. (Picture 10)
3.3 Focusing
(1)Turn the 4X objective into the optical path.
(2)Loosen the coarse limit hand wheel
①
, observe the right eyepiece,
turn the coarse hand wheel
②
until the outline of the specimen appears
in the field of view, and then lock the coarse limit hand wheel in the
direction shown in the picture.
☆
The coarse adjustment limit hand wheel can prevent the slice
and the objective lens from colliding during focusing.
☆
Coarse adjustment limit hand wheel has no effect on micro
hand wheel.
(3)Turn the micro-hand wheel
③
to clearly observe the details of the
specimen. (Picture 11)
☆
When using the 4X and 10X objectives, the aperture diaphragm
and field diaphragm are opened to the maximum, and the condenser is
moved out of the light path. Operation of the condenser is shown in
"3.8 Condenser Centering".
3.4 Adjustment of the tightness of the focusing
If the hand feel is heavy during rough focusing, or the specimen
leaves the focal plane due to the automatic lowering of the stage after
focusing, it can be solved by turning the elastic adjustment ring
①
,
and the focus of the focusing mechanism can be changed by rotating
according to the picture. (Picture 12)
