background image

Operation

 

Manual

Chapter 1

Introduction

1

Chapter

 

1

 

Introduction

 PCR-IF-8C 

Isothermal

 

Fluorescence

 

PCR

 

is

 

contain

 

temperature

 

control

 

block,

  

fordoptical

 

path

 

detection

 

system,

 

heating

 

lid

 

and

 

analysis

 

software.

 

It

 

is

 

use

 

d

 

cannisothermal

 

amplification

 

and

 

fluorescence

 

real-time

 

detection

 

of

 

samples,

 

a

 

s

 

widelyimake

 

quantitative

 

and

 

qualitative

 

analysis

 

of

 

the

 

amplification

 

result.

 

It

 

 

i,gused

 

in

 

rapid

 

detection

 

of

 

pathogenic

 

microorganisms(viruses,

 

bacteria,

 

fun

mycoplasma,

 

parasites

 

etc.),

 

food

 

hygiene

 

inspection,

 

environmental

 

monitoring,

 

sex

 

identification

 

of

 

animal

 

embryos

 

and

 

detection

 

of

 

genetically

 

modified

 

food,

 

etc.

Working

 

principle:

Temperature

 

control

 

block

 

of

 

the

 

instrument

 

heats

 

the

 

sample

 

seat

 

to

 

heat

 

the

 

tube

 

in

 

the

 

seat

 

to

 

the

 

needed

 

temperature.

 

The

 

heating

 

lid

 

function

 

can

 

effectively

 

inhibit

 

the

 

evaporation

 

of

 

reagent

 

and

 

keep

 

the

 

reaction

 

system

 

constant

 

without

 

adding

 

paraffin

 

oil.

 

The

 

optical

 

path

 

detection

 

system

 

can

 

collect

 

the

 

fluorescence

 

intensity

 

of

 

the

 

DNA

 

containing

 

dye

 

or

 

probe

 

in

 

the

 

test

 

tube

 

at

 

a

 

set

 

time

 

interval.

 

The

 

analysis

 

software

 

can

 

process

 

and

 

transform

 

the

 

collected

 

signals,

 

and

 

finally

 

output

 

the

 

experimental

 

results.

Principle

 

of

 

optical

 

path

 

detection

 

system:

1: Principle diagram of optical path detection system;

21: Plus lens;

22: Excitation filter; 23: Excitation light source

31: Collecting lens; 32: Emission filter; 33: Detector

Содержание PCR-IF-8C

Страница 1: ...Operation Manual PCR IF 8C Isothermal Fluorescence PCR PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC 2 22...

Страница 2: ...I...

Страница 3: ...software operation of the instrument simple fault analysis transportation and storage requirements etc This instrument is non IVD instrument It is limited to scientific research and teaching purposes...

Страница 4: ...instrument conform to class of GB 4793 1 standard Suggest use this instrument indoors or outdoors place where avoid light Disassemble the instruments without permission is forbidden This will make yo...

Страница 5: ...atform where away from water and dust during the outdoors using The instrument should be kept in a cool clean place and avoid directly sunlight strong light to make sure Fluorescence detection can be...

Страница 6: ...tion hole and disinfect the instrument with ultraviolet light before transport please Retain the original package for transport using to avoid damage during transportation Warning label Warning Hot su...

Страница 7: ...2 Features 2 Chapter 3 Accessories List 4 Chapter 4 System structure and accessories 5 Chapter 5 Operation Guide 8 Chapter 6 Fault analysis and solutions 17 Chapter 7 Maintenance storage and transport...

Страница 8: ...of genetically modified food etc Working principle Temperature control block of the instrument heats the sample seat to heat the tube in the seat to the needed temperature The heating lid function ca...

Страница 9: ...em 10 150 L Recommended 20 30 L Power supply Power Max AC100 240V 50 60Hz 100VA Dimensions W D H Max 145 305 100mm Weight Max 2 5kg Temperature control Heating lid function Yes temperature is adjustab...

Страница 10: ...Features 3 Fusion function Analysis of melting curve and melting peak Result display mode 1 positive negative 2 peak time 3 Tm value Optional software PC software Software upgrade Picture updated U d...

Страница 11: ...form is selected according to the order 3 Power adapter pcs 1 4 Operation manual pcs 1 1 each 5 USB flash disk contain software pcs 1 Software included according to order requirements 6 USB cable USB...

Страница 12: ...y introduces the structure of the instrument kit installation as well as the preparatory work before starting up Please have a comprehensive understanding about the instrument before the first time us...

Страница 13: ...ation Manual Chapter 4 System structure and accessories 6 4 3 Power adapter 4 4 USB cable USB flash disk 4 5 Thermal printer Disclaimer the above picture is for indication only subject to actual deliv...

Страница 14: ...ating lid gently it will be closed well when listening click sounds Fig 4 4 Schematic diagram of sample installation Notice The instrument is forbidden to mix centrifugal tubes in one operation to avo...

Страница 15: ...ault is Detection Interface Isothermal amplification 5 2 Fast start experiment Name Button Description Test Store and summarize new settings or running program files read section 5 4 Data Store and su...

Страница 16: ...on the pop up dialog the name of the original program file will not change As shown in figure Click OK to enter the running interface 1 Temperature setting Click Test Settings Experiment Setting inte...

Страница 17: ...he appropriate experimental channel according to the reagent dye wavelength Channel 1 FAM SYBR Green 4 Setting of peak head time parameters The peak time Tt of the amplification curve is related to th...

Страница 18: ...e changed The selected channel conditions and peak time settings negative and positive settings can be viewed in real time The real time sample holder temperature progress bar remaining time and total...

Страница 19: ...ent curve of the hole Click Null and all curves will be hidden Note The hole color can be set in color read section 5 6 4 Anneal curve It is used to display anneal curves in real time which is divided...

Страница 20: ...interface the parameters of peak time and negative and positive can be edited and the corresponding results will be updated Data save as Data files saved format File name and saved time Data manage C...

Страница 21: ...etting the interface included Experiment program new manage instrument setting and information system setting function etc As shown in figure Experiment setting function please read section 5 3 Sample...

Страница 22: ...he original color of the instrument Return the previous interface It will remind users if not save Language Setting Choose Chinese or English in Language setting interface click to switch it after sel...

Страница 23: ...m setting include Battery Lid Temperature Time Screen Update Maintain Maintain It is a professional option for maintenance personnel or instrument developers to maintain and calibrate instruments Plea...

Страница 24: ...ct temperature value Sensor failure Contact with distributor 4 Sample holder heating lid temperature alarm or not work Thermistor failure Contact with distributor 5 The cooling fan doesn t work sounds...

Страница 25: ...Capture chip read failed Module temperature Module temperature sensor open Module temperature sensor error Module temperature sensor short Module temperature sensor error Heating lid temperature Lid t...

Страница 26: ...s any foreign matter to clean users should wipe it slowly by the balloons Turn off the heating lid to prevent the reaction hole from the dust when not use Wipe the sample hole with clean soft cloth wi...

Страница 27: ...ects our company will be responsible for replacement Twelve months from the date of delivery provide warranty for faults due to material and manufacturing defects During the warranty period our compan...

Страница 28: ...tions and Tags Abbreviations The following Abbreviations are for reference and will appear in this operation manual A Ampere AC Alternating current DC Direct current V Volt Hz Hertz W Watt USB Univers...

Отзывы: