─
5
─
③
Turn on the switch on the back of the instrument, the instrument runs self-test, and can be
tested after completion.
4. Precautions for instrument operation
This fluorometer can use cuvettes, 0.2mL fluorescent PCR tubes, and 0.5mL fluorescent
PCR tubes for sample detection. Corresponding tube holder should be configured when using
fluorescent PCR tube for detection.
Sample testing steps:
1. As shown in the figure below (Figure 3), insert the PCR tube holder (1) first
—
it has
been inserted in the general instrument;
2. Then insert the PCR tube with the fluorescent sample into the PCR tube holder;
3.Cover the sample cover and click "detecting" in the interface to perform sample
detection
Figure 2 Sample placement
In order to ensure the detection effect of the instrument, when using a 0.2mL fluorescent
PCR tube, the sample volume is 0.1mL; when using a 0.5mL fluorescent PCR tube, the
sample volume is 0.2mL; if the sample volume is not enough, the corresponding solvent can
be used to dilute the sample before testing. .
But be careful when diluting, if the concentration is too low, it will affect the stability of
sample detection. And the amount of solution in the tube when the sample is detected should
be the same as the volume established by the standard curve.
Fluorescent PCR tube
PCR tube socket